Ali Aslan DÖNMEZ, Nurcan BEKTAŞ, Rana ARSLAN, Zeynep BOR, Sevda PIRILDAR

Antinociceptive, antiinflammatory, and antioxidant activities of the ethanol extract of Crataegus orientalis leaves

Amaç: Crataegus (alıç) bitkisinin türlerinin yaprak, çiçek ve meyvelerinden hazırlanan ekstrelerinin ilaç olarak kullanımı çok eski zamanlara dayanmaktadır. Alıcın yararlı terapötik etkilerinin antioksidan içeriğinden kaynaklandığı ileri sürülmektedir. Bu çalışmada Crataegus orientalis yapraklarının etanol ekstresinin in vitro antioksidan aktivitesinin belirlenmesi ve olası antinosiseptif, ve antiinflamatuvar etkilerinin araştırılması amaçlanmıştır. Yöntem ve gereç: Ekstrenin bu etkilerinin değerlendirilmesi için hot-plate, tail-immersion, kıvranma ve karragenin ile indüklenen pençe ödem testleri kullanılmıştır. Ekstrenin antioksidan aktivitesi 2,2-difenil-1-pikrilhidrazil ve β-karotenlinoleik asit sistemi kullanılarak tayin edilmiştir. Antinosiseptif ve antiinflamatuvar etkilerin belirlenmesi için uygulanan testlerde ekstrenin 50, 100 ve 200 mg/kg olarak hazırlanan dozları intraperitoneal olarak verilmiştir. Bulgular: Hot-plate testinde 100 ve 200 mg/kg dozlarında antinosiseptif etkide anlamlılık gözlenmiştir. Tail-immersion testinde tüm dozlarda görülen antinosiseptif etki kontrole göre anlamlılık göstermiştir. Kıvranma testinde ise doz arttıkça kıvranma sayılarında önemli bir düşüş görülmüştür. Antiinflamatuvar etki testinden elde edilen sonuçlar doza bağımlı bir etkinlik göstermiştir. Yapılan in vivo testlerde ekstrenin antioksidan aktiviteye sahip olduğu görülmüştür. Sonuç: Deneylerden elde edilen bulgulara göre, C. orientalis etanol ekstresinin antinosiseptif, antiinflamatuvar ve antioksidan aktivitelerinin dikkate değer olduğu sonucuna varılmıştır.

Crataegus orientalis yapraklarının etanol ekstresinin antinosiseptif, anti-inflamatuvar ve antioksidan etkileri

Aim: To determine the in vitro antioxidant activity and to investigate the possible antinociceptive and antiinflammatory effects of the ethanol extract of Crataegus orientalis leaves. The medicinal use of extracts prepared from the leaves, flowers, and fruits of Crataegus (hawthorn) dates back to ancient times. It has been proposed that its antioxidant constituents account for these benefi cial therapeutic eff ects. Materials and methods: To evaluate these effects, the hot-plate, tail-immersion, writhing, and carrageenan-induced edema tests were used. Moreover, the antioxidant activity of the extract was determined using the 2,2-diphenyl-1- picrylhydrazyl and β-carotene-linoleic acid assay systems. In the tests that were used to determine antinociceptive and antiinfl ammatory effects, the extract was administered in dose ranges of 50, 100, and 200 mg/kg, intraperitoneally. Results: In the hot-plate test, the doses of 100 and 200 mg/kg exerted signifi cant antinociceptive effects. In the tailimmersion test, antinociceptive effects were significant in all of the doses. In the writhing test, as the dose increased, a significant decrease in the writhing responses was observed. The antiinfl ammatory test results showed a dose-dependent antiinflammatory activity. In vivo tests also showed the extract as possessing antioxidant activity. Conclusion: According to the experimental findings, the ethanol extract of C. orientalis displays remarkable antinociceptive, antiinflammatory, and antioxidant activities.

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