Radoslava VLCKOVA, Jan POSIVAK, İgor VALOCKY, Zuzana KRAVCOVA, Andrea EIBENOVA, Drahomíra SOPKOVA

The effect of short-term lupin (Lupinus angustifolius) feed supplementation on serum steroid hormones, insulin-like growth factor I, and ovarian follicular development and atresia in Merino ewes

The effects of short-term lupin supplementation on ovulation rate in ewes during oestrous season are well known. In this study, we tested the effects of an 8-day lupin supplementation on folliculogenesis during anoestrus applied over the hormonal treatment of ewes. Fourteen anoestrous Merino ewes were assigned into 2 groups (n = 7 for each) in which the control group was fed a basal diet and the experimental group was supplemented with lupin groats (500 g per head per day). Both groups were treated intramuscularly with 12.5 µg/head lecirelinum (luteinising hormone releasing hormone super analogue) on the first day of supplementation and 5 days later with 37.5 µg/head cloprostenolum D (prostaglandin F2 alpha analogue). Serum sexual steroid and insulin-like growth factor I (IGF-I) concentrations were assessed using radioimmunoassay and enzyme-linked immunosorbent assay methods, respectively. Folliculogenesis was evaluated using a light microscope. Lupin feeding did not affect progesterone or IGF-I, but it significantly decreased the concentration of oestradiol-17beta. Lupin significantly increased the number of follicles of 3-5 mm in diameter and the total rate of atresia. The follicles mainly collapsed and underwent late atresia. The size of healthy and collapsed follicles increased in the lupin-fed group. These results indicated that 8-day lupin supplementation affected follicular development and atresia through an IGF-I/oestradiol feedback system.

Keywords:

Lupin feeding, ovarian follicle, atresia, oestradiol-17beta, insulin-like growth factor I,

PDF

___

Gulliver CE, Friend MA, Robertson SM, Martin GB, Clayton EH, King BJ. Short-term lupin feeding: can it decrease ovulation rate in Merino ewes? In: Proceedings of the Australian Society of Animal Production 28; 2010. p. 100.
Muñoz-Gutiérrez M, Blache D, Martin GB, Scaramuzzi RJ. Foliculogenesis and ovarian expression of mRNA encoding aromatase in anoestrous sheep after 5 days of glucose or glucosamine infusion or supplementary lupin feeding. Reproduction 2002; 124: 721–731.
Lindsay DR. Reproduction in the sheep and goat. In: Cupps PT, editor. Reproduction in Domestic Animals. 4th ed. San Diego, CA, USA: Academic Press; 1991. pp. 491–515.
Kosior-Korzecka U, Bobowiec R, Wăłjcik M. Hormonal and metabolic changes at the late phase of oestrus after stimulation of ovulation rate by lupin grain in sheep. Annales UMCS (Lublin) Sectio DD 2002; 58: 15–24.
Somchit A, Campbell BK, Khalid M, Kendall NR, Scaramuzzi RJ. The effect of short-term nutritional supplementation of ewes with lupin grain (Lupinus luteus), during the luteal phase of the estrous cycle on the number of ovarian follicles and the concentrations of hormones and glucose in plasma and follicular fluid. Theriogenology 2007; 68: 1037–1046.
Somchit-Assavacheep A. Influence of nutritional management on folliculogenesis in ewes. Thai J Vet Med Suppl 2011; 41: 25– 29.
Muñoz-Gutiérrez M, Blache D, Martin GB, Scaramuzzi RJ. Ovarian follicular expression of mRNA encoding the type I IGF receptor and IGF-binding protein-2 in sheep following five days of nutritional supplementation with glucose, glucosamine or lupins. Reproduction 2004; 128: 747–756.
Armstrong DG, McEvoy TG, Baxter G. Effect of dietary energy and protein on bovine follicular dynamics and embryo production in vitro: associations with the ovarian insulin-like growth factor system. Biol Reprod 2001; 64: 1624–1632.
Webb R, Garnsworthy PC, Gong JG, Armstrong DG. Control of follicular growth: local interactions and nutritional influences. J Anim Sci 2004; 82: 63–74.
Daftary SS, Gore AC. IGF-1 in the brain as a regulator of reproductive neuroendocrine function. Exp Biol Med 2005; 230: 292–306.
Mazerbourg S, Bondy CA, Zhou J, Monget P. The insulin-like growth factor system: a key determinant role in the growth and selection of ovarian follicles? A comparative study. Reprod Domest Anim 2003; 38: 247–258.
Williams CJ, Erickson GF. Morphology and Physiology of the Ovary. Female Reproductive Endocrinology (Endotext.org). MdText.com Inc.: South Dartmouth, MA, USA; 2003.
Sirotkin AV. Regulators of Ovarian Functions. 1st ed. New York, NY, USA: Nova Science; 2010.
Marion GB, Gier HT, Choudary JB. Micromorphology of the bovine ovarian follicular system. J Anim Sci 1968; 27: 451–465.
Vlčková R, Valocký I, Lazar G, Sopková D, Maraček I. Histological and ultrasonographic monitoring of folliculogenesis in puerperal ewes after spring lambing. Acta Vet Brno 2008; 77: 65–72.
Vacek Z. Histologická technika. In: Vacek Z, editor. Histológia a Histologická Technika. Martin, Slovakia: Vydavateľstvo Osveta; 1990, pp. 267–488 (in Slovak).
Scaramuzzi RJ, Baird DT, Campbell BK, Driancourt MA, Dupont J, Fortune JE, Gilchrist JB, Martin GB, McNatty KP, McNeilly AS et al. Regulation of folliculogenesis and the determination of ovulation rate in ruminants. Reprod Fertil Dev 2011; 23: 444– 467.
Viñoles C, Paganoni BL, McNatty KP, Heath DA, Thompson AN, Glover KM, Milton JT, Martin GB. Follicle development, endocrine profiles and ovulation rate in adult Merino ewes: effects of early nutrition (pre- and post-natal) and supplementation with lupin grain, Reproduction 2014; 147: 101–110.
Estívariz FC, Ziegler TR. Nutrition and the insulin-like growth factor system. Endocrine 1997; 7: 65–71.
Campbell BK. Factors affecting ovulation rate in sheep and cattle. PhD, University of Sydney, Sydney, Australia, 1988.
Kravcová Z, Sopková D, Vlčková R, Ondrašovičová S, Danko J. Electroforetické stanovenie hladín cholesterolu v sére bahníc po skrmovaní lupiny şzkolistej. In: Zborník zo Seminára doktorandov v Košiciach. Košice, Slovakia: SAV; 2011. pp. 75–78 (in Slovak).