Neslihan MUTLU, Pınar AKSU KILIÇLE, Abdullah DOĞAN, Ali Nazmi Can DOĞAN, Özgür ÇELEBİ, Aliye GÜLMEZ SAĞLAM, Salih OTLU

An investigation of antibacterial effects of steroids

In this study, the effects of steroid substances such as corticosterone, progesterone, estrone, beta-sitosterol, and stigmasterolon some bacteria were investigated. In the research, standard strains of the gram-positive bacteriaBacillus subtilis(lab isolate) andStaphylococcus aureus(ATCC 6538) and the gram-negative bacteriaEscherichia coli(ATCC 25922),Pasteurella multocida(ATCC 12945),andPseudomonas aeruginosa(ATCC 9027) were used. A modified broth dilution method was applied for determining the minimalinhibitory concentration (MIC) of the steroids. Mueller Hinton broth (Becton Dickinson, 211443) was used to prepare dilutions andas a medium. At the end of the study, the MIC of corticosterone forP. multocidaand the MIC of beta-sitosterol forS. aureuswere bothdetermined as 32 μg/mL. However, other steroids showed no effects on the bacteria. These steroids did not appear to have widespreadantibacterial properties.

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