The effects of adding methylprednisolone to low-potassium dextran solution on preserved ischemic lungs
To investigate the effects of adding methylprednisolone to low-potassium dextran solution on cold-preserved ischemic lungs. Materials and methods: The study included 42 rat lung blocks that were divided into 7 groups (n = 6). Lung blocks were preserved in 40 mL of cold Perfadex solution, to which 5, 15, 30, 60, and 120 mg kg-1 (approximately 40, 120, 240, 480, and 960 mg L-1) of methylprednisolone were added. After a 24-h preservation period, the lungs were removed from the solution and examined. Results: As compared to the Perfadex only group, cell death and degeneration were significantly higher in the Perfadex + methylprednisolone groups (P < 0.05). MDA and IL-8 levels were higher in all Perfadex + methylprednisolone groups. Statistically significant differences were observed between some of these groups. Antioxidant enzyme activity was generally higher in the Perfadex + methylprednisolone groups than in the Perfadex only group (P < 0.05). Conclusion: Adding methylprednisolone to low-potassium dextran solution does not have any beneficial effects but has possible deleterious effects in rat lung preservation.
The effects of adding methylprednisolone to low-potassium dextran solution on preserved ischemic lungs
To investigate the effects of adding methylprednisolone to low-potassium dextran solution on cold-preserved ischemic lungs. Materials and methods: The study included 42 rat lung blocks that were divided into 7 groups (n = 6). Lung blocks were preserved in 40 mL of cold Perfadex solution, to which 5, 15, 30, 60, and 120 mg kg-1 (approximately 40, 120, 240, 480, and 960 mg L-1) of methylprednisolone were added. After a 24-h preservation period, the lungs were removed from the solution and examined. Results: As compared to the Perfadex only group, cell death and degeneration were significantly higher in the Perfadex + methylprednisolone groups (P < 0.05). MDA and IL-8 levels were higher in all Perfadex + methylprednisolone groups. Statistically significant differences were observed between some of these groups. Antioxidant enzyme activity was generally higher in the Perfadex + methylprednisolone groups than in the Perfadex only group (P < 0.05). Conclusion: Adding methylprednisolone to low-potassium dextran solution does not have any beneficial effects but has possible deleterious effects in rat lung preservation.
___
- Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterisation of erythrocyte glutathione peroxidase. J Lab & Clin Med 1967; 70: 158-169.
- Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hyroxynonenal. Methods Enzymol 1990; 186: 407-21.
- Aeba R, Killinger WA, Keenan RJ, Yousem SA, Hamamoto I, Hardesty RL et al. Lazaroid U74500A as an additive to University of Wisconsin solution for pulmonary grafts in the rat transplant model. J Thorac Cardiovasc Surg 1992; 104: 1333-9.
- Kuwaki K, Komatsu K, Sohma H, Abe T. The effect of various doses of lazaroid U74389G on lung ischemia reperfusion injury. Thorac Cardiovasc Surg 1999; 47: 67-72.
- Killinger WA Jr, Dorofi DB, Keagy BA, Johnson G Jr. Improvement of endothelial cell viability at 4 degrees C by addition of lazaroid U74500A to preservation solutions. Transplantation 1992; 53: 983-6.
- Hisatomi K, Isomura T, Sato T, Hayashida N, Kosuga K, Ohishi K. Beneficial effect of steroid on myocardial preservation in isolated rat hearts Jpn Circ J 1995; 59: 815-23.
- Hall TS, Borkon AM, Gurtner GC, Brawn J, Hutchins GM, Reitz BA et al. Improved static lung preservation with corticosteroids and hypothermia. J Heart Transplant 1988; 7: 348-52.
- Hiramatsu Y, Muraoka R, Chiba Y, Sasaki M. Influence of pH of preservation solution on lung viability. Ann Thorac Surg 1994; 58: 1083-6.
- Martin DR, Scott DF, Downes GL, Belzer FO. Primary cause of unsuccessful liver and heart preservation: cold sensitivity of the ATPase system. Ann Surg 1972; 175: 111-7.
- Schramm CM, Grunstein MM. Corticosteroid modulation of Na(+)-K+ pump-mediated relaxation in maturing airway smooth muscle. Br J Pharmacol. 1996; 119: 807-12.
- Fischer S, Maclean AA, Liu M, Cardella JA, Slutsky AS, Suga M et al. Dynamic changes in apoptotic and necrotic cell death correlate with severity of ischemia-reperfusion injury in lung transplantation. Am J Respir Crit Care Med 2000; 162: 1932-9.
- Fisher AJ, Donnelly SC, Hirani N, Haslett C, Strieter RM, Dark JH et al. Elevated levels of interleukin-8 in donor lungs is associated with early graft failure after lung transplantation. Am J Respir Crit Care Med 2001; 163: 259-65.
- Kaneda H, Waddell TK, de Perrot M, Bai XH, Gutierrez C, Arenovich T et al. Pre-implantation multiple cytokine mRNA expression analysis of donor lung grafts predicts survival after lung transplantation in humans. Am J Transplant 2006; 6: 544- 51.
- Arbak S, Yalin A, Ercan F. Effects of preservation of rat lungs in a hypothermic medium on alveolar morphology. Acta Histochem 1999; 101: 341-9.
- Yeginsu A, Ergin M. Antioxidant enzymes and lipid peroxidation in cold ischemic lung preservation experimental and clinical transplantation. 2009; 2: 94-8.
- Pryor WA, Houk KN, Foote CS, Fukuto JM, Ignarro LJ, Squadrito GL et al. Free radical biology and medicine: it’s a gas, man Am J Physiol Regul Integr Comp Physiol 2006; 291: R491- R511.
- Yang W, Block ER. Effect of hypoxia and reoxygenation on the formation and release of reactive oxygen species by porcine pulmonary artery endothelial cells. J Cell Physiol 1995; 164: 414-423.
- Quinlan T, Spivack S, Mossman BT. Regulation of antioxidant enzymes in lung after oxidant injury. Environ Health Perspect 1994; 102 (suppl 2): 79-87.
- Heffner JE, Repine JE. Pulmonary strategies of antioxidant defense. Am Rev Respir Dis 1989; 140: 531-554.