Güleser GÖKTAŞ, Serdar DİLBAZ, Özgür ÇINAR, İskender KAPLANOĞLU, Gülnur TAKE KAPLANOĞLU, Cemile Merve SEYMEN

Is leptin receptor expression triggered in the case of embryo transfer to endometrium coculture?

Background/aim: A synchronized dialogue between maternal and embryonic tissues is required for successful implantation. Lowuterine receptivity is responsible for two-thirds of implantation failures and leptin is effective in the physiology of reproduction bybinding to specific receptors. In this study, we investigate leptin receptor expression in cases of embryo transfer to endometrial coculture.Materials and methods: Biopsy materials were taken from 20 females with indication for coculture application and were cultured in anappropriate medium after the epithelial cells were isolated. The grown cells were cultured in chamber slides as the first group. For thesecond group, day 3 embryo was added to chamber slides and the development was observed. The embryo was transferred 1 or 2 dayslater and other cells (after the transfer process) were used to form the second group. After fixation, immunohistochemical staining wasperformed with anti-leptin primary antibody.Results: Regarding the coculture without embryo transfer, moderate leptin receptor immunoreactivity was seen in the perinuclearregion and the cell membrane. Also, regarding the coculture with embryo transfer, moderate leptin receptor immunoreactivity was seenin the cytoplasm and strong leptin receptor immunoreactivity was seen in the cell membrane.Conclusion: Embryo transfer to endometrium coculture triggers leptin receptor expression.

PDF

___

1. Seymen CM, Elmas C. The effects of oxidative stress and some of the popular antioxidants on reproductive system: a mini review. Journal of Nutrition & Food Sciences 2016; 6: 2. doi: 10.4172/2155-9600.1000464
2. Garcia MD, Nera A, Briand L, Topie E, Bencharif D et al. In vitro fertilization (IVF). In: Recent Advances in In Vitro Fertilization. Telangana, India: Avid Science; 2016. pp. 2-46.
3. Kim J, Lee J, Kim SH, Jun JH. Coculture of preimplantation embryos with outgrowth embryos improves embryonic developmental competence in mice. Reproductive Sciences 2016; 23 (7): 913-923. doi: 10.1177/1933719115623641
4. Melnick AP, Murphy EM, Masbou AK, Sapra KJ, Rosenwaks Z et al. Autologous endometrial coculture biopsy: Is timing everything? Fertility and Sterility 2015; 104 (1): 104-109. doi: 10.1016/j.fertnstert.2015.04.026
5. Achache H, Revel A. Endometrial receptivity markers, the journey to success embryo implantation. Human Reproduction Update 2006; 12 (6): 731-746. doi: 10.1093/humupd/dml004
6. Altmae S, Martinez-Conejero JA, Salumets A, Simon C, Horcajadas JA et al. Endometrial gene expression analysis at the time of embryo implantation in women with unexplained infertility. Molecular Human Reproduction 2010; 16 (3): 178- 187. doi: 10.1093/molehr/gap102
7. Santos ED, Serazin V, Morvan C, Torre A, Wainer R et al. Adiponectin and leptin systems in human endometrium during window of implantation. Fertility and Sterility 2012; 97 (3): 771-778. doi: 10.1016/j.fertnstert.2011.12.042
8. Cavagna M, Mantese JC. Biomarkers of endometrial receptivity-A review. Placenta 2003; 24 (Suppl. B): 39-47.
9. Ledee N, Chaouat G, Serazin V, Lombroso R, Dubanchet S et al. Endometrial vascularity by three-dimensional power Doppler ultrasound and cytokines: a complementary approach to assess uterine receptivity. Journal of Reproductive Immunology 2008; 77 (1): 57-62. doi: 10.1016/j.jri.2007.07.006
10. Gonzalez RR, Simon C, Caballero-Campo P, Norman R, Chardonnens D et al. Leptin and reproduction. Human Reproduction Update 2000; 6 (3): 290-300. https://doi. org/10.1093/humupd/6.3.290
11. Melnick AP, Spandorfer SD. Embryo coculture: a review. Journal of Epidemiological Research 2016; 2 (1): 15-19. doi: 10.5430/jer.v2n1p15
12. Barmat LI, Worrilow KC, Paynton BV. Growth factor expression by human oviduct and buffalo rat liver coculture cells. Fertility and Sterility 1997; 67 (4): 775-779
13. Fukui Y, McGowan LT, James RW, Pugh PA, Tervit HR. Factors affecting the in-vitro development to blastocysts of bovine oocytes matured and fertilized in-vitro. Journal of Reproduction and Fertility 1991; 92 (1): 125-131
14. Choi YS, Oh HK, Choi JH. Expression of adiponectin, leptin, and their receptors in ovarian endometrioma. Fertility and Sterility 2013; 100 (1): 135-141. doi: 10.1016/j. fertnstert.2013.03.019
15. Gonzalez RR, Caballero-Campo P, Jasper M, Mercader A, Devoto L et al. Leptin and leptin receptor are expressed in the human endometrium and endometrial leptin secretion is regulated by the human blastocyst. The Journal of Clinical Endocrinology & Metabolism 2000; 85 (12): 4883-4888. doi: 10.1210/jcem.85.12.7060
16. Cervero A, Horcajadas JA, Domingues F, Pellicer A, Simon C. Leptin system in embryo development and implantation: a protein in search of a function. Reproductive BioMedicine Online 2005; 10 (2): 217-223
17. Gosman GG, Katcher HI, Legro RS. Obesity and the role of gut and adipose hormones in female reproduction. Human Reproduction Update 2006; 12 (5): 585-601. doi: 10.1093/ humupd/dml024
18. Chehab FF, Lim ME, Lu R. Correction of the sterility defect in homozygous obese female mice by treatment with the human recombinant leptin. Nature Genetics 1996; 12 (3): 318-320. doi: 10.1038/ng0396-318
19. Mounzih K, Lu R, Chehab FF. Leptin treatment rescues the sterility of genetically obese ob/ob males. Endocrinology 1997; 138 (3): 1190-1193. doi: 10.1210/endo.138.3.5024
20. Kawamura K, Sato N, Fukuda J, Kodama H, Kumagai J et al. Leptin promotes the development of mouse preimplantation embryos in vitro. Endocrinology 2002; 143 (5): 1922-1931. doi: 10.1210/endo.143.5.8818
21. Yang YJ, Cao YJ, Bo SM, Peng S, Liu WM et al. Leptindirected embryo implantation: Leptin regulates adhesion and outgrowth of mouse blastocyts and receptivity of endometrial epithelial cells. Animal Reproduction Science 2006; 92 (1-2): 155-167. doi: 10.1016/j.anireprosci.2005.05.019
22. Zhang Y, Proenca R, Maffei M, Barone M, Leopold L et al. Positional cloning of the mouse obese gene and its human homologue. Nature 1994; 372 (6505): 425-432. doi: 10.1038/372425a0