Epidemiology, clinical features, and laboratory detection of Mycoplasma pneumoniae infection in East Azerbaijan, Iran

Mycoplasma pneumoniae is involved in the etiology of many respiratory tract infections. This prospective study describes the epidemiology and clinical properties of M. pneumoniae infections in hospitals of the Tabriz University of Medical Sciences. Materials and methods: From May 2009 to November 2010, 200 patients with respiratory tract infections were enrolled into this study. Due to antibiotic use 51 patients were excluded. M. pneumoniae from respiratory infections was detected by culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assays. Results: M. pneumoniae infection was detected in 9 (6.15%), 8 (5.3%), and 3 (2.01%) of patients by PCR, ELISA, and culture methods, respectively. M. pneumoniae infection was mostly prevalent in patients 21-80 years old. Most infections (92%) were community-acquired and coughs, fever, dyspnea, and malaise were among the most common symptoms. Conclusion: Pneumonia due to the M. pneumoniae infection is infrequent in Tabriz and the clinical symptoms of the patients were determined to be mild. The PCR method also proved to be more sensitive and reliable than ELISA and culture assays in the detection of M. pneumoniae.

Epidemiology, clinical features, and laboratory detection of Mycoplasma pneumoniae infection in East Azerbaijan, Iran

Mycoplasma pneumoniae is involved in the etiology of many respiratory tract infections. This prospective study describes the epidemiology and clinical properties of M. pneumoniae infections in hospitals of the Tabriz University of Medical Sciences. Materials and methods: From May 2009 to November 2010, 200 patients with respiratory tract infections were enrolled into this study. Due to antibiotic use 51 patients were excluded. M. pneumoniae from respiratory infections was detected by culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assays. Results: M. pneumoniae infection was detected in 9 (6.15%), 8 (5.3%), and 3 (2.01%) of patients by PCR, ELISA, and culture methods, respectively. M. pneumoniae infection was mostly prevalent in patients 21-80 years old. Most infections (92%) were community-acquired and coughs, fever, dyspnea, and malaise were among the most common symptoms. Conclusion: Pneumonia due to the M. pneumoniae infection is infrequent in Tabriz and the clinical symptoms of the patients were determined to be mild. The PCR method also proved to be more sensitive and reliable than ELISA and culture assays in the detection of M. pneumoniae.

___

  • Ansarin K, Abedi S, Ghotaslou R, Soroush MH, Ghabili K, Kenneth RC. Infection with Mycoplasma pneumoniae is not related to asthma control, asthma severity, and location of airway obstruction. Int J Gen Med 2011; 4:1–4.
  • Atkinson TP, Balish MF, Waites KB. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol Rev 2008; 32: 956–73.
  • Ma LD, Chen M, Dong Y, Fan J, Xia L, Wang S et al. Rapid Mycoplasma culture for the early diagnosis of Mycoplasma pneumoniae infection. J Clin Lab Anal 2010; 24: 224–9.
  • Foy HM. Infections caused by Mycoplasma pneumoniae and possible carrier state in different populations of patients. Clin Infect Dis 1993; 17: S37–S46.
  • Clyde WA. Clinical overview of typical Mycoplasma pneumoniae infections. Clin Infect Dis 1993; 17: S32–S36.
  • Özakın C, Güler H, Gürcüoğlu E, Bakır Özbey S, Kazak E, Sınırtaş AM. A study on molecular characterization of macrolide resistance mechanism among isolates of Streptococcus pneumoniae from the southern Marmara region of Turkey, as well as resistance to macrolides and penicillin in these isolates. Turk J Med Sci 2012; 42: 137–44.
  • Liu FC, Chen PY, Huang FL, Tasai CR, Lwee CY, Wang LC. Rapid diagnosis of Mycoplasma pneumoniae infection in children by polymerase chain reaction. J Microbiol Immunol Infect 2007; 40: 507–12.
  • Loens K, Ursi D, Goossens H, Iven M. Molecular diagnosis of Mycoplasma pneumoniae respiratory tract infections. J Clin Microbiol 2003; 41: 4915–23.
  • Sasaki T, Kenri T, Okazaki N, Iseki M, Yamashita R, Shintani M et al. Epidemiological study of Mycoplasma pneumoniae infections in Japan based on PCR-restriction fragment length polymorphism of the P1 cytadhesin gene. J Clin Microbiol 1996; 34: 447–9.
  • Waites KB, Talkington DF. Mycoplasma pneumoniae and its role as a human pathogen. Clin Microbiol Rev 2004; 17: 697– 7
  • Dunn JJ, Malan AK, Evans J, Litwin CM. Rapid detection of Mycoplasma pneumoniae IgM antibodies in pediatric patients using ImmunoCard Mycoplasma compared to conventional enzyme immunoassays. Eur J Clin Microbiol Infect Dis 2004; 23: 412–4.
  • Daxboeck F, Krause R, Wenissch C. Laboratory diagnosis of Mycoplasma pneumoniae infection. Clin Microbiol Infect 2003; 9: 263–73.
  • Beersma MF, Dirven K, van Dam AP, Templeton KE, Claas EC, Goossens H. Evaluation of 12 commercial tests and the complement fixation test for Mycoplasma pneumoniae-specific immunoglobulin G (IgG) and IgM antibodies, with PCR used as the “gold standard”. J Clin Microbiol 2005; 43: 2277–85.
  • Al-Rashed A. Role of Mycoplasma pneumoniae in acute respiratory tract infections in Saudi pediatric patients. Ann Trop Med Parasitol 1998; 92: 595–601.
  • Block S. Mycoplasma pneumoniae and Chlamydia pneumoniae in pediatric community-acquired pneumonia: comparative efficacy and safety of clarithromycin versus erythromycin ethylsuccinate. Pediatr Infect Dis J 1995; 14: 471–7.
  • Zer Y, Bayram N, Balcı İ, Filiz A. Investigation of the causative agents for community-acquired pneumonia in adult patients. Turk J Med Sci 2010; 40: 47–52.
  • Naghipour MR, Cuevas LE. Contribution of viruses, Chlamydia spp. and Mycoplasma pneumoniae to acute respiratory infections in Iranian children. J Trop Pediatr 2007; 53: 179–84. Özdemir O, Sarı S, Bakırtaş A, Zorlu P, Ertan Ü. Underlying diseases of recurrent pneumonia in Turkish children. Turk J Med Sci 2010; 40: 25–30.
  • Huong PLT, Thi NT, Nguyet NTT, Van TK, Hang DT, Huong VTT. First report on clinical features of Mycoplasma pneumoniae infections in Vietnamese children. Jpn J Infect Dis 2007; 60: 370–3.
  • Nisar N, Guleria R, Kumar S, Chand Chawla T, Ranjan Biswas N. Mycoplasma pneumoniae and its role in asthma. Postgrad Med J 2007; 83: 100–4.
  • Sağlam L. Investigation of the results of a smoking cessation clinic and the factors associated with success. Turk J Med Sci 2012; 42: 515–22.
Turkish Journal of Medical Sciences-Cover
  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Trends of intestinal parasites prevalence in the Gaza Strip, 1998-2007: the use of government health records

Adnan Ibrahim ALHINDI, Mervat AL-LOUH

Incidental findings on electron beam tomography renal angiography in hypertensive patients

Baki HEKİMOĞLU, Begüm Demirler Şimşir Mustafa Şekip ALTUNKAN

Double-J stent placement with grasping forceps through ureteroscope working channel: a novel technique

Akif KOÇ, Ömer BAYRAK

Sponge in the belly: postoperative imaging findings of oxidised cellulose (Surgicel®)

Gökçe Kaan ATAÇ, Sadi GÜNDOĞDU, Billur DEMİROĞULLARI, İbrahim Onur ÖZEN, Zekiye Safinur KESKİN, Arzu PAMPAL

Trends of intestinal parasites prevalence in the Gaza Strip, 1998–2007: the use of government health records

Adnan Ibrahim ALHINDI, Mervat AL-LOUH

Is there a rise in resistance rates to fosfomycin and other commonly used antibiotics in Escherichia coli-mediated urinary tract infections? A perspective for 2004 – 2011

Alper TÜNGER, Feriha HALL JR ÇİLLİ, Şöhret AYDEMİR, Meltem TAŞBAKAN IŞIKGÖZ, Hüsnü PULLUKÇU, Oğuz Reşat SİPAHİ

Cytokine associated neopterin response of peripheral blood mononuclear cells to in vitro Epstein–Barr virus transformation process of B lymphocytes

Latife Arzu ARAL, Ayşegül YÜCEL ATAK

Assessment of immune responses to hepatitis A vaccination in children aged 1 and 2 years

Solmaz ÇELEBİ, Mustafa Kemal HACIMUSTAFAOĞLU, Yücehan ALBAYRAK, Ayşe Melda SINIRTAŞ

Effects of green tea on ACE gene expression in rat liver in CCl4-induced cirrhosis

Syed Muhammad SHAHID, Rozeena SHAIKH, Sana SHAMIM, Muhammad ISMAIL, Tabassum MAHBOOB

The effect of preemptive thoracic epidural analgesia on long-term wound pain following major thoracotomy

Abdullah CAN, Ali Fuat ERDEM, Yener AYDIN, Ali AHISKALIOĞLU, Hüsnü KÜRŞAD