Kürşat Bora ÇARMAN, Yasemin KARAL, Gül Gülen MERT, Arzu EKİCİ, Peren PERK, Didem ARSLANTAŞ, Coskun YARAR, Ener Çağrı DİNLEYİCİ
Expression of MicroRNA 146a, 155, 181 and 223 in febrile seizure
Background. We studied microRNAs (miRNAs) -146a, -155, -181 and -223 expressions and proinflammatory cytokine levels in children with Febrile seizure (FS) and compared to febrile controls. Methods. This prospective multicenter study examined representative populations in eight different cities in Turkey between June 30, 2018 and July 1, 2019. Blood samples were taken from all children at presentation. The real time (RT) polymerase chain reaction (PCR) were used to measure the expressions of microRNAs and tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) levels were studied by enzyme-linked immuno-sorbent assay. Results. The study was conducted with 60 children; 30 children with FS and 30 children in the febrile control group. The seizure was classified as simple FS in 73.3 % and half of the children were experiencing their first FS episode. Although the expression levels of miRNAs-146a, -181a and -155 were higher in febrile seizure patients, only miRNAs 146a level was significantly higher in FS patients. Serum TNF-α, IL-1β, IL-6 levels were higher in the FS group than the controls. The results of statistical analysis showed that there were correlations within miRNA expressions in children with FS. No differences were found considering miRNA expression between FS type, number of FS experienced. Conclusions. miRNAs-146a, -181a, -155 and -223 may be involved in FS pathogenesis. Altered miRNA expression levels might be an adaptive response to inflammation. New therapeutic approaches might be developed based on miRNA expressions in children with FS.
___
1. Patterson JL, Carapetian SA, Hageman JR, Kelley KR. Febrile seizures. Pediatr Ann 2013; 42: 249‐254.2. Kwon A, Kwak BO, Kim K, et al. Cytokine levels in febrile seizure patients: a systematic review and meta- analysis. Seizure 2018; 59: 5-10.
3. Bushati N, Cohen SM. microRNA functions. Annu Rev Cell Dev Biol 2007; 23: 175-205.
4. Lovisari F, Simonato M. Gene networks and microRNAs: promises and challenges for treating epilepsies and their comorbidities. Epilepsy Behav 2019: 106488. doi:10.1016/j.yebeh.2019.106488.
5. Aronica E, Fluiter K, Iyer A, et al. Expression pattern of miR-146a, an inflammation-associated microRNA, in experimental and human temporal lobe epilepsy. Eur J Neurosci 2010; 31: 1100-1107.
6. Srivastava A, Dixit AB, Banerjee J, Tripathi M, Sarat Chandra P. Role of inflammation and its miRNA based regulation in epilepsy: implications for therapy. Clin Chim Acta 2016; 452: 1-9.
7. Omran A, Peng J, Zhang C, et al. Interleukin-1β and microRNA-146a in an immature rat model and children with mesial temporal lobe epilepsy. Epilepsia 2012; 53: 1215-1224.
8. Ashhab MU, Omran A, Kong H, et al. Expressions of tumor necrosis factor alpha and microRNA-155 in immature rat model of status epilepticus and children with mesial temporal lobe epilepsy. J Mol Neurosci 2013; 51: 950-958.
9. Carman KB, Calik M, Karal Y, et al. Viral etiological causes of febrile seizures for respiratory pathogens (EFES Study). Hum Vaccin Immunother 2019; 15: 496-502.
10. Auvinen E. Diagnostic and prognostic value of microRNA in viral diseases. Mol Diagn Ther 2017; 21: 45-57.
11. Tahamtan A, Inchley CS, Marzban M, et al. The role of microRNAs in respiratory viral infection: friend or foe? Rev Med Virol 2016; 26: 389-407.
12. Issac MS, Girgis M, Haroun M, Shalaby A. Association of genetic polymorphism of pre-microRNA-146a rs2910164 and serum high-mobility group box 1 with febrile seizures in Egyptian children. J Child Neurol 2015; 30: 437–-444.
13. Wang B, Cao X, Lin J, Qian Q, Yu L, Qian Q. Upregulation of microRNA-223 inhibits brain injury and hippocampal neuron apoptosis of rats after febrile seizure through the NLRP3-Caspase-1 signaling pathway. Biomed Pharmacother 2019; 114: 108683.
14. Shinnar S, Glauser TA. Febrile seizures. J Child Neurol 2002; 17(Suppl 1): S44-S52.
15. Willems E, Leyns L, Vandesompele J. Standardization of real-time PCR gene expression data from independent biological eplicates. Anal Biochem 2008; 379: 127-129.
16. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001; 25: 402-408.
17. Chu C, Lei X, Li Y, et al. High expression of miR- 222-3p in children with Mycoplasma pneumoniae pneumonia. Ital J Pediatr 2019; 45: 163.
18. Cui J, Zhou B, Ross SA, Zempleni J. Nutrition, microRNAs, and human health. Adv Nutr 2017; 8: 105-112.
19. Hayes J, Peruzzi PP, Lawler S. MicroRNAs in cancer: biomarkers, functions and therapy. Trends Mol Med 2014; 20: 460-469.
20. Anitha A, Thanseem I. microRNA and autism. Adv Exp Med Biol 2015; 888: 71-83.
21. Huang Y, Guo J, Wang Q, Chen Y. MicroRNA-132 silencing decreases the spontaneous recurrent seizures. Int J Clin Exp Med 2014; 7: 1639-1649.
22. Reschke CR, Henshall DC. microRNA and epilepsy. Adv Exp Med Biol 2015; 888: 41-70.
23. Kim SH, Yun SW, Kim HR, Chae SA. Exosomal microRNA expression profiles of cerebrospinal fluid in febrile seizure patients. Seizure 2020; 81: 47-52.
24. Asadi-Pooya AA, Nei M, Rostami C, Sperling MR. Mesial temporal lobe epilepsy with childhood febrile seizure. Acta Neurol Scand 2017; 135: 88-91.
25. Ren L, Zhu R, Li X. Silencing miR-181a produces neuroprotection against hippocampus neuron cell apoptosis post-status epilepticus in a rat model and in children with temporal lobe epilepsy. Genet Mol Res 2016; 15. doi: 10.4238/gmr.15017798.
26. Tao H, Zhao J, Liu T, et al. Intranasal delivery of miR- 146a mimics delayed seizure onset in the lithiumpilocarpine mouse model. Mediators Inflamm 2017; 2017: 6512620.
27. Huang Y, Liu X, Liao Y, et al. MiR-181a influences the cognitive function of epileptic rats induced by pentylenetetrazol. Int J Clin Exp Pathol 2015; 8: 12861-12868.
28. Trobaugh DW, Klimstra WB. MicroRNA regulation of RNA virus replication and pathogenesis. Trends Mol Med 2017; 23: 80-93.
29. Qi Y, Tu J, Cui L, et al. High-throughput sequencing of microRNAs in adenovirus type 3 infected human laryngeal epithelial cells. J Biomed Biotechnol 2010; 2010: 915980.
30. Song H, Wang Q, Guo Y, et al. Microarray analysis of microRNA expression in peripheral blood mononuclear cells of critically ill patients with influenza A (H1N1). BMC Infect Dis 2013; 13: 257.
31. Huang L, Ma J, Sun Y, et al. Altered splenic miRNA expression profile in H1N1 swine influenza. Arch Virol 2015; 160: 979-985.
- ISSN: 0041-4301
- Yayın Aralığı: Yılda 6 Sayı
- Başlangıç: 1958
- Yayıncı: Hacettepe Üniversitesi Çocuk Sağlığı Enstitüsü Müdürlüğü
Sayıdaki Diğer Makaleler
Intranasal supernumerary tooth in a child: a case report
Yoon Seok CHOI, Yong-Dae KİM, Chang Hoon BAE, Hyung Gyun NA
A very rare cause of protein losing enteropathy: Gaucher disease
Mehmet Akif GÖKTAŞ, Ersin GÜMÜŞ, Hülya DEMİR, Hayriye HIZIRCIOĞLU GÜLŞEN, İnci Nur Saltık TEMİZEL, Hasan ÖZEN, Aysel YÜCE, Şafak GÜÇER
Treatment results of modified BFM protocol in pediatric high-risk Burkitt lymphoma
Sema VURAL, Dildar Bahar GENÇ, Serap KARAMAN, Öner DOĞAN, Rejin KEBUDİ
Clinical profile and long-term outcome of the first seizures in children
Hakan SALMAN, Pınar ARICAN, Nihal Olgaç DÜNDAR
Hayrunnisa BEkİS BOZKURT, Yavuz ŞAHİN
Dattatray KULKARNİ, Srinivas MURKİ, Dinesh PAWALE, Soumya JENA, Deepak SHARMA, Venkateshwarlu VARDHELLİ, Venkat KALLEM, Tanveer BASHİR, Vidhyadhara NAİK, Sai KİRAN
Developmental evaluation in children experiencing febrile convulsions
Didem DERİCİ YILDIRIM, Rojan İPEK, Khatuna MAKHAROBLİDZE, Burçin GÖNÜLLÜ POLAT, Meltem ÇOBANOĞULLARI DİREK, Mustafa KÖMÜR, Çetin OKUYAZ
Severe isolated sulfide oxidase deficiency with a novel mutation
Meriç ERGENE, Nuriye YARAR, Elif Perihan ÖNCEL, Büşranur ÇAVDARLI, İsmail Zafer ECEVİT, Taner SEZER, Halil İbrahim AYDIN
Lijalem Melie TESFAW, Muluwerk Ayele DEREBE, Haile Mekonnen FENTA