The Effect of Dietary Vitamin E on the Arginase Activity in the Females of Freshwater Crayfish (Astacus leptodactylus, Esch. 1823)
The effects of different levels of dietary vitamin E on the arginase activity of the hepatopancreas, gills and muscle of freshwater crayfish (Astacus leptodactylus) were investigated. The control diet contained approximately 37.41% crude protein on a dry-weight basis and 3.25 kcal g-1 gross energy. The diet ingredients contributed 65.83 mg kg-1 vitamin E to the control diet. Levels of 34.17 mg kg-1, 84.17 mg kg-1 and 134.17 mg kg-1 vitamin E (DL-a-tocopherol acetate) were added to the control diet to supply 100 mg kg-1 in diet 1, 150 mg kg-1 in diet 2 and 200 mg kg-1 in diet 3, respectively. The study was carried out in triplicate for 272 days. The results showed that arginase activities in the hepatopancreas and gills were not significantly affected by the different levels of dietary vitamin E. Vitamin E levels higher than 100 mg kg-1 caused an about 2-fold reduction in the activity of muscle arginase in the females with stage-1 juveniles in comparison with the control (P < 0.01). In the ovigerous crayfish in comparison with the control, arginase activity in the muscle was slightly increased (but not significantly) by 100 mg kg-1 vitamin E levels, and slightly decreased by 200 mg kg-1 vitamin E in diets. The presence of vitamin E higher than 150 mg kg-1 in the ovigerous crayfish and 100 mg kg-1 in the females with stage-1 juveniles in diets may negatively affect the connective tissue formation by decreasing the muscle arginase activity.
The Effect of Dietary Vitamin E on the Arginase Activity in the Females of Freshwater Crayfish (Astacus leptodactylus, Esch. 1823)
The effects of different levels of dietary vitamin E on the arginase activity of the hepatopancreas, gills and muscle of freshwater crayfish (Astacus leptodactylus) were investigated. The control diet contained approximately 37.41% crude protein on a dry-weight basis and 3.25 kcal g-1 gross energy. The diet ingredients contributed 65.83 mg kg-1 vitamin E to the control diet. Levels of 34.17 mg kg-1, 84.17 mg kg-1 and 134.17 mg kg-1 vitamin E (DL-a-tocopherol acetate) were added to the control diet to supply 100 mg kg-1 in diet 1, 150 mg kg-1 in diet 2 and 200 mg kg-1 in diet 3, respectively. The study was carried out in triplicate for 272 days. The results showed that arginase activities in the hepatopancreas and gills were not significantly affected by the different levels of dietary vitamin E. Vitamin E levels higher than 100 mg kg-1 caused an about 2-fold reduction in the activity of muscle arginase in the females with stage-1 juveniles in comparison with the control (P < 0.01). In the ovigerous crayfish in comparison with the control, arginase activity in the muscle was slightly increased (but not significantly) by 100 mg kg-1 vitamin E levels, and slightly decreased by 200 mg kg-1 vitamin E in diets. The presence of vitamin E higher than 150 mg kg-1 in the ovigerous crayfish and 100 mg kg-1 in the females with stage-1 juveniles in diets may negatively affect the connective tissue formation by decreasing the muscle arginase activity.
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- Rawn, J.D.: Catabolism of Amino Acids and the Urea Cycle. In: Foster, S. Ed. Biochemistry. 1st ed., Carolina Biological Supply Company, Burlington, North Carolina, 1989; 457-487. 2. Brown, G.J., Cohen P.P.: Comparative biochemistry of urea synthesis: 3. Activities of urea-cycle enzymes in various higher and lower vertebrates. Biochem. J., 1960; 75: 82-91. 3. Aminlari, M., Vaseghi, T.: Arginase distribution in tissues of domestic animals. Comp. Biochem. Physiol. B, 1992; 103: 385-389. 4.
- Hird, F.J., Cianciosi, S.C., McLean, R.M.: Investigations on the
- origin and metabolism of the carbon skeleton of ornithine,
- arginine and proline in selected animals. Comp. Biochem. Physiol.
- B, 1986; 83: 179-84. 5.
- Hanlon, D.P.: The distribution of arginase and urease in marine
- He, H., Lawrence, A.L.: Vitamin E requirement of Penaeus vannemei. Aquaculture, 1993; 118: 245-255.
- Park, J.R., Tappel, A.L.: Protein damage and lipid peroxidation: effects of diethyl maleate, bromotrichloromethane and vitamin E on ammonia, urea and enzymes involved in ammonia metabolism. Toxicol. Lett., 1991; 58: 29-36.
- Reigh, R.C., Braden, S.L., Craig, R.J.: Apparent digestibility coefficients for common feedstuffs in formulated diets for red swamp crayfish, Procambarus clarkii. Aquaculture, 1990; 84: 321-334.
- Miller, K.W., Lorr, N.A., Yang, C.S.: Simultaneous determination of plasma retinol α-tocopherol, lycopene, α-carotene, and β- carotene by high performance liquid chromatography. Anal. Biochem., 1984; 138: 340-345.
- AOAC: Official methods of analysis. 15th ed., Helrich, K. Ed., Association of Official Analytical Chemists, Washington, DC, 1990.
- American Public Health Association (APHA): Standard methods for the examination of water and wastewater. 16th ed., Washington, DC, 1985; 1268.
- Geyer, J.W., Dabich, D.: Rapid method for determination of arginase activity in tissue homogenates. Anal. Biochem., 1971; 39: 412-417.
- Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J.: Protein measurements with the folin phenol reagent. J. Biol. Chem., 1951; 193: 265-275.
- Chiu, Y.N., Austic, R.E., Rumsey, G.L.: Urea cycle activity and arginine formation in rainbow trout (Salmo gairdneri). J. Nutr., 1986; 116: 1640-1650.
- Cvancara, V.A.: Studies on tissue arginase and ureagenesis in fresh-water teleosts. Comp. Biochem. Physiol., 1969; 30: 489- 496.
- Vijayan, M.M., Mommsen, T.P., Glemet, H.C., Moon, T.W.: Metabolic effects of cortisol treatment in a marine teleost, the sea raven. J. Exp. Biol., 1996; 199: 1509-1514.
- Funkhouser, D., Goldstein, L., Forster, R.P.: Urea biosynthesis in the South American lungfish, Lepidosiren paradoxa: relation to its ecology. Comp. Biochem. Physiol. A, 1972; 41: 439-443.
- Janssens, P.A., Cohen P.P.: Biosynthesis of urea in the estivating African lungfish and in Xenopus laevis under conditions of water- shortage. Comp. Biochem. Physiol., 1968; 24: 887-898.
- Jurss, K., Bittorf, T., Vokler, T., Wacke, R.: Effects of temperature, food deprivation and salinity on growth, RNA/DNA ratio and certain enzyme activities in rainbow trout (Salmo gairdneri Richardson). Comp. Biochem. Physiol. B, 1987; 87: 241-253.
- Huisman, E.A.: Food conversion efficiencies at maintenance and production levels for carp, Cyprinus carpio L., and rainbow trout, Salmo gairdneri Richarson. Aquaculture, 1976; 9: 259-273.
- Marinescu, G.: Variation of the arginase activity related to the nutrition factor in the process of fish adaptation to temperature. Rev. Roum. Biol. Zool., 1973; 18: 289-294.
- Künnemann, H., Marinescu, G.: Umstellungen des Stoffwechsels des Fisches Idus idus L. (Cyprinidae) wahrend des Hungers. Verh. Dtsch. Zool. Ges., 1980; 1980: 311.
- Eriflir, M., Beytut, E., Ozan, S., Aksakal, M.: Effects of dietary vitamin E and selenium on arginase activity in the liver, kidney and heart of rats treated with high doses of glucocorticoids. Cell Biochem. Funct., 2003; 21: 331-335.
- Harlioglu, M.M., Barim, Ö.: The effect of dietary vitamin E on the pleopodal egg and stage-1 juvenile numbers of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823). Aquaculture, 2004; 236: 267-276.
- Hird, F.J., Cianciosi, S.C., McLean, R.M., Niekrash, R.E.: On the possible significance of the transamidination reaction in evolution. Comp. Biochem. Physiol. B, 1983; 76: 489-495.