Fateh SINGH, Ganesh Gangaram SONAWANE, Jyoti KUMAR, Shivendra Kumar DIXIT, Rajendra Kumar MEENA, Bhupendra Nath TRIPATHI

Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India

Öz The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep and goats and to determine antimicrobial resistance (AMR) patterns of the isolates and evaluate them for the presence of extended-spectrum ß-lactamase (ESBL) using the phenotypic and molecular methods. Pneumonic lung tissues and heart blood samples were collected by performing necropsy on sheep (n = 96) and goats (n = 08). The samples were processed and used for bacterial isolation. E. coli isolates (n = 58) including 53 from 34 sheep and 5 from 3 goats were recovered and identified by the cultural and biochemical characteristics and 16S rRNA sequencing. The isolates were tested to ascertain AMR pattern, plasmid features, and ESBL production. The highest rate of resistance (65.5%) was recorded against amoxicillin, enrofloxacin, norfloxacin, and ofloxacin. Eighteen (31%) out of 58 isolates showed the ESBL phenotype by combination disk assay. There was a variability in the number (1-5) and size (2 to >20 kb) of plasmids among isolates. Polymerase chain reaction (PCR) amplification revealed the presence of blaH$_{TEM}$ (70.6%) and blaH$_{SHM}$(1.7%) genes. PCR specificity was confirmed by nucleotide sequencing. The present study indicates that the extraintestinal and multidrug-resistant (MDR) E. coli strains were associated with pneumonia and/or septicemia in sheep and goats. Therefore, thorough surveillance and monitoring of MDR bacteria are urgently needed to implement the infection control strategies and to restore the efficacy of available antibiotics.

Anahtar Kelimeler:

Escherichia coli, extended-spectrum ß-lactamase, multidrug resistance, pneumonia, sheep, septicemia

Tam Metin

Kaynak Göster

Bibtex	@ { tbtkveterinary663783, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2019}, volume = {43}, pages = {754 - 760}, doi = {}, title = {Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India}, key = {cite}, author = {Sıngh, Fateh and Sonawane, Ganesh Gangaram and Kumar, Jyoti and Dıxıt, Shivendra Kumar and Meena, Rajendra Kumar and Trıpathı, Bhupendra Nath} }
APA	Sıngh, F , Sonawane, G , Kumar, J , Dıxıt, S , Meena, R , Trıpathı, B . (2019). Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India . Turkish Journal of Veterinary and Animal Sciences , 43 (6) , 754-760 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/50881/663783
MLA	Sıngh, F , Sonawane, G , Kumar, J , Dıxıt, S , Meena, R , Trıpathı, B . "Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India" . Turkish Journal of Veterinary and Animal Sciences 43 (2019 ): 754-760 <https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/50881/663783>
Chicago	Sıngh, F , Sonawane, G , Kumar, J , Dıxıt, S , Meena, R , Trıpathı, B . "Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India". Turkish Journal of Veterinary and Animal Sciences 43 (2019 ): 754-760
RIS	TY - JOUR T1 - Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India AU - Fateh Sıngh , Ganesh Gangaram Sonawane , Jyoti Kumar , Shivendra Kumar Dıxıt , Rajendra Kumar Meena , Bhupendra Nath Trıpathı Y1 - 2019 PY - 2019 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 754 EP - 760 VL - 43 IS - 6 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote	%0 Turkish Journal of Veterinary and Animal Sciences Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India %A Fateh Sıngh , Ganesh Gangaram Sonawane , Jyoti Kumar , Shivendra Kumar Dıxıt , Rajendra Kumar Meena , Bhupendra Nath Trıpathı %T Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India %D 2019 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 43 %N 6 %R %U
ISNAD	Sıngh, Fateh , Sonawane, Ganesh Gangaram , Kumar, Jyoti , Dıxıt, Shivendra Kumar , Meena, Rajendra Kumar , Trıpathı, Bhupendra Nath . "Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India". Turkish Journal of Veterinary and Animal Sciences 43 / 6 (Aralık 2019): 754-760 .
AMA	Sıngh F , Sonawane G , Kumar J , Dıxıt S , Meena R , Trıpathı B . Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India. Turkish Journal of Veterinary and Animal Sciences. 2019; 43(6): 754-760.
Vancouver	Sıngh F , Sonawane G , Kumar J , Dıxıt S , Meena R , Trıpathı B . Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India. Turkish Journal of Veterinary and Animal Sciences. 2019; 43(6): 754-760.
IEEE	F. Sıngh , G. Sonawane , J. Kumar , S. Dıxıt , R. Meena ve B. Trıpathı , "Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India", Turkish Journal of Veterinary and Animal Sciences, c. 43, sayı. 6, ss. 754-760, Ara. 2019