İzzet KARAHAN, Gaffari TÜRK, Seyfettin GÜR

In Vitro Effects of Prostaglandin F2a and Metamizol on the Motility of Diluted Bull Semen

The purpose of the present study was to investigate in vitro effects of prostaglandin F2 alpha (PGF2a) and metamizol on the motility of diluted bull semen during short-term storage at 4 ºC. In this study, 3 healthy adult Brown-Swiss bulls, each 3 years old, were used. Semen samples were collected with an artificial vagina. Samples were taken from each bull twice a week over the course of 3 weeks (6 samples per bull). One millilitre of semen was removed from each sample for the determination of semen volume, mass activity, initial motility, sperm concentration, and abnormal sperm rate. The remainder of the semen samples of each bull were diluted to a concentration of 1:10 (semen:extender) with isotonic sodium citrate + egg yolk solution. The diluted semen samples were then divided into 12 sterile tubes of equal volume (0.5 ml). Different amounts of PGF2a [0 (PGF2a-free), 125, 250, 500, 1000, and 1500 µg] were added to 6 of these samples at 37 ºC. Similarly, different amounts of metamizol [0 (metamizol-free), 125, 250, 500, 1000, and 1500 µg] were added to the other 6 samples at 37 ºC. After the supplementation of PGF2a or metamizol, all semen samples were stored at 4 ºC. The sperm motility of the samples was examined at 0, 1, 2, 4, 24, 48, 72, 96, 120, 144, 168, 192, and 216 h. No significant differences in spermatological characteristics were determined between the bulls. Sperm supplementation with 125 µg and 250 µg PGF2a caused a statistically significant increase (P < 0.05) in the motility of diluted semen stored at 4 ºC after 24 h versus diluted PGF2a-free semen. However, the supplementation with 500 µg and 1000 µg PGF2a caused significant motility decreases after 4 h, while 1500 µg PGF2a also caused a decrease after 2 h compared to diluted PGF2a-free semen (P < 0.05). On the other hand, metamizol supplementation of 125 µg and 250 µg significantly decreased the motility of diluted semen stored at 4 ºC after 48 hand 24 h, respectively, while 500 µg, 1000 µg, and 1500 µg of metamizol reduced motility after 4 h (P < 0.05) in comparison to the diluted metamizol-free semen. In conclusion, the addition of small amounts of PGF2a to diluted bull semen samples may help to maintain sperm motility during short-term storage at 4 ºC.

Anahtar Kelimeler:

Bull, semen, PGF2a, metamizol, sperm motility

In Vitro Effects of Prostaglandin F2a and Metamizol on the Motility of Diluted Bull Semen

Keywords:

Bull, semen, PGF2a, metamizol, sperm motility,

PDF

___

Robert, A.: Antisecretory, antiulcer, cytoprotective and diarrhoegenic properties of prostaglandins. In: Samuellson, B., Paoletti, M., Eds. Advances in Prostaglandins and Thromboxane Research. 2nded., Raven Press, New York, 1976; 507.
Pineda, M.H.: Female reproductive system. In: Pineda, M.H., Dooley, M.P. Eds. McDonald’s Veterinary Endocrinology and Reproduction. 5thed., Iowa State Press, A Blackwell Publishing Company, USA, 2003; 283-340. 3. Bygdeman, M., Bendvold, E., Gottlieb, C., Svanborg, K., Eneroth, P.: Prostaglandins and male fertility. Adv. Prostaglandin Tromboxane Leukot. Res., 1985; 15: 609-611.
Foegh, M.L., Hecker, M., Ramwell, P.W.: The eicosanoids: prostaglandins, thromboxanes, leukotrienes, & related compounds. In: Greenspan, F.S. Ed. Basic and Clinical Endocrinology. 3rd Connecticut/Los Altos, California, 1991; 53-65.
Templeton, A.A., Cooper, I., Kelly, R.W.: Prostaglandin concentrations in the semen of fertile men. J. Reprod. Fertil., 1978; 52: 147-150.
Cohen, M.S., Colin, M.J., Golimbu, M., Hotchkiss, R.S.: The effects of prostaglandins on sperm motility. Fertil. Steril., 1977; 28: 78-85.
Fayed, A.H.: Effect of prostaglandin F2 alpha and methylxanthines on enzymic release of bull epididymal spermatozoa in vitro. Contraception, 1996; 53: 181-184.
Grunberger, W., Maier, U., Lunglmayr, G.: Effect of prostaglandin F2 alpha on sperm motility in vitro. Reproduction, 1981; 5: 141- 144.
Kozumplik, J., Martinek, J.: The effect of oestrophan spofa (synthetic analog of prostaglandin Fα) added to the insemination dose on pregnancy and fertility in sows. Vet. Med. (Praha), 1986; 31: 227-232.
Procaccini, J.C., Gimeno, M.F., Kesseru, E., Asch, R.H.: Human semen prostaglandins do not affect sperm motility and migration. Acta Eur. Fertil., 1985; 16: 263-266.
Gottlieb, C., Svanborg, K., Eneroth, P., Bygdeman, M.: Effect of prostaglandins on human sperm function in vitro and seminal adenosine triphosphate content. Fertil. Steril., 1988; 49: 322- 327.
Maes, D.G.D., Mateusen, B., Rijsselaere, T., De Vliegher, S., Van Soom, A., de Kruif, A.: Motility characteristics of boar spermatozoa after addition of prostaglandin F2α. Theriogenology, 2003; 60: 1435-1443.
Traas, A.M., Root Kustritz, M.V.: Effect of administrating oxytocin or prostaglandin F2αon characteristics of the canine ejaculate. Can. Vet. J., 2004; 45: 999-1002.
Kreider, J.L., Ogg, W.L., Turner, J.W.: Influence of prostaglandin F2αon sperm production and seminal characteristics of the stallion. Prostaglandins, 1981; 22: 903-913.
Pirinçci, İ., Karahan, İ., Bozkurt, T., Güler, O.: Koçlarda prostaglandin F2α, furosemid ve indometasinin sperma özellikleri üzerine etkilerinin arafltırılması. S.Ü. Vet. Bil. Derg., 2001; 17: 83-88.
Singh, S.K., Dominic, C.J.: Prostaglandin F2alpha-induced changes in the sex organs of the male laboratory mouse. Exp. Clin. Endocrinol., 1986; 88: 309-315.
Booth, N.H.: Nonnarcotic analgesices. In: Booth, N.H., McDonald, L.E. Eds. Veterinary Pharmacology and Therapeutics. Iowa State Univ. Press, Ames Iowa, 1991; 329-346.
Kennedy, J.H., Korn, N., Thurston, R.J.: Prostaglandin levels in seminal plasma and sperm extracts of the domestic turkey, and the effects of cyclooxygenase inhibitors on sperm mobility. Reprod. Biol. Endocrinol., 2003; 1: 74-80.
Knuth, U.A., Kuhne, J., Crosby, J., Bals-Pratsch, M., Kelly, R.W., Nieschlag, E.: Indomethacin and oxaprozin lower seminal prostaglandin levels but do not influence sperm motion characteristics and serum hormones of young healthy men in a placebo-controlled double-blind trial. J. Androl., 1989; 10: 108- 119.
Campos, C., de Gregoria, R., Garcia-Niceto, R., Gago, F., Ortiz, P., Alemany, S.: Regulation of cyclooxygenase activity by metamizole. Eur. J. Pharmacol., 1999; 378: 339-347.
Conte, D., Nordio, M., Romanelli, F., Manganelli, F., Giovenco, P., Dondero, F., Isidori, A.: Role of seminal prostaglandins in male fertility. II. Effects of prostaglandin synthesis inhibition on spermatogenesis in man. J. Endocrinol. Invest., 1985; 8: 289- 291.
Tanyıldızı, S., Bozkurt, T.: Effect of acetylsalicylic acid and metamizol on hyaluronidase activity and sperm characteristics in rams. Anim. Reprod. Sci., 2003; 76: 195-204.
Barth, A.D.: Evaluation of potential breeding soundness of the bull. In: Youngquist, R.S. Ed. Current Therapy in Large Animal Theriogenology. WB Saunders Company, Philadelphia, Pennsylvania. 1997; 222-236.
Bearden, H.J., Fuquay, J.W., Willard, S.T.: Applied Animal Reproduction. 6thed., Pearson Prentice Hall, Upper Saddle River, New Jersey. 2004.
Abbitt, B., Seidel, G.E. Jr., Berndtson, W.E.: Effect of tris (hydroxymethyl) aminomethane salt of prostaglandin F2alpha on post-thaw motility of bovine spermatozoa. J. Dairy Sci., 1977; 60: 1991-1993.
Didolkar, A.K., Roychowdhury, D.: Effects of prostaglandins E-1, E-2, F-1α, and F-2α, on human sperm motility. Andrologia, 1980; 12: 135-140.
Asok Kumar, R., Chinoy, N.J.: Effects of acetylsalicylic acid on reproductive organs of adolescent male rats. Endocrinol. Exp., 1988; 22: 187-195.
Çiftçi, M., Özmen, İ., Büyükokuroğlu, M.E., Pençe, S., Küfrevioğlu, Ö.İ.: Effects of metamizol and magnesium sulfate on enzyme activity of glucose 6-phosphate dehydrogenase from human erythrocyte in vitro and rat erythrocyte in vivo. Clin. Biochem., 2001; 34: 297-302.