Salmonella Enteritidis predominance determined by serotyping and real-time PCR in poultry-derived food and avian isolates
This study aimed to determine Salmonella enterica subspecies enterica serovar Enteritidis (SE) presence by conventional
serotyping and SE-specific real-time PCR (SE-rPCR) in poultry-derived food and avian isolates in our laboratory Salmonella spp.
collection. Conventional serotyping indicated that 32 (8 chicken meat, 10 egg, 14 avian) out of 56 (57%) isolates were SE, whereas 8 (3
chicken meat, 2 turkey meat, 3 avian) (14%) isolates were serogroup B, 6 (1 chicken meat, 1 egg, 4 avian) (11%) were serogroup C1, 4
(3 chicken meat, 1 turkey meat) (7%) were serogroup C2-C3, 4 (3 chicken meat, 1 turkey meat) (7%) were serogroup E4, and 2 (avian)
(4%) isolates were categorized as nonserogrouped/nonserotyped. Thirty-three (8/18 chicken meat, 10/11 egg, 15/23 avian) out of 56
(59%) Salmonella isolates were positive by SE-rPCR. SE was determined as the most prevalent serotype in both of the tests regardless
of the sample type. Conventional serotyping and SE-rPCR results were in agreement in all but 1 isolate. Considerably high relative
accuracy (98%), sensitivity (100%), and specificity (96%) with almost perfect agreement between the two methods (Cohen's kappa =
0.96) indicated SE-rPCR as a reliable tool in the rapid identification of SE isolates to complement conventional serotyping.
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