Malondialdehyde and nitric oxide levels and catalase, superoxide dismutase, and glutathione peroxidase levels in maternal blood during diferent trimesters of pregnancy and in the cord blood of newborns

Malondialdehyde and nitric oxide levels and catalase, superoxide dismutase, and glutathione peroxidase levels in maternal blood during diferent trimesters of pregnancy and in the cord blood of newborns

Background/aim: To determine whether levels of malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathioneperoxidase (GPx), and catalase (CAT) change during the 3 trimesters of pregnancy, and to compare the third trimester of pregnancywith newborn cord blood in respect to the levels of MDA, NO, and antioxidant enzymes.Materials and methods: Maternal blood samples were collected at 9 13, 22 26, and 36 40 weeks of gestation. Cord blood was collectedat the time of delivery.Results: NO levels and GPx activity were higher in the second and third trimesters than in the frst trimester. MDA level was lower in thethird trimester and SOD and CAT activities were lower in the second and third trimesters than the frst trimester. In cord blood, MDAand NO levels were lower while CAT and GPx activities were higher than in the third trimester of pregnancy.Conclusion: Tese results suggest that the balance of free radical and antioxidant production during pregnancy ensures healthy fetusgrowth and development.

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  • 1. Agarwal A, Gupta S, Sekhon L, Shah R. Redox considerations in female reproductive function and assisted reproduction: from molecular mechanisms to health implications. Antioxid Redox Sign 2008; 10: 1375–1403.
  • 2. Csapo AI, Pulkkinen MO, Ruttner B, Sauvage JP, Wiest WG. Te signifcance of the human corpus luteum in pregnancy maintenance. I. Preliminary studies. Am J Obstet Gynecol 1972; 112: 1061–1067.
  • 3. Myatt L. Placental adaptive responses and fetal programming. J Physiol 2006; 572: 25–30.
  • 4. Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: reference interval and efects of life style factors. Clin Chem 1997; 43: 1209–1214.
  • 5. Little RE, Gladen BC. Levels of lipid peroxides in uncomplicated pregnancy: a review of the literature. Reprod Toxicol 1999; 13: 347–352.
  • 6. Qanungo S, Sen A, Mukherjea M. Antioxidant status and lipid peroxidation in human feto-placental unit. Clin Chim Acta 1999; 285: 1–12.
  • 7. Takehara Y, Yoshioka T, Sasaki J. Changes in the levels of lipoperoxide and antioxidant factors in human placenta during gestation. Acta Med Okayama 1990; 44: 103–111.
  • 8. Arıkan S, Konukoğlu D, Arıkan Ç, Akçay T, Davas I. Lipid peroxidation and antioxidant status in maternal and cord blood. Gynecol Obstet Inves 2001; 51: 145–149.
  • 9. Pentieva K, Ivanova L, Petrova S, Ovcharova D, Vatralova K, Angelova K. Changes in the level of lipid peroxidation in healthy pregnant women. Akush Ginekol (Sofia) 1995; 34: 19–21 (in Bulgarian with abstract in English).
  • 10. Buonocore G, Perrone S. Biomarkers of oxidative stress in the fetus and newborn. Hematology 2006; 2: 103–107.
  • 11. Nakai A, Oya A, Kobe H, Asakura H, Yokota A, Koshino T, Araki T. Changes in maternal lipid peroxidation levels and antioxidant enzymatic activities before and afer delivery. J Nippon Med Sch 2000; 67: 434–439.
  • 12. Upadhyaya C, Mishra S, Singh PP, Sharma P. Antioxidant status and peroxidative stress in mother and newborn - a pilot study. Indian J Clin Biochem 2005; 20: 30–34.
  • 13. Lurie S, Matas Z, Boaz M, Fux A, Golan A, Sadan O. Diferent degrees of fetal oxidative stress in elective and emergent cesarean section. Neonatology 2007; 92: 111–115.
  • 14. Jo T, Takauchi Y, Nakajima Y, Fukami K, Kosaka H, Terade N. Maternal or umbilical venous levels of nitrite/nitrate during pregnancy and at delivery. In Vivo 1998; 12: 523–526.
  • 15. Kart A, Çelik Ç, Tuncer S, Acar A, Pirbudak L, Çapar M. Farklı doğum tiplerinde anne ve yeni doğan bebeklerinde oksidan stres. Turkiye Klinikleri J Gynecol Obst 2001; 11: 136–141 (in Turkish).
  • 16. Kumar A, Ranjan R, Basu S, Khanna HD, Bhargava V. Antioxidant levels in cord blood of low birth weight newborns. Indian J Pediatr 2008; 45: 583–585.
  • 17. Kurutaş E, Kılınç M, Güler F, Kıran G. Kordon kanında hematolojik parametreler ve antioksidan enzim düzeyleri. Çukurova Med J 2003; 28: 69–73 (in Turkish).
  • 18. Baydaş G, Karataş F, Gursu M, Bozkurt H, Ilhan N, Yaşar A, Canatan H. Antioxidant vitamin levels in term and preterm infants and their relation to maternal vitamin status. Arch Med Res 2002; 33: 276–280.
  • 19. Cengiz C, Kimya Y. Maternal fzyoloji. In: Kişnişçi HA, Gökşin E, Durukan T, Üstay K, Ayhan A, Gürgan T, editors. Temel Kadın Hastalıkları ve Doğum Bilgisi. Ankara, Turkey: Güneş Kitabevi; 1996. pp. 239–243.
  • 20. Saker M, Soulimane Mokhtari N, Merzouk SA, Merzouk H, Belarbi B, Narce M. Oxidant and antioxidant status in mothers and their newborns according to birthweight. Eur J Obstet Gyn R B 2008; 141: 95–99.
  • 21. Toescu V, Nuttall SL, Martin U, Kendall MJ, Dunne F. Oxidative stress and normal pregnancy. Clin Endocrinol 2002; 57: 609– 613.
  • 22. Stipek S, Mechurova A, Crkovska J, Zima T, Platenik J. Lipid peroxidation and superoxide dismutase activity in umbilical and maternal blood. Biochem Mol Biol Int 1995; 35: 705–711.
  • 23. Uotila J, Tuimala R, Aarnio T, Pyykko K, Ahoputa M. Lipid peroxidation product, selenium dependent glutathione peroxidase and vitamin E in normal pregnancy. Eur J Obstet Gyn R B 1991; 42: 95–100.
  • 24. Saikumar P, Jaya B, Renuka Devi MR. Oxidative stress in pregnancy. IOSR Journal of Dental and Medical Sciences 2013; 3: 12–13.
  • 25. Kawashiro Y, Ishii K, Hosoyamada Y, Miyaso H, Matsuno Y, Kubonoya K, Mori C, Hanazato M. Changes in diacron-reactive oxygen metabolites and biological antioxidant potential in maternal serum during pregnancy. FASEB J 2014; 28: 910.6.
  • 26. Walsh SW, Wang Y, Jesse R. Peroxide induces vasoconstriction in the human placenta by stimulating thromboxane. Am J Obstet Gynecol 1993; 169: 1007–1012.
  • 27. Mihailovic M, Cvetkovic M, Ljubic A. Selenium and malondialdehyde content and glutathione peroxidase activity in maternal and umbilical cord blood and amniotic fuid. Biol Trace Elem Res 2000; 73: 47–54.
  • 28. Karabulut AB, Öztürk İÇ, Sezgin N, Hasçalık Ş, Kafaslı H. Preeklamptik gebe kadınlarda ve bebeklerinin kordon kanında, nitrik oksit metabolitleri olan nitrit ve nitratın plazma düzeylerinin araştırılması. J Turgut Özal Med Cent 2001; 8: 1–4 (in Turkish).
  • 29. Hata T, Hashiomoto M, Kanenishi K, Akiyama M, Yanagihara T, Masumura S. Maternal circulation nitrite levels are decreased in both normal normotensive pregnancies and pregnancies with preeclampsia. Gynecol Obstet Inves 1999; 48: 93–97.
  • 30. Brown MA, Tibben E, Zammit VC, Cario GM, Carlton MA. Nitric oxide excretion in normal and hypertensive pregnancies. Hypertens Pregnancy 1995; 14: 319–326.
  • 31. Smarason AK, Alman KG, Young D, Redman CWG. Elevated levels of serum nitrate, a stable end product of nitric oxide, in women with preeclampsia. Br J Obstet Gynaecol 1997; 104: 538–543.
  • 32. King RG, Gude NM, Di Lulio JL, Brennecke SP. Regulation of human placental fetal vessel tone. Role of nitric oxide. Reprod Fert Develop 1995; 7: 1407–1411.
  • 33. Shaamash AH, Elsnosy ED, Machlouf AM, Zakhari MM, İbrahim OA, El-Dien HM. Maternal and fetal nitric oxide (NO) concentration in normal pregnancy, pre-eclampsia and eclampsia. Int J Gynecol Obstet 2000; 68: 207–214.
  • 34. Choi JW, Im WM, Pai SH. Nitric oxide production increases during normal pregnancy and decreases in preeclampsia. Ann Clin Lab Sci 2002; 32: 257–263.
  • 35. Begüm S, Yamasaki M, Mochizuki M. Urinary levels of nitric oxide metabolites in normal pregnancy and preeclampsia. J Obstet Gynaecol Res 1996; 22: 551–559.
  • 36. Feldman PL, Stuehr DJ, Grifth OW, Fukuto JM. Mechanisms of mammalian nitric oxide biosynthesis. In: Weisman BA, Allon N, Shapira S, editors. Biochemical, Pharmacological and Clinical Aspects of Nitric Oxide. New York, NY, USA: Plenum Press; 1995. p. 14–20.
  • 37. Ademuyıwa O, Odusoga OL, Adebawo OO, Ugbaja RN. Endogenous antioxidant defences in plasma and erythrocytes of pregnant women during diferent trimesters of pregnancy. Acta Obstet Gyn Scan 2007; 86: 1175–1180.
  • 38. Djordjevic A, Spasic S, Galovic AJ, Djordjevic R, Lajsic GG. Oxidative stress in diabetic pregnancy: SOD, CAT and GPx activity and lipid peroxidation products. J Matern-Fetal Neo M 2004; 16: 367–372.
  • 39. Erişir M, Benzer F, Kandemir FM. Changes in the rate of lipid peroxidation in plasma and selected blood antioxidants before and during pregnancy ewes. Acta Vet Brno 2009; 78: 237–242.
  • 40. Öztabak K, Civelek S, Özpınar A, Burçak G, Esen F. The effects of energy restricted diet on the activities of plasma Cu-Zn SOD, GPx, CAT and TBARS concentrations in late pregnant ewes. Turk J Vet Anim Sci 2005; 29: 1067–1071.
  • 41. Cochrane CG. Cellular injury by oxidants. Am J Med 1991; 91: 23–30.
  • 42. Gutman LA, Flores-Sanchez MM, Diaz-Florez M, Hicks JJ. Presence of uterine peroxidase activity in the rat early pregnancy. Int J Biochem Cell B 2000; 32: 255–262.
  • 43. Novak Z, Kovacs L, Pal A, Pataki L, Varga SI, Matkovics B. Te antioxidant enzymes and lipid peroxidation in cord and maternal red blood cell haemolysates. Clin Chim Acta 1989; 180: 103–106.
  • 44. Biri A, Onan A, Devrim E, Babacan F, Kavutcu M, Durak İ. Oxidant status in maternal and cord plasma and placental tissue in gestational diabetes. Placenta 2006; 27: 327–332.
  • 45. Devrim E, Tarhan İ, Ergüder İB, Durak İ. Oxidant/antioxidant status of placenta, blood and cord blood samples from pregnant women supplemented with iron. J Soc Gynecol Investig 2006; 13: 502–505.
  • 46. Suhail M, Suhail S, Gubta BK, Bharat V. Malondialdehyde and antioxidant enzymes in maternal and cord blood and their correlation in normotensive and preeclamptic women. J Clin Med Res 2009; 1: 150–157.
  • 47. Wisdom SJ, Wilson R, McKillop JH, Walker JJ. Antioxidant systems in normal pregnancy and in pregnancy hypertension. Am J Obstet Gynecol 1991; 6: 1701–1705.
  • 48. Michelson AM, Pujet K, Durosay P, Bonneau JC, Ropartz C. Superoxide dismutase levels in human erythrocytes. In: Hayashi O, Asada K, editors. Biochemical and Medical Aspects of Active Oxygen. Tokyo: Japan Scientifc Society Press; 1977. p. 247.
  • 49. Kobayashi Y, Ishigame Y, Ishigame K, Usui T. Superoxide dismutase activity of human blood cells. In: Hayashi O, Asada K, editors. Biochemical and Medical Aspects of Active Oxygen. Tokyo: Japan Scientifc Society Press; 1977. p. 261.
  • 50. Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol 1979; 135: 372–376.
  • 51. Dubinina EE, Sofronova LN, Ramenskaia NP, Efmova LF, Petrova ZA. Status of the antioxidant system of erythrocytes in newborn infants in acute and chronic hypoxia. Vopr Med Khim 1989; 35: 56–59.
  • 52. Dede FS, Guney Y, Dede H, Koca C, Dilbaz B, Bilgihan A. Lipid peroxidation and antioxidant activity in patients in labor with nonreassuring fetal status. Eur J Obstet Gyn R B 2006; 124: 27–31.
  • 53. Uzar E, Yılmaz HR, Yılmaz M, Uz E, Yürekli VA, Dündar B, Koyuncuoğlu HR, Çömlekçi S. Efects of 50 Hz electric feld on malondialdehyde and nitric oxide levels in spinal cord of rats at prenatal plus postnatal period. Turk J Med Sci 2011; 41: 65–72.
  • 54. Padmini E, Rani UM. Lipid profle alterations and oxidative stress in patients with preeclampsia: role of black tea extract on disease management. Turk J Med Sci 2011; 41: 761–768.
  • 55. Çömlekçioğlu Ü, Yalın S, Ballı E, Berköz M. Ovariectomy decreases biomechanical quality of skin via oxidative stress in rat. Turk J Med Sci 2012; 42: 201–209.
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  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
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