Partial Purification of Topoisomerase I from Mycobacterium phlei

Aim: DNA topoisomerases control several cellular activities by catalyzing the relaxation of superhelices, which are produced during the replication of DNA. Investigation of the inhibitory action of the candidate substances on topoisomerase activity is widely used in drug development. The purpose of this study was to purify topoisomerase I from Mycobacterium phlei, which is closely related to Mycobacterium tuberculosis, and investigate some of its properties. Materials and Methods: Topoisomerase I from Mycobacterium phlei was partially purified with the method including homogenization followed by DNase treatment, Sephadex G50 and Heparin Sepharose column chromatography steps. Results: The enzyme was purified 31.8-fold with a yield of 16.7%. Molecular weight of the enzyme was estimated to be 125 kDa. Enzyme activity was found to be stable in the pH range of 6.0 - 8.5. ATP and spermidine were not required for the activity of the enzyme. Camptothecin, which is an inhibitor of eukaryotic topoisomerase I, did not inhibit the enzyme at the concentrations studied. Conclusions: The investigated properties of partially purified topoisomerase I from nonpathogenic strain Mycobacterium phlei were found closely related to those of Mycobacterium tuberculosis. Characterization of the enzyme that is completely purified with additional purification steps will hopefully lead to the development of selective antimycobacterial drugs.

Anahtar Kelimeler:

Mycobacterium phlei, DNA topoisomerase type I, purification, molecular properties

Partial Purification of Topoisomerase I from Mycobacterium phlei

Keywords:

Mycobacterium phlei, DNA topoisomerase type I, purification, molecular properties,

PDF

___

Kung VT, Wang JC. Purification and characterization of an omega protein from Micrococcus luteus. J Biol Chem 1977; 252: 5398- 402.
Störl K, Störl HJ. DNA topoisomerase I from Diplococcus pneumoniae. Biomed Biochim Acta 1989; 48: 69-76.
Slesarev, AI, Zaitzev DA, Kopylov VM, Stetter KO, Kozyavkin SA. DNA topoisomerase III from extremely thermophilic archeabacteria. ATP-independent type I topoisomerase from Desulfurococcus amylolyticus drives extensive unwinding of closed circular DNA at high temperature. J Biol Chem 1991; 266: 12321-8.
Bouthier de la Tour C, Portemer C, Forterre P, Huber R, Duguet M. ATP-independent DNA topoisomerase from Fervidobacterium islandicum. Biochim Biophys Acta 1993; 1216: 213-20.
Alkorta I, Park C, Kong J, Garbisu C, Albeti M, Pon N et al. Rhodobacter capsulatus DNA topoisomerase I purification and characterization. Arch Biochem Biophys 1999; 362: 123-30.
Bhaduri T, Nagaraja V. DNA topoisomerase I from Mycobacterium smegmatis. Indian J Biochem Biophys 1994; 31: 339-43.
Bhaduri T, Bagui TK, Sikder D, Nagaraja V. DNA topoisomerase I from Mycobacterium smegmatis. An enzyme with distinct features. J Biol Chem 1998; 273: 13925-32.
Yang F, Lu G, Rubin H. Cloning, expression, purification and characterization of DNA topoisomerase I of Mycobacterium tuberculosis. Gene 1996; 178: 63-9.
World Health Organization. 2006 Global Report. Available from: URL: http://www.who.int/tb/publications/global_report/2006/ pdf/full_report_correctedversion.pdf
Durmaz R, Zozio T, Gunal S, Allix C, Fauville-Dufaux M, Rastogi N. Population-based molecular epidemiological study of tuberculosis in Malatya, Turkey. J Clin Microbiol 2007; 45: 4027- 35.
Goldman RC, Plumley KV, Laughon BE. The evolution of extensively drug resistant tuberculosis (XDR-TB): history, status and issues for global control. Infect Disord Drug Targets 2007; 7: 73-91.
Sambrook J, Russell DW. Preparation of plasmid DNA by alkaline lysis with SDS: maxipreparation. In: Molecular Cloning: A Laboratory Manual, 3rd ed. New York: Cold Spring Harbor Laboratory Press; 2001. pp.1.38-1.41.
Stewart L, Champoux JJ. Assaying DNA topoisomerase I relaxation activity. Methods Mol Biol 2001; 95: 1-11.
Gellert M, Mizuuchi K, O’Dea MH, Nash HA. DNA gyrase: an enzyme that introduces superhelical turns into DNA. Proc Natl Acad Sci USA 1976; 73: 3872-6.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5.
Nadal M, Jaxel C, Portemer C, Forterre P, Mirambeau G, Duguet M. Reverse gyrase of Sulfolobus: purification to homogeneity and characterization. Biochemistry 1988; 27: 9102-8.
Bouthier de la Tour C, Portemer C, Kaltoum H, Duguet M. Reverse gyrase from the hyperthermophilic bacterium Thermotoga maritima: properties and gene structure. J Bacteriol 1998; 180: 274-81.
Anderluzzi D, Pedrini AM. Structural similarities between M. luteus and E. coli DNA topoisomerase I. Biochem Biophys Res Commun 1993; 192: 657-64.
Srivenugopal KS, Morris DR. Differential modulation by spermidine of reactions catalyzed by type 1 prokaryotic and eukaryotic topoisomerases. Biochemistry 1985; 24: 4766-71.