A survey of a&atoxinM1 levels in Kahramanmaraş cheese

Çalışmada HPLC (High pressure liquid chromatography) ile 46 adet yarı sert peynir örneğinde (22 adet inek, 18 adet keçi, 6 adet koyun sütünden yapılmış) Aflatoxin M1 (AFM1) oranları belirlenmiştir. Koyun peynirlerinde AFM1 tespit edilemezken, inek ve keçi peynirlerinin 32 tanesinde 0,069-1,2 ng g -1 ve 0,06-0,22 ng g -1 oranında AFM1 tespit edilmiştir. İnek peynirlerinin analiz sonuçları % 96 oranında Türk Gıda Kodeksi peynirde AFM1 sınırı olan 0,25 ppb değerine uygunluk gösterirken 1 tanesi 1,2 ng g -1 , diğeri 0,25 ng g -1 AFM1 olarak tespit edilmiştir.

Kahramanmaraş peynirinde a&atoxinM1 oranlarının belirlenmesi üzerine bir çalışma

AflatoxinM1 (AFM1) levels weremeasured in 46 Kahramanmaraş semi-hard cheese samples (22 frombovine milk, 18 fromgoatmilk, and 6 fromovinemilk) using high-pressure liquid chromatography (HPLC). None of the ovine milk cheese samples contained AFM1. AFM1 was present in 32 samples (69.6%) of bovine and goat cheese. Bovinemilk cheese and goat milk cheese samples contained 0.069-1.2 ng g -1 and 0.06-0.22 ng g -1 of AFM1, respectively. With the exception of 2 bovine milk cheese samples (one contained 1.2 ng g -1 of AFM1, the other contained 0.25 ng g -1 of AFM1), the other samples (96%) had levels of AFM1 below the acceptable limit for cheese (0.25 ppb) set forth by the Turkish Alimentarus Codex.

___

  • 1. Applebaum, R.S., Brackett, R.E., Wiseman, D.W., Marth, E.H.: Aflatoxin: toxicity to dairy cattle and occurrence in milk and milk products. J. Food Protect., 1982; 45: 752-777.
  • 2. Sweeney, M.J., Dobson, A.D.: Mycotoxin production by Aspergillus, Fusarium and Penicillium species. Int. J. Food Microbiol., 1998; 43: 141-158.
  • 3. Ito, Y., Peterson, S.W., Wicklow, D.T., Goto, T.: Aspergillus pseudotamarii, a new aflatoxin producing species in Aspergillus section Flavi. Mycol. Res., 2001; 105: 233-239.
  • 4. Peterson, S.W., Ito, Y., Horn, B.W., Goto, T.: Aspergillus bombycis, a new aflatoxigenic species and genetic variation in its sibling species, A. nominus. Mycologia, 2001; 93: 689-703.
  • 5. Goto, T.,Wicklow, D.T., Ito, Y.: Aflatoxin and cyclopiazonic acid production by a sclerotium-producing Aspergillus tamarii strain. Appl. Environ. Microbiol., 1996; 62: 4036-4038.
  • 6. Klich,M.A.,Mullaney, E.J., Daly, C.B., Cary, J.W.:Molecular and physiological aspects of aflatoxin and sterigmatocystin biosynthesis by Aspergillus tamarii and A. ochraceoroseus. Appl. Microbiol. Biotechnol., 2000; 53: 605-609.
  • 7. Wood, G.E.: Aflatoxin M1. In: Sharma, R.P., Salunkhe, D.K. Eds., Mycotoxins and Phytoalexins. London, CRC Inc. 1991; 145-163.
  • 8. FAO/WHO News Archive: Codex Committee on Food Additives and Contaminants. Thirtienth Session, The Hague, Netherlands, 9-13 March. 1998.
  • 9. Turkish Alimentarus Codex: Microbial Toxins, Metal and Metalloid Residues. 2002; 63.
  • 10. Sharman, M., Patey, A.L., Gilbert, J.: Application of an immunoaffinity column sample clean-up to the determination of aflatoxin M1 in cheese. J. Chromatogr., 1989; 474: 457-461.
  • 11. Galvano, F., Galofaro, V., Galvano, G.: Occurrence and stability of aflatoxinM1 inmilk andmilk products: a worldwide review. J. Food Protect., 1996; 59: 1079-1090.
  • 12. Kiermeier, F.,Mashaley, R.: Influence of rawmilk processing on the aflatoxinM1 content ofmilk products. Z. Lebensm. Unters. For., 1977; 164: 183-187. (Article in German with an abstract in English)
  • 13. Zerfiridis, G.K.: Potential aflatoxin hazards to human health from direct mold growth on Teleme cheese. J. Dairy Sci., 1985; 68: 2184-2188.
  • 14. Blanco, J.L., Domínguez, L., Gómez-Lucía, E., Garayzabal, J.F.F., Goyache, J., Suárez, G.: Behavior of aflatoxin during the manufacture, ripening and storage ofManchego-type cheese. J. Food Sci., 1988; 53: 1373-1376.
  • 15. Tekinşen, K.K., Tekinşen, O.C.: Aflatoxin M1 in white pickle and Van otlu (herb) cheeses consumed in southeastern Turkey. Food Control, 2005; 16: 565-568.
  • 16. Kıvanç, M.: Mold growth and presence of aflatoxin in some Turkish cheeses. J. Food Safety, 1990; 10: 287-294.
  • 17. Gürbüz, Ü., Nizamlıoğlu, M., Nizamlıoğlu, F., Dinc¸, İ., Doğruer, Y.: Bazı et ve süt ürünleri ile baharatlarda aflatoxin aranması. Veterinarium, 1999; 10: 34-41.
  • 18. Kamkar, A.: A study on the occurrence of aflatoxin M1 in Iranian Feta cheese. Food Control, 2006; 17: 768-775.
  • 19. Sarımehmetoğlu, B., Küplülü, Ö., Çelik, T.H.: Detection of aflatoxin M1 in cheese samples by ELISA. Food Control, 2004; 15: 45-49
  • 20. Jay, J.M.: Modern Food Microbiology. New York, Champman and Hall, 1992; 1-70.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK