Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties

Glucose 6-phosphate dehydrogenase (G6PD) was purified from goose erythrocytes and some characteristics of the enzyme were investigated. The purification procedure was composed of 3 steps: hemolysate preparation, ammonium sulfate precipitation, and 2', 5'-ADP Sepharose 4B affinity gel chromatography. Thanks to the 3 consecutive procedures, the enzyme, having a specific activity of 36.2 EU/mg protein, was purified for a yield of 68.79% and 3892 folds; to ascertain enzyme purity, SDS-PAGE was performed. Optimal pH, stable pH, optimal temperature, molecular weight, and Km and Vmax values for NADP+ and glucose 6-phosphate (G6-P) substrates were also determined for the enzyme. In addition, Ki values and inhibition type were determined by means of Lineweaver-Burk graphs obtained for such inhibitors as ATP, ADP and NADPH. These materials inhibited the enzyme in a noncompetitive manner.

Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties

Glucose 6-phosphate dehydrogenase (G6PD) was purified from goose erythrocytes and some characteristics of the enzyme were investigated. The purification procedure was composed of 3 steps: hemolysate preparation, ammonium sulfate precipitation, and 2', 5'-ADP Sepharose 4B affinity gel chromatography. Thanks to the 3 consecutive procedures, the enzyme, having a specific activity of 36.2 EU/mg protein, was purified for a yield of 68.79% and 3892 folds; to ascertain enzyme purity, SDS-PAGE was performed. Optimal pH, stable pH, optimal temperature, molecular weight, and Km and Vmax values for NADP+ and glucose 6-phosphate (G6-P) substrates were also determined for the enzyme. In addition, Ki values and inhibition type were determined by means of Lineweaver-Burk graphs obtained for such inhibitors as ATP, ADP and NADPH. These materials inhibited the enzyme in a noncompetitive manner.

Kaynak Göster

Bibtex @ { tbtkveterinary151649, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2003}, volume = {27}, pages = {1179 - 1185}, doi = {}, title = {Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties}, key = {cite}, author = {Beydemir, Şükrü} }
APA Beydemir, Ş . (2003). Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties . Turkish Journal of Veterinary and Animal Sciences , 27 (5) , 1179-1185 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/12554/151649
MLA Beydemir, Ş . "Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties" . Turkish Journal of Veterinary and Animal Sciences 27 (2003 ): 1179-1185 <https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/12554/151649>
Chicago Beydemir, Ş . "Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties". Turkish Journal of Veterinary and Animal Sciences 27 (2003 ): 1179-1185
RIS TY - JOUR T1 - Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties AU - Şükrü Beydemir Y1 - 2003 PY - 2003 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 1179 EP - 1185 VL - 27 IS - 5 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties %A Şükrü Beydemir %T Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties %D 2003 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 27 %N 5 %R %U
ISNAD Beydemir, Şükrü . "Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties". Turkish Journal of Veterinary and Animal Sciences 27 / 5 (Mayıs 2003): 1179-1185 .
AMA Beydemir Ş . Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties. Turkish Journal of Veterinary and Animal Sciences. 2003; 27(5): 1179-1185.
Vancouver Beydemir Ş . Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties. Turkish Journal of Veterinary and Animal Sciences. 2003; 27(5): 1179-1185.
IEEE Ş. Beydemir , "Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties", Turkish Journal of Veterinary and Animal Sciences, c. 27, sayı. 5, ss. 1179-1185, May. 2003

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