Kenan TÜRKYILMAZ, Süheyla TÜRKYILMAZ, Osman KAYA

A comparative study of detection of Bordetella avium antibodies in turkeys by ELISA, SPAT and AGID test

Bu çalışmanın amaçları Bordetella avium'a maruz kalmış hindilerin serolojik olarak saptanması için serum pleyt aglutinasyon test (SPAT) ve agar jel immunodiffuzyon (AGID) test antijenlerinin geliştirilmesi, ticari enzimle işaretli immun deneyi (ELISA) ile SPAT ve AGID testinin duyarlılıklarının karşılaştırılması, Aydın'da hindi sürülerinde B. avium antikorlarının serolojik olarak taranmasıdır. Bu amaçla, 300 hindiden toplanan serum örnekleri ELISA, SPAT ve AGID test yöntemleriyle incelendi. Seroprevalens ELISA, SPAT ve AGID testi için sırasıyla % 44, % 28, % 15 olarak belirlendi. çalışmada, B. avium antikorlarının tespit edilmesinde en duyarlı testin ELISA olduğu; bununla birlikte, SPAT ve AGID testinin de B. avium antikorlarının belirlenmesinde kullanılabileceği tespit edildi.

Hindilerde Bordetella avium antikorlarının ELISA, SPAT ve AGID testleri ile belirlenmesi üzerine karşılaştırmalı bir araştırma

The aims of this study were to develop a serum plate agglutination test (SPAT) antigen and agar gel immunodiffusion (AGID) test antigen for the serological detection of turkeys that have been exposed to Bordetella avium; to compare the sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with SPAT, and AGID test, and to survey B. avium antibodies in turkey flocks in Aydın, Turkey. For these purposes, serum samples collected from 300 turkeys were examined by ELISA, SPAT, and AGID test. The seroprevalence was 44%, 28%, and 15% for ELISA, SPAT, and AGID test, respectively. This study revealed that ELISA was the most sensitive test for the detection of B. avium antibodies; however, it was determined that SPAT and AGID test can also be used in screening for B. avium antibodies.

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