Hayati Murat AKGÜL, Mustafa GÖREGEN, Cemal GÜNDOĞDU

Th e cytomorphological analysis of buccal mucosa cells in smokers

Amaç: Araştırmanın amacı sigara kullanımının normal yanak mukozasına etkisinin sitomorfolojik olarak incelenmesidir. Yöntem ve gereç: Çalışmaya yaşları 40-60 arasında değişen, sigara kullanan 23 (14 erkek, 9 kadın) ve kullanmayan 17 (10 erkek, 7 kadın) olmak üzere toplam 40 birey dahil edildi. Bireylerden fırça ile yanak epitel döküntü hücreleri alındı ve özel bir bilgisayar programı kullanılarak hücrelerin sitomorfometrik ölçümleri yapıldı. Bulgular: Hücre nükleuslarına ait ortalama nükleer alan (NA), nükleer çevre (NÇ), minimal çap (Çapmin) ve maksimal çap (Çapmax) değerleri Kontrol grubunda sırasıyla, 46,00 ± 11,31, 28,18 ± 2,43, 6,18 ± 0,88 and 10,00 ± 0,94 olarak bulunurken; Çalışma grubunda sırasıyla 53,61 ± 7.29, 29,52 ± 2,02, 6,87 ± 0,63 and 10,52 ± 0,67 olarak bulundu. Her iki gruptaki bu parametrelerden NA, Çapmin ve Çapmax arasında istatistiksel olarak anlamlı farklılık bulunurken, (t = 2.586, P = 0.014; t = 2.909, P = 0,006; t = 2.064, P = 0,046, sırasıyla), NÇ değerleri arasında istatistiksel olarak anlamlı farklılık bulunmadı (P > 0,05). Sonuç: Nükleer alanda belirlenen artış, sigaraya bağlı hücresel adaptasyonu göstermektedir. Hücre çekirdeğindeki adaptif değişimi, displastik değişikliğe yönelim olarak yorumlamak mümkündür.

Sigara içen bireylerde normal yanak mukozasinin kantitatif sitolojik olarak incelenmesi

Aim: To examine the eff ect of smoking on normal buccal mucosa cytomorphologically. Materials and methods: Forty individuals aged between 40 and 60, comprising 23 smokers (14 male, 9 female) and 17 and non-smokers (10 male, 7 female), were included in the study. Th e buccal epithelial cells of these individuals were collected with a brush and the cells were measured cytomorphometrically using soft ware. Results: Th e average nuclear area (NA), nuclear perimeter (NP), minimal nuclear diameter (D-min), and maximal nuclear diameter (D-max) were 46.00 ± 11.31, 28.18 ± 2.43, 6.18 ± 0.88, and 10.00 ± 0.94, respectively, in the control group, and 53.61 ± 7.29, 29.52 ± 2.02, 6.87 ± 0.63 and 10.52 ± 0.67 in the study group, respectively. While there was a statistically signifi cant diff erence in NA, D-min, and D-max (t = 2.586, P = 0.014; t = 2.909, P = 0.006; t = 2.064, P = 0.046, respectively), there was no statistically signifi cant diff erence in NP between the 2 groups (P > 0.05). Conclusion: Th is increase determined in NA shows smoking-related cellular adaptation. It is possible to conclude that this adaptive change in the cell nucleus tends to be a dysplastic change.

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