Yousef MOTAHARINIA, Mohammad Ali REZAEE, Mohammad Saleh HAZHIR, Reza SHAPOUR, Mohammad Saied HAKHAMANESHI, Ahmad RASHIDI, Mohammad Reza RAHMANI, Farid ZANDI

Evaluation of the antibacterial activity of Zataria multiflora Boiss., Rhus coriaria L. (sumac), Mentha piperita L., and Ocimum basilicum L. extracts on Brucella strains isolated from brucellosis patients

Since brucellosis has been called a chronic illness and treatment of patients infected with this bacterium has not been successful, the present study aim ed to evaluate the effects of several medicinal plant extracts on clinical Brucella strains. Materials and methods: First, Brucella strains were isolated from brucellosis patients. Then an antibiotic resistance assay was performed for these strains, while the antibacterial activity of the above-mentioned extracts was evaluated. Results: After performing accurate evaluations, the obtained results showed that all of the isolated Brucella strains were sensitive to tetracycline, doxycycline, and gentamicin, and the rates of antibiotic resistance to rifampin and streptomycin were 83.3% and 11.1%, respectively. The mean zone of growth inhibition for Zataria multiflora Boiss. was >=28.77 mm, Rhus coriaria L. (sumac) was 22.55 mm, Mentha piperita L. was >=7.5 mm, and the gentamicin disk was >=30 mm. The mean minimum inhibitory concentration (MIC) for Z. multiflora was 1237 mg/mL, and it was 3255.2 mg/mL for sumac, and 5642 mg/mL for M. piperita L. The minimum bactericidal concentration (MBC) rate for these herbs was 5900 mg/mL, 9027 mg/mL, and 12152 mg/mL, respectively. Furthermore, no anti-Brucella activity was observed for Ocimum basilicum L. Conclusion: The results obtained in this study prove that Z. multiflora extracts show high anti-Brucella activity and it can be used for better treatment of brucellosis.

Anahtar Kelimeler:

Zataria multiflora Boiss., Rhus coriaria L., Mentha piperita L., Ocimum basilicum L., antibacterial activity, Brucella

Evaluation of the antibacterial activity of Zataria multiflora Boiss., Rhus coriaria L. (sumac), Mentha piperita L., and Ocimum basilicum L. extracts on Brucella strains isolated from brucellosis patients

Keywords:

Zataria multiflora Boiss., Rhus coriaria L., Mentha piperita L., Ocimum basilicum L., antibacterial activity, Brucella,

PDF

___

Lim M, Rickman L. Brucellosis. Infect Dis Clin Pract 2004; 12: 7-14.
Abramson O, Rosenvasser Z, Block C, Dagan R. Detection and treatment of brucellosis by screening a population at risk. Pediatr Infect Dis J 1991; 10: 434-438.
Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos E. Th e new global map of human brucellosis. Lancet Infect Dis 2006; 6: 91-99.
Moradi G, Esmaiel Nasab N, Ghaderi E, Majidpour S M, Salimzadeh H. Brucellosis in Kurdistan Province from 1997 to 2003. Annals of Alquds Medicine 2006; 2: 32-37.
Franco PM, Mulder M, Gilman RH, Smits HL. Human brucellosis. Lancet Infect Dis 2007; 7: 775-86.
Akova M, Gur D, Livermore DM, Kocagoz T, Akalin HE. In vitro activities of antibiotics alone and in combination against Brucella melitensis at neutral and acidic pHs. Antimicrob Agents Chemother 1999; 43: 1298-300.
Roushan MR, Gangi SM, Ahmadi SA. Comparison of the effi cacy of two months of treatment with co-trimoxazole plus doxycycline vs. co-trimoxazole plus rifampin in brucellosis. Swiss Med Wkly 2004; 134: 564-8.
Hashemi SH, Keramat F, Ranjbar M, Mamani M, Frzam A, Jamal-omidi S. Osteoarticular complications of brucellosis in Hamedan, an endemic area in the west of Iran. Int J Infect Dis 2007; 11: 496-500.
Gangoue PJ, Koulla SHS, Ngassam P, Adiogo D, Ndumbe P. Antimicrobial activity against gram negative bacilli from Yaounde Central Hospital, Cameroon. Afr Health Sci 2006; 6: 232-35.
Rayne S, Mazza G. Biological activities of extracts from sumac (Rhus spp.): a review. Plant Foods Hum Nutr 2007; 62: 165- 175.
Fazeli MR, Amin G, Attari MM, Ashtiani H, Jamalifar H, Samadi N. Antimicrobial activities of Iranian sumac and Avishane shirazi (Zataria multifl ora) against some food-borne bacteria. Food Control 2007; 646-649.
Arumugam P, Murugan R, Subathra M, Ramesh A. Superoxide radical scavenging and antibacterial activities of diff erent fractions of ethanol extract of Mentha spicata (L.). Med Chem Res 2010; 19: 664-673.
Sokovic M, Marin PD, Brkic D, Griensven LV. Chemical composition and antibacterial activity of essential oils of ten aromatic plants against human pathogenic bacteria. Food 2007; 1-7.
Mathur A, Purohit R, Mathur D, Dua VK. Pharmacological investigation of methanol extract of Mentha piperita L. roots on the basis of antimicrobial, antioxidant and anti-infl ammatory properties. Der Pharmacia Sinica 2011; 2: 208-216.
Budka D, Ahmed Khan N. Th e eff ect of Ocimum basilicum, Th ymus vulgaris, Origanum vulgare essential oils on Bacillus cereus in rice-based foods. EJBS 2010; 2: 17-20.
Rattanachaikunsopon P, Phumkhachorn P. Antimicrobial ac- tivity of basil (Ocimum basilicum) oil against S almonella enter- itidis in vitro and in food. Biosci Biotechnol Biochem 2010; 74: 1200-1204.
Kazemi B, Yousefi Namin SA, Dowlatshahi M, Bandepour M, Kafi lzadeh F. Detection of Brucella by peripheral blood PCR and comparison with culture and serological methods in suspected cases. Iranian J Publ Health 2008; 37: 96-102.
Khosravi A, Abassi E, Alavi SM. Isolation of Brucella Melitensis and Brucella Abortus from brucellosis patients by conventional culture method and polymerase chain reaction technique. Pak J Med Sci 2006; 22: 396-400.
Mitka S, Anetakis C, Souliou E, Diza E, Kansouzidou A. Evaluation of diff erent PCR assays for early detection of acute and relapsing brucellosis in humans in comparison with conventional methods. J Clin Microbiol 2007; 1211-1218.
Cekovska Z, Petrovska M, Jankoska G, Panovski N, Kaft andzieva A. Isolation, identifi cation and antimicrobial susceptibility of Brucella blood culture isolates. Contributions Sec Biol Med Sci MASA 2010; 117-132.
Motamedi H, Darabpour E, Gholipour M, Seyyed Nejad SM. In vitro assay for the anti-brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis. J Zhejiang Univ Sci B 2010; 11: 506-11.
Vrioni G, Pappas G, Priavali E, Gartzonika C, Levidiotou S. An eternal microbe: Brucella DNA load persists for years aft er clinical cure. Clin Infect Dis 2008; 46: e131-6.
Baykam N, Esener H, Ergönül O, Eren S, Celikbas AK, Dokuzoguz B. In vitro antimicrobial susceptibility of Brucella species. Int J Antimicrobial Agents 2004; 23: 405-407.