Effects of phenolic acids on gastrointestinal smooth muscle complications in streptozotocine-induced diabetic rats

To identify the effects of antioxidants on gastrointestinal smooth muscle complications in 6-week streptozotocine (STZ)-induced diabetic rats. Materials and methods: The following steps were carried out: 1. Three weeks after STZ injection, saline and 10 mg/kg p-OH benzoic, protocatechic, and gallic acids were separately administered to the rat groups every day for 3 weeks. 2. The responses of the fundus and ileum to agonists were recorded using isolated organ bath experiments. Results: Diabetic smooth muscle complications developed in the diabetic rats. The responses of the ileum to acetylcholine increased whereas the responses of the fundus to serotonine decreased in the diabetic rats. The test drugs inhibited and promoted but did not affect the smooth muscle complications in the diabetic rats, and sometimes they affected the smooth muscle activity, which did not change with diabetes. Conclusion: The antioxidants used elicited pro-oxidant activation and/or exhibited effects independent of diabetic complications of gastrointestinal smooth muscles in the experimental conditions applied.

Anahtar Kelimeler:

Key words: Diabetes mellitus, gastrointestinal tract complication, p-OH benzoic acid, protocatechic acid, gallic acid

Effects of phenolic acids on gastrointestinal smooth muscle complications in streptozotocine-induced diabetic rats

Keywords:

Key words: Diabetes mellitus, gastrointestinal tract complication, p-OH benzoic acid, protocatechic acid, gallic acid,

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Aydin S, Ozturk Y, Altan VM, Yildizoglu-Ari N, Ozcelikay AT. Eff ect of insulin treatment on smooth muscle calmodulin levels in rats with long-term streptozotocine diabetes. Mol Cell Endocrinol 1996; 116: 67-71.
Ozturk Y, Altan VM, Yıldizoglu-Ari N. Eff ects of experimental diabetes and insulin on smooth muscle functions. Pharmacol Rev 1996; 48: 69-112,
Edwards JL, Vincent AM, Cheng HT, Feldman EL. Diabetic neuropathy: Mechanisms to management. Pharmacol Th er 2008; 120: 1-34.
El-Salhy M, Zachrisson S, Spångéus A. Abnormalities of small intestinal endocrine cells in non-obese diabetic mice. J Diabetes Complicat 1998. 12: 215-223.
Valensi P, Devehat CL, Richard JL, Farez C, Khodabandehlou T, Rosenbloom R et al.. A multicenter, double-blind, safety study of QR-333 for the treatment of symptomatic diabetic peripheral neuropathy: A preliminary report. J Diabetes Complicat 2005; 19: 247-253.
Hsu FL, Chen YC, Cheng JT. Caff eic acid as active principle from the fruit of Xanthium strumarium to lower plasma glucose in diabetic rats. Planta Med 2000; 66: 228-230.
Mukherjee PK, Maiti K, Mukherjee K, Houghton PJ. Leads from Indian medicinal plants with hypoglycemic potentials. J Ethnopharmacol 2006; 106: 1-28.
Peyrat-Maillard MN, Bonnely S, Berset C. Determination of the antioxidant activity of phenolic compounds by coulometric detection. Talanta 2000; 51: 709-716.
Oh TK, Li MZ, Kim ST. Gene therapy for diabetes mellitus in rats by intramuscular injection of lentivirus containing insulin gene. Diabetes Res Clin Pract 2006; 71: 233-240.
Kugino K, Suzuki I, Kugino M. Studies on Ach-induced contraction of ileum in rats. Nutr Res 1998; 18: 1879-1888.
Santos MRV, Marchioro M, Antoniolli AR. Lead eff ects on non-adrenergic noncholinergic relaxations in the rat gastric fundus. Toxicol In Vitro 2006; 20: 38-42.
Lincoln J, Bokor JT, Crowe R, Griffi th SG, Haven AJ, Burnstock G. Myenteric plexus in streptozotocin-treated rats: Neurochemical and histochemical evidence for diabetic neuropathy in the gut. Gastroenterology 1984; 86: 654-661.
Nowak TV, Harrington B, Kalbfl eisch JH, Amatruda JM. Evidence for abnormal cholinergic neuromuscular transmission in diabetic rat small intestine. Gastroenterology 1986; 91: 124-132.
Altan VM, Yildizoglu N, Ozturk Y. Decreased gastro-intestinal responses to certain agonists in streptozotocin-and alloxan- diabetic rats in vitro. Pharmacology 1987; 34: 143-148.
Aihara K, Sakai Y. Hyperreactivity of contractile response in gastric fundus smooth muscle from rats with diabetes induced by streptozotocine. Arch Int Pharmacodyn Th er 1989; 302: 220-231.
Pinna C, Bolego C, Puglisi L. Eff ect of substance P and capsaicin on stomach fundus and ileum of streptozocin-diabetic rats. Eur J Pharmacol 1995; 276: 61-69.
Koc E, Ayaz M, Saran Y, Turan B. Relationship between structural alterations of ileal smooth muscle and altered responses to acetylcholine in streptozotocin-induced diabetic rats. T Clin J Med Res 2002; 20: 65-71.
Bentley PJ. Endocrine pharmacology: physiological basis and therapeutic applications. New York, Cambridge University Press, 1980.
Ozturk Y, Yildizoglu-Ari N, Altan VM, Karasu C. Altered responses to calcium and trifl uoperazine in K+-depolarized duodenum from alloxan-diabetic rats. Pharmacol Res 1987; 19: 597-608.
Barada K, Okolo C, Field M, Cortas N. Na++,K+-ATPase in diabetic rat small intestine. Changes at protein and mRNA levels and role of glucagons. J Clin Invest 1994; 93: 2725-2731
Soulié ML, Cros G, Serrano JJ, Bali JP. Impairment of contractile response to carbachol and muscarinic receptor coupling in gastric antral smooth muscle cells isolated from diabetic streptozotocin-treated rats and db/db mice. Mol Cell Biochem 1992; 109: 185-188.
Bijender S, Harish D, Rishi S, Patil BM. Eff ect of vitamin E on the impaired gastrointestinal activity of streptozotocin induced diabetic rats. Indian J Pharmacol 2003; 35: 186-187.
Karasu C, Dewhurst M, Stevens EJ, Tomlinson DR. Eff ects of anti-oxidant treatment on sciatic nerve dysfunction in streptozotocin-diabetic rats; comparison with essential fatty acids. Diabetologia 1995; 38: 129-134.
Park S, Lee S. Antioxidant and prooxidant properties of ascorbic acid on hepatic dysfunction induced by cold ischemia/ reperfusion. Eur J Pharmacol 2008; 580: 401-406.
Gil-Longo J, González-Vázquez C. Vascular pro-oxidant eff ects secondary to the autoxidation of gallic acid in rat aorta. J Nutr Biochem 2009; 21: 304-309.
Sugihara N, Arakawa T, Ohnishi M, Furuno K. Anti-and pro-oxidative eff ects of fl avonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with alpha-linolenic acid. Free Radic Biol Med 1999; 27: 1313-1323.
Prat ML, López-Gonzálvez A, Ruiz MA, Barbas C. Ultrasound- assisted extraction for rapid determination of Zn, Cu, Fe, Mg and Mn in liver of diabetic rats under diff erent antioxidant treatments. J Pharmaceut Biomed 2009; 49: 1040-1044.
Oster MH, Uriu-Hare JY, Trap CL, Stern JS, Keen CL. Dietary macronutrient composition infl uences tissue trace element accumulation in diabetic Sprague-Dawley rats. Proc Soc Exp Biol Med 1994; 207: 67-75.
Zago MP, Oteiza PJ. Th e antioxidant properties of zinc: Interactions with iron and antioxidants. Free Radic Biol Med 2001; 31: 266-274.
Brandao-Neto J, Silva CAB, Rezende AA, Almeida MG, Sales VSP, Marchini JS. Zinc pharmacokinetics in insulin-dependent diabetes mellitus patients aft er oral zinc tolerance test. Nutr Res 2003; 23: 141-150.