Alterations in hematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to mancozed

Tatlı suda yetiştirilen gökkuşağı alabalığı mancozeb'in sublethal dozuna (1/2 LC50 =1,1 mg/l) 780 l su hacmine sahip, resirkülasyonsuz tanklarda doğal ışıklanma şartlarında, sabit su akışında (1,5 l dak-1), 9-11 °C'de, havalandırılmış ve klordan arındırılmış şebeke suyunda 24 saat aralıklarla 3 hafta maruz bırakılmıştır. Hematolojik parametrelerin tespiti için antikoagülant olarak heparin kullanılarak balıklardan yaklaşık 2 cc kan alınmıştır. Hematolojik parametreler her hafta incelenmiştir. Mevcut çalışmanın sonuçlan mancozeb'in gökkuşağı alabalığının hematolojik parametreleri üzerinde bazı etkilere sahip olduğunu göstermiştir. Pestisit kullanımının RBC (eritrosit sayısı)'de hafif bir artışa, Hb (hemoglobin), MCH (eritrosit başına düşen ortalama hemoglobin miktarı), MCHC (eritrosit başına düşen ortalama hemoglobin konsantrasyonu), PCV (hematokrit), MCV (ortalama eritrosit hacmi) ve WBC (lökosit sayısı) seviyelerinde ise düşüşe neden olduğu tespit edilmiştir. Hemoglobin muhtevası ve MCH seviyesinde pestisite maruz bırakılma esnasında önemli derecede düşüş gözlenmiştir (P < 0,05). RBC, MCHC, PCV, MCV ve WBC seviyelerinde önemli değişiklikler belirlenmemiştir (P > 0,05). RBC/WBC seviyesi deneme esnasınca WBC'deki düşüşten dolayı artmıştır.

Mancozeb'e maruz bırakılan Gökkuşağı alabalıklarının (Oncorhynchus mykiss) hematolojik parametrelerindeki değişimler

Rainbow trout reared in fresh water were exposed to a sublethal concentration of mancozeb (1/2 of LCSO =1.1 mg/l) at 24 h intervals, for 3 weeks in tanks containing 780 I water under natural light conditions with a constant flow (1.5 l min"1) of aerated dechlorinated tap water at 9-11 °C and with no recirculation. Approximately 2 cc venous blood was drawn from each subject (after weekly exposure) for the determination of hematological parameters, using heparin as an anticoagulant. Hematological parameters were investigated weekly. The findings of this study showed that mancozeb had some effect on the hematoiogical parameters of rainbow trout. It was determined that the use of the pesticide caused a slight increase in red blood cell (RBC) numbers and a decrease in hemoglobin (Hb), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), packed cell volume (PCV), mean corpuscular volume (MCV) and white blood cell (WBC) levels. Significant decreases (P < 0.05) in Hb content and in MCH were observed during exposure to the pesticide. No significant differences were observed in the levels of RBC, MCHC, PCV, MCV and WBC (P > 0.05). The RBC/WBC level increased due to the decrease in WBC during the study.

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