Necdet SÜT, Elif Ezgi GÜREL, Selma Arzu VARDAR, Çetin Hakan KARADAĞ, Ruhan Deniz TOPUZ, Orkide PALABIYIK, Semra ÇALIŞKAN, Serap ÖZEN TOPÇU

Hemodynamic efects of atrial natriuretic peptide in ischemia-reperfusion injury that occurs afer exercise

Background/aim: Atrial natriuretic peptide (ANP) is known as a protective agent against ischemia-reperfusion injury for cardiomyocytes.We compared the hemodynamic efects of ANP and isatin, which is known as an ANP receptor blocker, in ischemia followed byreperfusion in exercised rat hearts with nonexercised ones.Materials and methods: Isolated hearts were perfused in 4 exercised (E) groups afer a running protocol for 5 days and 4 nonexercised(NE) groups. In the frst protocol, ANP was added to the perfusion solution before ischemia in an E and NE group. In the secondprotocol, diferent doses of isatin (0.1, 10, 100 µM/L) were added to the perfusion solution before ANP in 3 E and 3 NE groups. Lefventricular developed pressure (LVDP) and maximum and minimum rates of change in lef ventricular pressure (dP/dtmax and dP/dtmin) were recorded.Results: Higher LVDP and dP/dtmin values were observed in the E group than the NE group following addition of ANP before ischemia.Values of dP/dtmax were higher in the E group at the frst minute of reperfusion period. Hemodynamic diference was not observedbetween groups given the same amount of isatin before ANP.Conclusion: Tis study indicated that higher ANP concentrations before ischemia were more efective on the lef ventricle contractilityand relaxation functions in the hearts that were exposed to exercise.

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