Comparison of the protective effects of desflurane and propofol anesthesia in rats: a hepatic ischemia-reperfusion injury model

It is known that desflurane and propofol protect liver tissue via different mechanisms. The aim of this study was to compare the protective effects of these 2 agents against hepatic ischemia-reperfusion injury. Materials and methods: Rats were randomly divided into 2 groups: ischemia and ischemia-reperfusion. Each group was further divided into 3 subgroups: ketamine, desflurane, and propofol. Thirty minutes after anesthetics were administered, the rats were subjected to 45 min of hepatic ischemia and 4 h of reperfusion. Blood samples and liver tissues were obtained in order to assess serum tumor necrosis factor alpha (TNF-a), interleukin-1b (IL-1b), and malondialdehyde levels and for histologic examination. Results: The postischemic serum IL-1b levels were significantly higher with propofol than with ketamine (P = 0.014). In the postischemic period, in the ischemia-desflurane group, TNF-a levels were significantly lower than in the ischemia-propofol (P = 0.009). The number of polymorphonuclear leukocytes was the lowest in the ketamine group in the postischemic period (P < 0.01). Conclusion: In order to reach a definitive judgment, studies with a larger number of subjects are necessary.

Comparison of the protective effects of desflurane and propofol anesthesia in rats: a hepatic ischemia-reperfusion injury model

It is known that desflurane and propofol protect liver tissue via different mechanisms. The aim of this study was to compare the protective effects of these 2 agents against hepatic ischemia-reperfusion injury. Materials and methods: Rats were randomly divided into 2 groups: ischemia and ischemia-reperfusion. Each group was further divided into 3 subgroups: ketamine, desflurane, and propofol. Thirty minutes after anesthetics were administered, the rats were subjected to 45 min of hepatic ischemia and 4 h of reperfusion. Blood samples and liver tissues were obtained in order to assess serum tumor necrosis factor alpha (TNF-a), interleukin-1b (IL-1b), and malondialdehyde levels and for histologic examination. Results: The postischemic serum IL-1b levels were significantly higher with propofol than with ketamine (P = 0.014). In the postischemic period, in the ischemia-desflurane group, TNF-a levels were significantly lower than in the ischemia-propofol (P = 0.009). The number of polymorphonuclear leukocytes was the lowest in the ketamine group in the postischemic period (P < 0.01). Conclusion: In order to reach a definitive judgment, studies with a larger number of subjects are necessary.

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  • Xu SQ, Li YH, Hu SH, Chen K, Dong LY. Effects of Wy14643 on hepatic ischemia reperfusion injury in rats. World J Gastroenterol 2008; 14: 6936–42.
  • Montalvo-Jave EE, Escalante-Tattersfield E, Ortega-Salgado JA, Pina E, Geller DA. Factors in the pathophysiology of the liver ischemia-reperfusion injury. J Surg Res 2008; 147: 153–9.
  • Toledo-Pareyra LH, Suzuki S. Neutrophils, cytokines, and adhesion molecules in hepatic ischemia and reperfusion injury. J Am Coll Surg 1994; 179: 758–62.
  • Jaeschke H. Mechanisms of liver injury. II. Mechanisms of neutrophil-induced liver cell injury during hepatic ischemiareperfusion and other acute inflammatory conditions. Am J Physiol Gastrointest Liver Physiol 2006; 290: G1083–8.
  • Arslan M, Kurtipek O, Dogan AT, Unal Y, Kizil Y, Nurlu N et al. Comparison of effects of anesthesia with desflurane and enflurane on liver function. Singapore Med J 2009; 50: 73–7.
  • Li Y, Zhang X, Zhu B, Xue Z. Desflurane preconditioning inhibits endothelial nuclear factor-K-B activation by targeting the proximal end of tumor necrosis factor-α signaling. Anesth Analg 2008; 106: 1473–9.
  • Musacchio E, Rizzoli V, Bianchi M, Bindoli A, Galzigna L. Antioxidant action of propofol on liver microsomes, mitochondria and brain synaptosomes in the rat. Pharmacol Toxicol 1991; 69: 75–7.
  • Laviolle B, Basquin C, Aguillon D, Compagnon P, Morel I, Turmel V et al. Effect of an anesthesia with propofol compared with desflurane on free radical production and liver function after partial hepatectomy. Fundam Clin Pharmacol 2012; 26: 735–42.
  • Demircioğlu Rİ, Usta B, Sert H, Muslu B, Gökdemir M. Taurine is protective against oxidative stress during cold ischemia in the rat kidney. Turk J Med Sci 2011; 41: 843–9.
  • Teoh N, Field J, Sutton J, Farrell G. Dual role of tumor necrosis factor-α in hepatic ischemia-reperfusion injury: studies in tumor necrosis factor-α gene knockout mice. Hepatology 2004; 39: 412–21.
  • Mosher B, Dean R, Harkema J, Remick D, Palma J, Crockett E. Inhibition of Kupffer cells reduced CXC chemokine production and liver injury. J Surg Res 2001; 99: 201–10.
  • Bedirli A, Gökahmetoğlu S, Küçük KC, Soyuer I, Güler İ, Şakrak Ö. Bacterial translocation after partial hepatic resection under ischemia and reperfusion in rats: incidence and time course. Turk J Med Sci 2003; 33: 135–40.
  • Colletti LM, Remick DG, Burtch GD, Kunkel SL, Strieter RM, Campbell DA Jr. Role of tumor necrosis factor-alpha in the pathophysiologic alterations after hepatic ischemia/ reperfusion injury in the rat. J Clin Invest 1990; 85: 1936–43.
  • Hoffmann MW, Wonigeit K, Steinhoff G, Herzbeck H, Flad HD, Pichlmayr R. Production of cytokines (TNF-alpha, IL-1beta) and endothelial cell activation in human liver allograft rejection. Transplantation 1993; 55: 329–35.
  • Yang YL, Li PJ, Xu XP, Dou KF, Yue SQ, Li KZ. Protective effects of tumor necrosis factor alpha antibody and ulinastatin on liver reperfusion in rats. World J Gastroenterol 2004; 10: 3161–4.
  • Shirasugi N, Wakabayashi G, Shimazu M, Oshima A, Shito M, Kawachi S et al. Up-regulation of oxygen-derived free radicals by interleukin-1 in hepatic ischemia/reperfusion injury. Transplantation 1997; 64: 1398–403.
  • Shito M, Wakabayashi G, Ueda M, Shimazu M, Shirasugi N, Endo M et al. Interleukin 1 receptor blockade reduces tumor necrosis factor production, tissue injury, and mortality after hepatic ischemia-reperfusion in the rat. Transplantation 1997; 63: 143–8.
  • Ko JS, Gwak MS, Choi SJ, Kim GS, Kim JA, Yang M et al. The effects of desflurane and propofol-remifentanil on postoperative hepatic and renal functions after right hepatectomy in liver donors. Liver Transpl 2008; 14: 1150–8.
  • Toller WG, Gross ER, Kertsen JR, Pagel PS, Gross GJ, Warltier DC. Sarcolemmal and mitochondrial adenosine triphosphatedependent potassium channels: mechanism of desfluraneinduced cardioprotection. Anesthesiology 2000; 92: 1731–9.
  • Biao Z, Zhanggang X, Hao J, Changhong M, Jing C. The in vitro effect of desflurane preconditioning on endothelial adhesion molecules and mRNA expression. Anesth Analg 2005; 100: 1007–13.
  • Gülçin I, Alici HA, Cesur M. Determination of in vitro antioxidant and radical scavenging activities of propofol. Chem Pharm Bull 2004; 53: 281–5.
  • Li W, Zhang Y, Liu Y, Yue F, Lu Y, Qui H et al. In vitro kinetic evaluation of the free radical scavenging ability of propofol. Anesthesiology 2012; 116: 1258–66.
  • Huang Y, Zitta K, Bein B, Scholz J, Steinfath M, Albrecht M. Effect of propofol on hypoxia re-oxygenation induced neuronal cell damage in vitro. Anaesthesia 2013; 68: 31–9.
  • Runzer TD, Ansley DM, Godin DV, Chambers GK. Tissue antioxidant capacity during anesthesia: propofol enhances in vivo red cell and tissue antioxidant capacity in a rat model. Anesth Analg 2002; 94: 89–93.
  • Murphy PG, Bennett JR, Myers DS, Davies MJ, Jones JG. The effect of propofol anesthesia on free radical-induced lipid peroxidation in rat liver microsomes. Eur J Anesthesiol 1993; 10: 261–6.
  • Aarts L, van der Hee R, Dekker I, de Jong J, Langemeijer H, Bast A. The widely used anesthetic agent propofol can replace alpha-tocopherol as an antioxidant. FEBS Lett 1995; 357: 83–5.
  • Brasil LJ, San-Miguel B, Kreztmann NA, Amaral JL, Zettler CG, Marroni N et al. Halothane induces oxidative stress and NF-κB activation in rat liver: protective effect of propofol. Toxicology 2006; 227: 53–61.
  • Peters CE, Korcok J, Gelb AW, Wilson JX. Anesthetic concentrations of propofol protect against oxidative stress in primary astrocyte cultures: comparison with hypothermia. Anesthesiology 2001; 94: 313–21.
  • Vasileiou I, Xanthos T, Koudouna E, Perrea D, Klonaris C, Katsargyris A et al. Propofol: a review of its non-anesthetic effects. Eur J Pharmacol 2009; 605: 1–8.
  • Shimono H, Goromaru T, Kadota Y, Tsurumaru T, Kanmura Y. Propofol displays no protective effect against hypoxia/ reoxygenation injury in rat liver slices. Anesth Analg 2003; 97: 442–
Turkish Journal of Medical Sciences-Cover
  • ISSN: 1300-0144
  • Yayın Aralığı: 6
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

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