A New Diagnostic System in Cancer Research: Bioluminescent Imaging (BLI)
Bioluminescent imaging (BLI) is particularly well suited for imaging small animals, and it can be readily used by scientists who are already routinely using the luciferase gene as a reporter in cell-based assays. This strategy relies on ATP and an O2 dependent photochemical reaction between luciferin and luciferase, resulting in the release of photons from only live cells. In BLI, the intensity of the light detected by the device is proportional to the intensity of light emitted and the relationship is quantitative. This study was performed to determine the sensitivity of BLI for the imaging of lung metastasis and breast cancer models in addition to primary flank tumors. A549 lung cancer and MDA-MB 231 breast cancer cell lines were transfected with a lentiviral construct containing the luciferase gene and transfected cells were used as xenograft animal models. BLI was used to monitor tumor development and growth. Noninvasive bioluminescent imaging in vivo provides easy visualization of the tumor size and location so that imaging results can be used as an indicator of the treatment effectiveness, potentially accelerating the optimization of cancer treatment protocols.
A New Diagnostic System in Cancer Research: Bioluminescent Imaging (BLI)
Bioluminescent imaging (BLI) is particularly well suited for imaging small animals, and it can be readily used by scientists who are already routinely using the luciferase gene as a reporter in cell-based assays. This strategy relies on ATP and an O2 dependent photochemical reaction between luciferin and luciferase, resulting in the release of photons from only live cells. In BLI, the intensity of the light detected by the device is proportional to the intensity of light emitted and the relationship is quantitative. This study was performed to determine the sensitivity of BLI for the imaging of lung metastasis and breast cancer models in addition to primary flank tumors. A549 lung cancer and MDA-MB 231 breast cancer cell lines were transfected with a lentiviral construct containing the luciferase gene and transfected cells were used as xenograft animal models. BLI was used to monitor tumor development and growth. Noninvasive bioluminescent imaging in vivo provides easy visualization of the tumor size and location so that imaging results can be used as an indicator of the treatment effectiveness, potentially accelerating the optimization of cancer treatment protocols.
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- Contag CH, Ross BD. It’s not just about anatomy: In vivo bioluminescence imaging as an eyepiece into biology. JMRI 16: 378-87, 2002.
- Scatena CD, Hepner MA, Oei YA et al. Imaging of bioluminescent LNCaP-luc-M6 tumors: a new animal model for the study of metastatic human prostate cancer. Prostate 59: 292-303, 2004.
- Sarraf-Yazdi S, Mi J, Dewhirst MW et al. Use of in vivo bioluminescence imaging to predict hepatic tumour burden in mice. J Surg Res 120: 249-55, 2004.
- Soling A, Rainov NG. Bioluminescence imaging in vivo – application to cancer research. Expert Opin Biol Ther 3: 1163-72, 2003.
- Jenkins DE, Oei Y, Hornig YS et al. Bioluminescent imaging (BLI) to improve and refine traditional murine models of tumor growth and metastasis. Clin Exp Metastasis 20: 733-44, 2003.
- Doyle TC, Burns SM, Contag CH. In vivo bioluminescence imaging for integrated studies of infection. Cellular Microbiology 6: 303- 317, 2004.
- Blasberg RG. In vivo molecular-genetic imaging: multi-modality nuclear and optical combinations. Nucl Med Biol 30: 879-88, 2003.
- Choy G, O’Connor S, Diehn FE et al. Comparison of noninvasive fluorescent and bioluminescent small animal optical imaging. Biotechniques 35: 1022-6, 1028-30, 2003.
- Nogawa M, Yuasa T, Kimura S et al. Monitoring luciferase-labeled cancer cell growth and metastasis in different in vivo models. Cancer letters 217: 243-253, 2005.
- Caceres G, Zhu XY, Jiao JA et al. Imaging of luciferase and GFP- transfected human tumours in nude mice. Luminescence Jul-Aug; 18: 218-23, 2003.
- Edinger M, Cao YA, Hornig YS et al. Advancing animal models of neoplasia through in vivo bioluminescence imaging. Eur J Cancer, Nov; 38: 2128-36, 2002.