Sahra KIRMUSAOĞLU, Seyhun YURDUGÜL, M. Esra KOÇOĞLU

The effect of N-acetylcysteine on growth and biofi lm formation in Staphylococcus epidermidis strains

Amaç: Bu çalışmanın amacı N-asetilsisteinin (NAC) hastaneye yatmış ve bağışıklık sistemi baskılanmış hastalarda, tüm dünyada implant ile ilişkili infeksiyonlar gibi çok önemli sorunlara neden olan Staphylococcus epidermidis suşlarında biyofilm oluşumundaki olası önleyici etkilerini incelemekti. Yöntem ve gereç: NACnin çeşitli konsantrasyonlarının 28 Staphylococcus epidermidis suşu tarafından oluşturulan biyofilm üzerine etkisi mikroelisa yöntemiyle spektrofotometrik olarak test edildi. Bulgular: NACnin çeşitli konsantrasyonlarının varlığında 15 metisilin dirençli Staphylococcus epidermidis ve 13 metisilin duyarlı Staphylococcus epidermidis suşlarında biyofilm oluşturma üzerinde anlamlı farklılıklar bulundu (P < 0,05). Sonuç: NACnin artan konsantrasyonlarından dolayı yapışkan tabaka oluşumunda azalma görüldü. Bu bulgulara göre, kolonizasyonu ve yabancı cisim infeksiyonlarını önlemek için NAC in medikal aletlerde ya da kateter solüsyonlarında antibiyofi lm ajan olarak kullanımı mümkündür.

N-asetilsisteinin Staphylococcus epidermidis suşlarında üreme ve biyofilm oluşturma üzerine etkisi

Aim: To examine the possible preventive effect of N-acetylcysteine (NAC) on biofilm formation in Staphylococcus epidermidis strains, which cause very important problems such as implant-associated infections in hospitalized and immunocompromised patients worldwide. Materials and methods: The effects of varying concentrations of NAC on the formation of biofilm by 28 strains of Staphylococcus epidermidis was investigated spectrophotometrically by micro-ELISA assay. Results: Significant differences in biofi lm formation in the presence of various NAC concentrations were found in 15 methicillin-resistant Staphylococcus epidermidis and 13 methicillin-sensitive Staphylococcus epidermidis strains (P < 0.05). Conclusion: It was found that slime formation was decreased due to the increased concentrations of the NAC treatments. Based on these findings, it is possible to use NAC as an antibiofi lm agent on medical devices or in catheter lock solutions to prevent colonization and implant-associated infections.

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