Urease inhibitory potential of Zizyphus oxyphylla Edgew. extracts and isolated compounds
To determine the urease inhibitory potential of extracts and isolated compounds from Zizyphus oxyphylla Edgew. Materials and methods: Methanolic extracts and subsequent fractions of Z. oxyphylla stem and 3 isolated compounds from the roots of Z. oxyphylla were obtained through column chromatography and subjected to in vitro urease inhibition. Results: Three fractions (n-hexane, ethyl acetate, and butanol fractions) showed good to excellent activity, while the chloroform fraction showed nonsignificant activity. Amongst the isolated compounds, oxyphylline D (1) was the most active of the 3 isolated cyclopeptide alkaloids, followed by nummularin R (3) and nummularin C (2). Conclusion: Z. oxyphylla has the potential to be used in the treatment of various complications produced by urease enzymes, such as gastric ulcers.
Urease inhibitory potential of Zizyphus oxyphylla Edgew. extracts and isolated compounds
To determine the urease inhibitory potential of extracts and isolated compounds from Zizyphus oxyphylla Edgew. Materials and methods: Methanolic extracts and subsequent fractions of Z. oxyphylla stem and 3 isolated compounds from the roots of Z. oxyphylla were obtained through column chromatography and subjected to in vitro urease inhibition. Results: Three fractions (n-hexane, ethyl acetate, and butanol fractions) showed good to excellent activity, while the chloroform fraction showed nonsignificant activity. Amongst the isolated compounds, oxyphylline D (1) was the most active of the 3 isolated cyclopeptide alkaloids, followed by nummularin R (3) and nummularin C (2). Conclusion: Z. oxyphylla has the potential to be used in the treatment of various complications produced by urease enzymes, such as gastric ulcers.
___
- Ashiralieva A, Kleiner D. Polyhalogenated benzo- and naphthoquinones are potent inhibitors of plant and bacterial ureases. FEBS Lett 2003; 555: 367–370.
- Demir M, Ataseven H. The effects of sequential treatment as a first-line therapy for Helicobacter pylori eradication. Turk J Med Sci 2011; 41: 427–433.
- Griffith D, Musher D, Itin C. Urease, the primary cause of infection-induced urinary stones. Invest Urol 1976; 13: 346.
- Zia-Ul-Haq M, Riaz M, DeFeo V. Ipomea hederacea Jacq.: a medicinal herb with promising health benefits. Molecules 2012; 11: 13132–45.
- Zia-Ul-Haq M, Ahmad M, Jehan N, Ahmad S, Qayum M, Marwat IK. Antimicrobial screening of selected flora of Pakistan. Arch Bio Sci 2011; 63: 691–5.
- Adzu B, Amos S, Wambebe C, Gamaniel K. Antinociceptive activity of Zizyphus spina-christi root bark extract. Fitoterapia 2001; 72: 344–50.
- Borgi W, Ghedira K, Chouchane N. Antiinflammatory and analgesic activities of Zizyphus lotus root barks. Fitoterapia 2007; 78: 16–9.
- Al-Reza SM, Yoon JI, Kim HJ, Kim JS, Kang SC. Antiinflammatory activity of seed essential oil from Zizyphus jujuba. Food Chem Toxicol 2010; 48: 639–43.
- Nisar M, Adzu B, Inamullah K, Bashir A, Ihsan A, Gilani AH. Antinociceptive and antipyretic activities of the Zizyphus oxyphylla Edgew. leaves. Phyto Res 2007; 21: 693–5.
- Borgi W, Chouchane N. Anti-spasmodic effects of Zizyphus lotus (L.) Desf. extracts on isolated rat duodenum. J Ethnopharmacol 2009; 126: 571–3.
- Shen X, Tang Y, Yang R, Yu L, Fang T, Duan JA. The protective effect of Zizyphus jujube fruit on carbon tetrachlorideinduced hepatic injury in mice by anti-oxidative activities. J Ethnopharmacol 2009; 122: 555–60.
- Michel CG, Nesseem DI, Ismail MF. Anti-diabetic activity and stability study of the formulated leaf extract of Zizyphus spinachristi (L.) Willd with the influence of seasonal variation. J Ethnopharmacol 2011; 133: 53–62.
- Li J, Liu Y, Fan L, Ai L, Shan L. Antioxidant activities of polysaccharides from the fruiting bodies of Zizyphus jujuba cv. Jinsixiaozao. Carbohy Poly 2011; 84: 390–4.
- Wahida B, Abderrahman B, Nabil C. Antiulcerogenic activity of Zizyphus lotus (L.) extracts. J Ethnopharmacol 2007; 112: 228–31.
- Nisar M, Kaleem W, Adhikari A, Ali Z, Hussain N, Khan I, Qayum M, Choudhary MI. Stereochemistry and NMR data assignment of cyclopeptide alkaloids from Zizyphus oxyphylla. Nat Prod Comm 2010; 5: 1205–8.
- Nisar M, Kaleem WA, Qayum M, Hussain A, Zia-Ul-Haq M, Ali I, Choudhary MI. Biological screening of Zizyphus oxyphylla Edgew leaves. Pak J Bot 2010; 42: 4063–9.
- Nisar M, Kaleem WA, Qayum M, Marwat IK, Zia-UL-Haq M, Ali I, Choudhary MI. Biological screening of Zizyphus oxyphylla Edgew stem. Pak J Bot 2011; 43: 311–7.
- Kaleem WA, Nisar M, Qayum M, Zia-Ul-Haq M, Adhikari A, De Feo V. New 14-membered cyclopeptide alkaloids from Zizyphus oxyphylla Edgew. Int J Mol Sci 2012; 13: 11520–9.
- Zia-Ul-Haq M, Ahmed S, Rizwani GH, Qayum M, Ahmad S, Hanif M. Platelet aggregation inhibition activity of selected flora of Pakistan. Pak J Pharm Sci 2012; 25: 863–5.
- Khan I, Ali S, Hameed S, Rama NH, Hussain MT, Wadood A et al. Synthesis, antioxidant activities and urease inhibition of some new 1,2,4-triazole and 1,3,4-thiadiazole derivatives. Euro J Med Chem 2010; 45: 5200-5207.
- Bor Z, Arslan R, Bektaş N, Pirildar S, Donmez AA. Antinociceptive, anti-inflammatory, and antioxidant activities of the ethanol extract of Crataegus orientalis leaves. Turk J Med Sci 2012; 42: 315–24.
- Motaharinia Y, Rezaee MA, Hazhir MS, Zandi F, Shapouri R, Hakhamaneshi MS et al. Evaluation of the antibacterial activity of Zataria multiflora Boiss., Rhus coriaria L., Mentha piperita L., and Ocimum basilicum L. extracts on Brucella strains isolated from brucellosis patients. Turk J Med Sci 2012; 42: 816–22.
- Bahadır Ö, Saltan HG, Özbek H. Antinociceptive activity of some Scorzonera L. species. Turk J Med Sci 2012; 42: 861–6.
- Ozturk, S., Ercisli, S. The chemical composition of essential oil and in vitro antibacterial activities of essential oil and methanol extract of Ziziphora persica Bunge. J Ethnopharmacol 2006; 106: 372–6.
- Zia-Ul-Haq M, Shahid SA, Ahmad S, Qayum M, Khan I. Antimalarial, antiemetic and antidiabetic potential of Grewia asiatica L. leaves. J Med Plants Res 2012; 6: 3213–6.
- Ercisli S, Esitken A. Fruit characteristics of native rose hip (Rosa spp.) selections from the Erzurum Province of Turkey. New Zeal J Crop Hort 2004; 32: 51–3.
- Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 2009; 22: 102–6.
- Tosun M, Ercisli S, Sengul M, Ozer H, Polat T, Ozturk E. Antioxidant properties and total phenolic content of eight Salvia species from Turkey. Biol Res 2009; 42: 175–81.