Seyed Hadi EBRAHIMI, Reza VALIZADEH, Zohreh ZARNEGAR, Abbas Ali NASERIAN

Chemical composition and in vitro rumen fermentation of ensiled sugar industry coproducts

The current study aims to compare sugar beet leaves (SBLs) ensiled with sugar beet pulp (SBP), sugarcane molasses (SM),or rock candy juice (RCJ) using an in vitro gas production technique. Treatments in the first group included 10% SBP (control 1) andcontrol 1 plus 5% SM or RCJ. In the second group, there were 3 treatments, including 20% SBP (control 2) and control 2 plus 10% SMor RCJ. The silages with no added soluble carbohydrate (SC) sources had significantly higher (P < 0.001) pH, buffer value index, andammonia nitrogen values than the other treatments. However, there was no significant difference between RCJ and SM in their effecton these variables. Adding both levels of SC sources caused an increase in dry matter digestibility compared to those that only includedSBL and SBP (P < 0.001). Maximal and rate of gas production from slowly fermentable fraction (a2) were significantly greater when thesubstrate contained 85%, 10%, and 5% SBL, SBP, and SC sources, respectively. Using dried SBP and SC sources for the preservation offresh SBL is recommended; however, with higher levels of SC, the silage may be at risk of spoilage, and it must be less exposed to air.

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1. Ohuchi Y, Ying C, Lateef SA, Ihara I, Iwasaki M, Inoue R, Umetsu K. Anaerobic co-digestion of sugar beet tops silage and dairy cow manure under thermophilic condition. J Mater Cycles Waste 2015; 17: 540-546.
2. Baker CJL, Eden A. Studies on the oxalate contents of the leaves of certain varieties of Beta vulgaris. J Agric Sci 1954; 44: 394-399.
3. Cho JG, Gebhart CJ, Furrow E, Lulich JP. Assessment of in vitro oxalate degradation by Lactobacillus species cultured from veterinary probiotics. Am J Vet Res 2015; 76: 801-806.
4. Malavanh C, Preston TR, Ogle B. Ensiling leaves of Taro (Colocasia esculenta (L.) Shott) with sugar cane molasses. Livest Res Rural Dev 2008; 20.
5. Gebrehanna MM, Gordon RJ, Madani A, VanderZaag AC, Wood JD. Silage effluent management: a review. J Environ Manage 2014; 143: 113-122.
6. Münnich M, Khiaosaard R, Klevenhusen F, Hilpold A, Khol- Parisini A, Zebeli Q. A meta-analysis of feeding sugar beet pulp in dairy cows: effects on feed intake, ruminal fermentation, performance, and net food production. Anim Feed Sci Technol 2017; 224: 78-89.
7. Moore CA, Kennedy SJ. The effect of sugar beet pulp-based silage additives on effluent production, fermentation, in-silo losses, silage intake and animal performance. Grass Forage Sci 1994; 49: 54-64.
8. Balakhial A, Naserian A, Heravi Moussavi A, Eftekhar Shahrodi F, Valizadeh R. Changes in chemical composition and in vitro DM digestibility of urea and molasses treated whole crop canola silage. J Anim Vet Adv 2008; 7: 1042-1044.
9. Romero-Huelva M, Ramírez-Fenosa MA, Planelles-González R, García-Casado P, Molina-Alcaide E. Can by-products replace conventional ingredients in concentrate of dairy goat diet? J Dairy Sci 2017; 100: 4500-4512.
10. Muck RE, Weinberg ZG, Contreras-Govea FE. Silage extracts used to study the mode of action of silage inoculants in ruminants. Agric Food Sci 2013; 22: 108-114.
11. Wang J, Chen L, Yuan X, Guo G, Li J, Bai Y, Shao T. Effects of molasses on the fermentation characteristics of mixed silage prepared with rice straw, local vegetable by-products and alfalfa in Southeast China. J Integr Agric 2017; 16: 664-670.
12. Kromann RP, Meyer JH, Stielau WJ. Steam distillation of volatile fatty acids in rumen ingesta. J Dairy Sci 1967; 50: 73- 76.
13. Menke K., Steingass H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Develop 1988; 28: 7-55.
14. Association of Official Analytical Chemists International. Official Methods of Analysis of AOAC International. 17th ed. Arlington, VA, USA: AOAC; 2006.
15. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991; 74: 3583-3597.
16. Ebrahimi SH, Mohini M, Singhal KK, Heidarian Miri V, Tyagi AK. Evaluation of complementary effects of 9,10-anthraquinone and fumaric acid on methanogenesis and ruminal fermentation in vitro. Arch Anim Nutr 2011; 65: 267- 277.
17. Gurbuz Y, Kaplan M. Chemical composition, organic matter digestibility, in vitro gas production characteristics and ensilling of sugar beet leaves as alternative feed resource. J Anim Vet Adv 2008; 7: 1568-1574.
18. FAO. Sugar Beet Tops. Rome, Italy: FAO; 2018.
19. Thibault JF, Guillon F, Rombouts FM. Gelation of sugar beet pectin by oxidative coupling. In: Walter RH, editor. The Chemistry and Technology of Pectin. San Diego, CA, USA: Academic Press; 1991. p. 120.
20. Łozicki A, Koziorzębska A, Halik G, Dymnicka M, Arkuszewska E, Niemiec T, Bogdan J. Effect of ensiling pumpkin (Cucurbita maxima D.) with dried sugar beet pulp on the content of bioactive compounds in silage and its antioxidant potential. Anim Feed Sci Technol 2015; 206: 108-113.
21. McCullough ME. New Trends in Ensiling Forages. Rome, Italy: FAO Publications Division; 1978.
22. Greenhill WL. The buffering capacity of pasture plants with special reference to ensilage. Aust J Agric Res 1964; 15: 511- 519.
23. McDonald P, Edwards RA, Greenhalgh JFD, Morgan CA, Sinclair LA, Wilkinson RG. Silage. In: McDonald P, Edwards RA, Greenhalgh JFD, Morgan CA, Sinclair LA, Wilkinson RG, editors. Animal Nutrition. 7th ed. London, UK: Prentice Hall; 2011. pp. 499-520.
24. Mahala AG, Khalifa IM. The effect of molasses levels on quality of sorghum (Sorghum bicolor) silage. Res J Anim Vet Sci 2007; 2: 43-46.
25. Yuan X, Dong Z, Desta ST, Wen A, Zhu X, Rong T, Shao X, Yuan Z, Dong ST, Desta A. Adding distiller’s grains and molasses on fermentation quality of rice straw silages. Ciencia Rural 2016; 46: 2235-2240.
26. Rooke JA, Lee NH, Armstrong DG. The effects of intraruminal infusions of urea, casein, glucose syrup and a mixture of casein and glucose syrup on nitrogen digestion in the rumen of cattle receiving grass-silage diets. Br J Nutr 1987; 57: 89-98.
27. Khalili H, Huhtanen P. Sucrose supplements in cattle given grass silage-based diet. 2. Digestion of cell wall carbohydrates. Anim Feed Sci Technol 1991; 33: 263-273.
28. Harland JI. By-products. In: Cooke DA, Scott RK, editors. The Sugar Beet Crop. 1st ed. London, UK: Chapman & Hall; 1993. pp. 619-620.
29. Gomes MAB, Moraes GV de, Jobim CC, Carlesso dos Santos T, Rogério Oliveira M, Rossi RM. Aerobic stability, chemical composition and ruminal degradability of sugarcane silage with glycerin from biodiesel. Semina Ciências Agrárias 2015; 36: 1531-1544.
30. Wilkinson JM, Davies DR. The aerobic stability of silage: key findings and recent developments. Grass Forage Sci 2013; 68: 1-19.