Nurten KARA, Hasan BAĞCI, Sezgin Özgür GÜNEŞ

Orta karadeniz bölgesi sağlıklı Türk populasyonunda CCRSdelta32 frekansı

Aim: Delta32 polymorphism in the chemokine receptor 5 (CCR5) gene affects human immune deficiency virus type 1 (HIV-1) entry, transmission, and outcome. The aim of this study was to determine the allelic frequencies of genetic variant CCR5delta32, which influence susceptibility to HIV-1 infection, in a northern Turkish population. Materials and Methods: We determined the allelic frequencies of CCR5delta32 in 449 unrelated healthy individuals from the central Black Sea coastal region without any known history of HIV-1 infection. The polymorphism was analyzed using polymerase chain reaction (PCR). The population was in the Hardy- Weinberg equilibrium. Delta32 allelic frequencies were calculated. Results: The frequency of mutant delta32 allele in this population was 5.2%. Conclusions: Our data indicate that the low frequency of CCR5delta32 allele may be related to higher genetic susceptibility to HIV-1 infection in the central Black Sea coastal r

The frequency of CCRSdelta32 polymorphism in the central black sea coastal region in a healthy population

PDF

___

1. Liu R, Paxton WA, Choe S, Ceradini D, Martin SR, Horuk R et al. Homozygous defect in HIV-1 co-receptor accounts for resistance of some multiply exposed individuals to HIV-1 infection. Cell 1996; 86: 367-377.
2. Dean M, Carrington M, Winkler C, Huttley GA, Smith MV, Allikmets R et al. Genetic restriction of HIV-1 infection and progression to AIDS by a deletion allele of the CCR5 structural gene. Science 1996; 273: 1856-1861.
3. Samson M, Libert F, Doranz BJ, Rucker J, Liesnard C, Farber CM et al. Resistance to HIV-1 infection in Caucasian individuals bearing mutant alleles of the CCR-5 chemokine receptor gene. Nature 1996; 382: 722-725.
4. Dean M, Carrington M, O’Brien SJ. Balanced polymorphism selected by genetic versus infectious human disease. Annu Rev Genomics Hum Genet 2002; 3: 263-292.
5. Available from: URL: http://www.eurohiv.org
6. Miller SA, Dykes DD. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res 1988; 16:1215
7. Fischereder M, Luckow B, Hocher B, Wuthrich RP, Rothenpieler U, Schneeberger H et al. CC chemokine receptor 5 and renaltransplant survival. Lancet 2001; 357: 1758-1761.
8. Libert F, Cochaux P, Beckman G, Samson M, Aksenova M, Cao A et al. The Δccr5 mutation conferring protection against HIV-1 in Caucasian populations have a single and recent origin in Northeastern Europe. Hum Mol Genet 1998; 7: 399-406.
9. Degerli N, Yilmaz E, Bardakci F. The Δ32 allele distribution of the CCR5 gene and its relationship with certain cancers in a Turkish population. Clin Biochem 2005; 38(3): 248-252.
10. Yigit B, Bozkurt N, Berber I, Titiz I, Isbir T. Analysis of CC chemokine receptor 5 and 2 polymorphisms and renal transplant survival. Cell Biochem Funct 2006; (in press).
11. Lucotte G. Frequencies of 32 base pair deletion of the (Δ32) allele of the CCR5 HIV-1 co-receptor gene in Caucasians: a comparative analysis. Infect Genet Evol 2002; 1(3): 201-205.