Gülseren BAĞCI, Göksemin DEMİR, İbrahim AÇIKBAŞ, Hüseyin BAĞCI

In Vitro Testing for Genotoxicity of 4-CPA by Sister Chromatid Exchange in Human Lymphocyte Culture

Certain phenoxyacetate derivatives used as plant growth regulators act as plant hormones. 2,4-D dichlorophenoxyacetic acid and 4-chlorophenoxyacetic acid are widely used in vegetable growing, especially tomatoes, in our region. These phenoxyacetic acid compounds are a potential hazard for the environment. There are conflicting results and inconsistent data about these herbicides in the literature. This is the first report of genotoxicity of 4-CPA evaluated by sister chromatid exchange in human lymphocyte culture. No significant change was found in sister chromatid exchange frequency at the tested 4-CPA concentration.

Anahtar Kelimeler:

4-CPA, Phenoxy herbicides, genotoxicity, sister chromatid exchange, tomato

In Vitro Testing for Genotoxicity of 4-CPA by Sister Chromatid Exchange in Human Lymphocyte Culture

Keywords:

4-CPA, Phenoxy herbicides, genotoxicity, sister chromatid exchange, tomato,

PDF

___

Topaktafl M, Rencuzoğlu E, Ila HB et al. Chromosome aberration and sister chromatid exchange in workers of the iron and steel factory of İskenderun, Turkey. Teratogenesis Carcinog Mutagen 22: 411-423, 2002.
See RH, Dunn BP, Sun RH. Clastogenic activity in urine of workers occupationally exposed to pesticides. Mutat Res 241: 251-259, 1990.
Kaya B, Yanikoglu A, Marcos R. Genotoxicity studies on the phenoxyacetates 2,4-D and 4-CPA in the Drosophila wing spot test. Teratogenesis Carcinog Mutagen 19: 305-312, 1999. 4. Mustonen R, Kangas J, Vuojolahti P et al. Effects of phenoxyacetic acids on the induction of chromosome aberrations in vitro and in vivo. Mutagenesis 4: 241-245, 1986.
Lilienfeld DE. Gallo MA. 2.4-D, 2,4.5-T and 2.3,7.8-TCDD: An overview. Epidemiol Rev 11: 28-58, 1989.
Arias E. Cytogenetic and cytokinetic effects of 2,4-D on B lymphocytes during development of the chick embryo. In Vitro Toxicol 8: 65-69, 1995. 7. Arias E. Effects of the phenoxy herbicide MCPA on SCE frequency and cell kinetics in developing chick embryos. Ecotoxicol Environ Safety 33: 25-29, 1996. 8. Kale GP, Petty BT Jr., Walker S et al. Mutagenicity testing of nine herbicides and pesticides currently used in agriculture. Environ Molec Mutagen 25: 148-153, 1995.
Vogel E, Chandler JLR. Mutagenicity testing of cyclamate and some pesticides in Drosophila melanogaster. Experientia 30: 621- 623, 1974.
Magnusson J, Ramel C, Eriksson A. Mutagenic effects of chlorinated phenoxyacetic acids in Drosophila melanogaster. Hereditas 87: 121-123, 1977.
Seiler JP. The genetic toxicology of phenoxy acids other than 2,4,5-T. Mutation Res 55: 197-226, 1978.
Moriya M, Ohta T, Watanabe K et al. Further mutagenicity studies on pesticides in bacterial reversion assay systems. Mutation Res 116: 185-216, 1983.
Sorsa M. Monitoring of sister chromatid exchange and micronuclei as biological endpoints. IARC Sci Publ 59: 339-349, 1984.
Sarto F, Tomanin R, Giacomelli L et al. Evaluation of chromosomal aberrations in lymphocytes and micronuclei in lymphocytes, oral mucosa and hair root cells of patients under antiblastic therapy. Mutat Res 228: 157-169, 1990.
Gomez-Arroyo S. Noriega-Aldana N, Osorio A et al. Sister chromatid exchange analysis in a rural population of Mexico exposed to pesticides. Mutat Res 281: 173-179, 1992.
Motykiewicz G, Michalska J, Pendzich J et al. A cytogenetic study of men environmentally and occupationally exposed to airborne pollutants. Mutat Res 280: 253-259, 1992.
Rubes J, Borkovec L, Horinova Z et al. Cytogenetic monitoring of farm animals under conditions of environmental pollutions. Mutat Res 283: 199-210, 1992.
Kasuba V, Rozgaj R, Sentija K. Cytogenetic changes in subjects occupationally exposed to benzene. Chemosphere 40: 307-310, 2000.
Kaya M, Koçer İ, Öztafl S. Comparison of sister chromatid exchange frequency between two distinct forms of anterior uveitis. Turk J Med Sci 30: 71-76, 2000.
Perry P. Wolff S. New Giemsa method for the differential staining of sister chromatids. Nature 251: 156-158, 1974.
Albertini RJ, Atland K, Carrano AV et al. International programme on chemical safety guidelines for the study of genetic effects in human populations. Environmental Health Criteria 46-1984. World Health Organization, Geneva. 1985.
Atmaca M, Bağcı H, Açıkbafl İ et al. Sister chromatid exchange frequency in lymphocytes cultured from cotton gin workers. Turk J Med Sci 34: 247-250, 2004.
Biri A, Civelek E, Karahalil B et al. Assessment of DNA damage in women using oral contraceptives. Mutat Res 521: 113-119, 2002.
Margolin BH, Shelby MD. Sister chromatid exchanges: a reexamination of the evidence for sex and race differences in humans. Environ Mutagen 7 (Suppl 4): 63-72, 1985.
Wulf HC, Niebuhr E. Different sister chromatid exchange rates in XX and XY cells of a pair of human chimeric twins. Cytogenet Cell Genet 39: 105-108, 1985.
Atabey N, Güdener S, Paralı F et al. Clinical, Biochemical and genetic (SCE Frequency) studies of pesticide sprayers. Tr J of Medical Sciences Sci 26: 399-402, 1996.