Zülal Aşçı TORAMAN, Yaşar ŞEN, Şükran ÖZDİLLER, Ahmet GÖDEKMERDAN, Yasemin BULUT

Use of Nasal Samples and Genom Amplification Methods for Detection of Respiratory Viruses in Infants with Acute Lower Respiratory Tract Infection

Aim: The aim of this study was to evaluate the use of genome amplification methods [polymerase chain reaction (PCR) and reverse transcription (RT)-PCR] for detection of common respiratory viruses (RSV: respiratory syncytial virus, PIV3: parainfluenza virus 3, IVA: influenza virus type A, IVB: influenza virus type B and adenovirus) in nasal wash specimens of infants with acute lower respiratory tract infection (ALRI). Materials and Methods: The nasal and serum samples taken from 90 infants with ALRI were analyzed by genome amplification methods and ELISA. Results: In ELISA, specific IgM to only one virus and to multiple viruses was present in 39 (43.3%) and 7 (7.7%) of the serum samples, respectively. Therefore, IgM positivity to at least one virus was detected in 46 (51.1%) of the serum samples. In the genome amplification methods in nasal samples, 62 (68.8%) of these samples were positive for only one virus, whereas 9 (10%) of the samples were found to be positive for multiple viruses. Therefore, a total of 71 (78.8%) samples were accepted as positive with at least one virus. Conclusions: As a result, we recommend use of the genome amplification methods in nasal wash specimens for diagnosis of the respiratory viruses in ALRI infants.

Anahtar Kelimeler:

Acute lower respiratory tract infection, ALRI, infant, PCR, respiratory viruses

Use of Nasal Samples and Genom Amplification Methods for Detection of Respiratory Viruses in Infants with Acute Lower Respiratory Tract Infection

Keywords:

Acute lower respiratory tract infection, ALRI, infant, PCR, respiratory viruses,

PDF

___

Swierkosz EM, Erdman DD, Bonnot T, Schneiderheinze C, Waner JL. Isolation and characterization of a naturally occurring parain- fluenza 3 virus variant. J Clin Microbiol 1995; 33: 1839-1841.
Zhang W, Evans DH. PCR detection and differentiation of influen- za virus A, B, and C. In: Persing DH, Smith TF, Tenover FC, White TJ, editors. Diagnostic molecular microbiology: principles and applications. Mayo Foundation; 1993. pp. 374-82.
McDonough M, Kew O, Hierholzer J. PCR detection of human adenoviruses. In: Persing DH, Smith TF, Tenover FC, White TJ, editors. Diagnostic molecular microbiology: principles and applica- tions. Mayo Foundation; 1993. pp. 389-93.
SPSS for Windows, Release; 2000; 11.5.
Welliver RC. Review of epidemiology and clinical risk factors for severe respiratory syncytial virus (RSV) infection. Pediatr 2003; 143: 112-117.
Shaw MW, Xu X, Li Y, Normand S, Ueki RT, Kunimoto GY et al. Reappearance and global spread of variants of influenza B/Victo- ria/2/87 lineage viruses in the 2000-2001 and 2001-2002 sea- sons. Virology 2002; 303: 1-8.
Guney C, Kubar A, Yapar M, Besirbellioglu AB, Doganci L. An out- break of respiratory infection due to respiratory syncytial virus subgroup B in Ankara, Turkey. Jpn J Infect Dis 2004; 57: 178- 180.
Stensballe LG, Trautner S, Kofoed PE, Nante E, Hedegaard K, Jensen IP et al. Comparison of nasopharyngeal aspirate and nasal swab specimens for detection of respiratory syncytial virus in dif- ferent settings in a developing country. Trop Med Int Health 2002; 7: 317-321.
Englund JA, Piedra PA, Jewell A, Patel K, Baxter BB, Whimbey E. Rapid diagnosis of respiratory syncytial virus infections in immunocompromised adults. Clin Microbiol 1996; 34: 1649- 1653.
Covalciuc KA, Webb KH, Carlson CA. Comparison of four clinical specimen types for detection of influenza A and B viruses by opti- cal immunoassay (FLU OIA test) and cell culture methods. J Clin Microbiol 1999; 37: 3971-3974.