Hüseyin Avni UYDU, Arife Pınar EKİNCİ, Mehtap ATAK, Adem DEMİR

Protective roles of Cimin grape tissues on oxidative stress markers in the cellular system model

To analyze the phenolic composition of the Cimin grape, which is 1 of 2 grape varieties with a protected geographical indication in Turkey and is used locally to treat the symptoms of some disorders such as impotence and cardiovascular diseases, and to investigate its antioxidant potency against oxidant mediators in the models of intra- and extracellular surroundings. Materials and methods: Cimin grape samples were classified into 5 groups according to the grape's tissues and the extraction solvents used. Free radical scavenging (DPPH) and antilipid peroxidation product [thiobarbituric acid reactive substance (TBARS) and conjugated diene (CD)] levels of the grape tissues were extrapolated from the measurement of total phenolic and individual monomeric flavanol contents in each extract. Results: The seed methanolic extract had the highest total amount of flavanols, with the amount of catechin at 4.034 mM. The DPPH activities of the seed extracts were 2- to 10-fold higher than those of the other samples. The seed extract-treated erythrocyte and unfractionated human plasma also showed lower TBARS and CD values. In addition, regeneration of glutathione was more obvious in grape seed extracts than in the rest of the tissues. Conclusion: The underlying mechanism of these changes can be related mainly to increased antioxidant status. Cimin grape consumption may have beneficial effects on health maintenance.

Anahtar Kelimeler:

Key words: Cimin grape, flavanols, glutathione, reactive oxygen species, thiobarbituric acid reactive substance

Protective roles of Cimin grape tissues on oxidative stress markers in the cellular system model

Keywords:

Key words: Cimin grape, flavanols, glutathione, reactive oxygen species, thiobarbituric acid reactive substance,

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Waris G, Ahsan H. Reactive oxygen species: role in the development of cancer various chronic conditions. J Carcinog 2006; 11: 5–14.
Ağar G, Aslan A, Sarıoğlu Kotan E, Alpsoy L, Çeker S. Protective activity of the methanol extract of Usnea longissima against oxidative damage and genotoxicity caused by afl aflatoxin B1 in vitro. Turk J Med Sci 2011; 41: 1043–1049.
Ajila, CM, Parasada Rao UJS. Protection against hydrogen peroxide induced oxidative damage in rat erythrocytes by Mangifera indica L. peel extract. Food Chem Toxicol 2008; 46: 303–309.
Sayın O, Arslan N, Güner G. Resveratrol cardiovascular system. Turk J Biochem 2008; 33: 117–121.
Kaviarasan S, Naik GH, Gangabhagirathi R, Anuradha CV, Priyadarsini KI. In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds. Food Chem 2007; 103: 31–37.
Tedesco I, Russo GL, Nazzaro F, Russo M, P. Antioxidant effect of red wine anthocyanins in normal and catalase-inactive human erythrocytes. J Nutr Biochem 2001; 12: 505–511.
O’Byrne DJ, Devaraj S, Grundy SM, Jialal I. Comparison of the antioxidant effects of Concord grape juice flavonoids alphatocopherol on markers of oxidative stress in healthy adults. Am J Clin Nutr 2002; 76: 1367–1374.
Aydın M, Çelik S. Effects of lycopene on plasma glucose, insulin levels oxidative stress, and body weights of streptozotocininduced diabetic rats. Turk J Med Sci 2012; 42: 1406–1413.
Schnitzer E, Pinchuk I, Fainaru M, Schafer Z, Lichtenberg D. Copper-induced lipid oxidation in unfractionated plasma: the lag preceding oxidation as a measure of oxidation-resistance. Biochem Bioph Res Co 1195; 216: 854–861.
Bozan B, Tosun G. Study of polyphenol content in the seeds of red grape (Vitis vinifera L.) varieties cultivated in Turkey their antiradical activity. Food Chem 2008; 109: 426–430.
Üzümlü District Governorate. Cimin Üzümü. Erzincan, Turkey; 2010 (in Turkish; available online at http://www.uzumlu.gov.tr/ index.php/yoeresel/175-cimin-uezuemue).
Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. Am J Enol Viticult 1977; 28: 49–
International Organization for Standardization. Determination of substances characteristic of green and black tea. ISO 14502-2. Geneva, Switzerland: ISO; 2005.
Cuendet M, Hostettmann P, Potterat O. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helv Chim Acta 1997; 80: 1144–1152.
Drabkin DL, Austin JM. Spectrophotometric constants for common hemoglobin derivatives in human, dog and rabbit blood. J Biol Chem 1932; 98: 719–733.
Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882–888.
Stocks J, Dormy TL. The autoxidation of human red cell lipids induced by hydrogen peroxide. Brit J Haematol 1971; 20: 95–111.
Durak I, Elgün S, Bingöl K, Burak M, Cimen Y, Kaçmaz M, Büyükkoçak S, Öztürk S. Effects of cigarette smoking with different tar content on erythrocyte oxidant/antioxidant status. Addict Biol 2002; 7: 255–258.
Wills EO. Evaluation of lipid peroxidation in lipids and biological membranes. In: Snell K, Mullock B, editors. Biochemical Toxicology. A Practical Approach. Oxford, UK: IRL Press; 1987. pp. 127–151.
Spranger T, Finckh B, Fingerhut R, Kohlschütter A, Beisiegel U, Kontush A. How different constituents of human plasma low density lipoprotein determine plasma oxidizability by copper. Chem Phys Lipids 1998; 98: 39–52.
Konyalioglu S, Karamenderes C. The protective effects of Achillea L. species native in Turkey against H 2 O 2 -induced oxidative damage in human erythrocytes and leucocytes. J Ethnopharmacol 2005; 102: 221–227.
Rabai M, Toth A, Kenyeres P, Mark L, Marton Z, Juricskay I, Toth K, Czopf L. In vitro hemorheological effects of red wine and alcohol-free red wine extract. Clin Hemorheol Micro 2010; 44: 227–236.
Downey MO, Mazza M, Krstic MP. Development of a stable extract for anthocyanins and flavonols from grape skin. Am J Clin Nutr 2007; 58: 358–364.
Lacopini P, Baldi M, Storchi P, Sebastiani L. Catechin epicatechin, quercetin, rutin and resveratrol in red grape: content, in vitro antioxidant activity interactions. J Food Compos Anal 2008; 21: 589–598.
Carini M, Aldini G, Bombrelli E, Morazon P. UVB-induced hemolysis of rat erythrocytes: protective effect of procyanidins from grape seeds. Life Sci 2000; 67: 1799–1814.
Froehlicher T, Hennebelle T, Martin-Nizard F, Cleenewerk P, Hilbert JL, Trotin F, Grec S. Phenolic profiles and antioxidative effects of hawthorn cell suspensions, fresh fruits, medicinal dried parts. Food Chem 2009; 115: 897–903.
Bakkalbasi E, Yemis O, Aslanova D. Major ﬂavan-3-ol composition and antioxidant activity of seeds from different from varieties grown in Turkey. Eur Food Res Technol 2005; 221: 792–797.
Avcı A, Atlı T, Ergüder İ, Varlı M, Devrim E, Demir Ö, Durak İ, Turgay M. Effects of grape consumption on plasma and erythrocyte antioxidant parameters in elderly subjects. Turk J Med Sci 2010; 40: 525–529.
Sadeghipour M, Terreux R, Phipps J. Flavonoids and tyrosine nitration: structure-activity relationship correlation with enthalpy of formation. Toxicol In Vitro 2005; 19: 155–165.
Yen FL, Wu TH, Lin LT, Cham TM, Lin CC. Concordance between antioxidant activities and flavanol contents in different extracts fractions of Cuscuta chinensis. Food Chem 2008; 108: 445–462.
Rodriguez J, Di Pierro D, Gioia M, Monaco S, Delgado R, Coletta M, Marini S. Effects of natural extract from Mangifera indica L. and its active compound, mangiferin, on energy state lipid peroxidation of red blood cells. Biochim Biophys Acta 2006; 1760: 1333–1342.
Kiruthiga PV, Shafreen RB, Pian SK, Arun S, Govindu S, Devi KP. Protective effect of silymarin on erythrocyte haemolysate against benzo(α)pyrene and exogenous reactive oxygen species (H 2 O 2 ) induced oxidative stress. Chemosphere 2007; 68: 1511– 15
Lam RY, Woo AY, Leung PS, Cheng CH. Antioxidant actions of phenolic compounds found in dietary plants on low-density lipoprotein and erythrocytes in vitro. J Am Coll Nutr 2007; 26: 233–242.
Ko CH, Li K, Ng PC, Fung LP, Li CL, Wong RP, Chui KM, Gu GJ, Yung E, Wang CC et al. Pro-oxidative effects of tea polyphenols, epigallocatechin-3-gallate epigallocatechin, on G6PD-deficient erythrocytes in vitro. Int J Mol Med 2006; 18: 987–994.
Ault JG, Lawrence DA. Glutathione distribution in normal and oxidatively stressed cells. Exp Cell Res 2003; 285: 9–14.
Isla MI, Nieva Moreno MI, Sampietro AR, Vattuone MA. Antioxidant activity of Argentine propolis extracts. J Ethnopharmacol 2001; 76: 165–170.
Dohadwala MM. Grapes and cardiovascular disease. J Nutr 2009; 139: 1788–1793.
Young JT, Dragsted LO, Daneshvar B, Lauridsen ST, Hansen M, Sandström B. The effect of grape-skin extract on oxidative status. Brit J Nutr 2004; 84: 505–513.