Epidemiology, clinical features, and laboratory detection of Mycoplasma pneumoniae infection in East Azerbaijan, Iran
Mycoplasma pneumoniae is involved in the etiology of many respiratory tract infections. This prospective study describes the epidemiology and clinical properties of M. pneumoniae infections in hospitals of the Tabriz University of Medical Sciences. Materials and methods: From May 2009 to November 2010, 200 patients with respiratory tract infections were enrolled into this study. Due to antibiotic use 51 patients were excluded. M. pneumoniae from respiratory infections was detected by culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assays. Results: M. pneumoniae infection was detected in 9 (6.15%), 8 (5.3%), and 3 (2.01%) of patients by PCR, ELISA, and culture methods, respectively. M. pneumoniae infection was mostly prevalent in patients 21-80 years old. Most infections (92%) were community-acquired and coughs, fever, dyspnea, and malaise were among the most common symptoms. Conclusion: Pneumonia due to the M. pneumoniae infection is infrequent in Tabriz and the clinical symptoms of the patients were determined to be mild. The PCR method also proved to be more sensitive and reliable than ELISA and culture assays in the detection of M. pneumoniae.
Epidemiology, clinical features, and laboratory detection of Mycoplasma pneumoniae infection in East Azerbaijan, Iran
Mycoplasma pneumoniae is involved in the etiology of many respiratory tract infections. This prospective study describes the epidemiology and clinical properties of M. pneumoniae infections in hospitals of the Tabriz University of Medical Sciences. Materials and methods: From May 2009 to November 2010, 200 patients with respiratory tract infections were enrolled into this study. Due to antibiotic use 51 patients were excluded. M. pneumoniae from respiratory infections was detected by culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assays. Results: M. pneumoniae infection was detected in 9 (6.15%), 8 (5.3%), and 3 (2.01%) of patients by PCR, ELISA, and culture methods, respectively. M. pneumoniae infection was mostly prevalent in patients 21-80 years old. Most infections (92%) were community-acquired and coughs, fever, dyspnea, and malaise were among the most common symptoms. Conclusion: Pneumonia due to the M. pneumoniae infection is infrequent in Tabriz and the clinical symptoms of the patients were determined to be mild. The PCR method also proved to be more sensitive and reliable than ELISA and culture assays in the detection of M. pneumoniae.
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- Ansarin K, Abedi S, Ghotaslou R, Soroush MH, Ghabili K, Kenneth RC. Infection with Mycoplasma pneumoniae is not related to asthma control, asthma severity, and location of airway obstruction. Int J Gen Med 2011; 4:1–4.
- Atkinson TP, Balish MF, Waites KB. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol Rev 2008; 32: 956–73.
- Ma LD, Chen M, Dong Y, Fan J, Xia L, Wang S et al. Rapid Mycoplasma culture for the early diagnosis of Mycoplasma pneumoniae infection. J Clin Lab Anal 2010; 24: 224–9.
- Foy HM. Infections caused by Mycoplasma pneumoniae and possible carrier state in different populations of patients. Clin Infect Dis 1993; 17: S37–S46.
- Clyde WA. Clinical overview of typical Mycoplasma pneumoniae infections. Clin Infect Dis 1993; 17: S32–S36.
- Özakın C, Güler H, Gürcüoğlu E, Bakır Özbey S, Kazak E, Sınırtaş AM. A study on molecular characterization of macrolide resistance mechanism among isolates of Streptococcus pneumoniae from the southern Marmara region of Turkey, as well as resistance to macrolides and penicillin in these isolates. Turk J Med Sci 2012; 42: 137–44.
- Liu FC, Chen PY, Huang FL, Tasai CR, Lwee CY, Wang LC. Rapid diagnosis of Mycoplasma pneumoniae infection in children by polymerase chain reaction. J Microbiol Immunol Infect 2007; 40: 507–12.
- Loens K, Ursi D, Goossens H, Iven M. Molecular diagnosis of Mycoplasma pneumoniae respiratory tract infections. J Clin Microbiol 2003; 41: 4915–23.
- Sasaki T, Kenri T, Okazaki N, Iseki M, Yamashita R, Shintani M et al. Epidemiological study of Mycoplasma pneumoniae infections in Japan based on PCR-restriction fragment length polymorphism of the P1 cytadhesin gene. J Clin Microbiol 1996; 34: 447–9.
- Waites KB, Talkington DF. Mycoplasma pneumoniae and its role as a human pathogen. Clin Microbiol Rev 2004; 17: 697– 7
- Dunn JJ, Malan AK, Evans J, Litwin CM. Rapid detection of Mycoplasma pneumoniae IgM antibodies in pediatric patients using ImmunoCard Mycoplasma compared to conventional enzyme immunoassays. Eur J Clin Microbiol Infect Dis 2004; 23: 412–4.
- Daxboeck F, Krause R, Wenissch C. Laboratory diagnosis of Mycoplasma pneumoniae infection. Clin Microbiol Infect 2003; 9: 263–73.
- Beersma MF, Dirven K, van Dam AP, Templeton KE, Claas EC, Goossens H. Evaluation of 12 commercial tests and the complement fixation test for Mycoplasma pneumoniae-specific immunoglobulin G (IgG) and IgM antibodies, with PCR used as the “gold standard”. J Clin Microbiol 2005; 43: 2277–85.
- Al-Rashed A. Role of Mycoplasma pneumoniae in acute respiratory tract infections in Saudi pediatric patients. Ann Trop Med Parasitol 1998; 92: 595–601.
- Block S. Mycoplasma pneumoniae and Chlamydia pneumoniae in pediatric community-acquired pneumonia: comparative efficacy and safety of clarithromycin versus erythromycin ethylsuccinate. Pediatr Infect Dis J 1995; 14: 471–7.
- Zer Y, Bayram N, Balcı İ, Filiz A. Investigation of the causative agents for community-acquired pneumonia in adult patients. Turk J Med Sci 2010; 40: 47–52.
- Naghipour MR, Cuevas LE. Contribution of viruses, Chlamydia spp. and Mycoplasma pneumoniae to acute respiratory infections in Iranian children. J Trop Pediatr 2007; 53: 179–84. Özdemir O, Sarı S, Bakırtaş A, Zorlu P, Ertan Ü. Underlying diseases of recurrent pneumonia in Turkish children. Turk J Med Sci 2010; 40: 25–30.
- Huong PLT, Thi NT, Nguyet NTT, Van TK, Hang DT, Huong VTT. First report on clinical features of Mycoplasma pneumoniae infections in Vietnamese children. Jpn J Infect Dis 2007; 60: 370–3.
- Nisar N, Guleria R, Kumar S, Chand Chawla T, Ranjan Biswas N. Mycoplasma pneumoniae and its role in asthma. Postgrad Med J 2007; 83: 100–4.
- Sağlam L. Investigation of the results of a smoking cessation clinic and the factors associated with success. Turk J Med Sci 2012; 42: 515–22.