Hayri ERKOL, Doğan Yeşim ALAKOÇ, Cüneyt ELMA, Aysun ÇETİNYÜREK, Zerrin ERKOL, Bora BÜKEN, Ayşim TUĞ

Allele distribution data for 16 short tandem repeat loci in Bolu

To examine the short tandem repeat (STR) data of Bolu population and compare the data with previously published population studies and with the data of a neighboring province, Düzce (a former district of Bolu), which became a province after the earthquake in 1999. Materials and methods: Blood samples were taken from 175 unrelated individuals. DNA was isolated using a DNA Kit and the amplification was performed using an AmpFlSTR Identifier kit. Genotyping was carried out by an ABI Prism 310 genetic analyzer by using a reference ladder. Several parameters, such as allele frequencies, Hardy-Weinberg equilibrium, power of exclusion, power of discrimination, pairwise comparison, were calculated and correction test was used to confirm significant differences found in the comparative analysis. Results: According to their power of exclusion and power of discrimination values, the most discriminating loci were D18S51 and D2S1338 whereas TPOX appears to be the least. The most discriminating loci and paternity index were found to be different in Bolu and its former district, Düzce, which is an interesting result. Conclusion: The results indicate the importance of local population studies, because in regions where migration occurs and marriages between members of different ethnic groups are not socially acceptable, genetic data are affected.

Anahtar Kelimeler:

Key words: DNA analysis, short tandem repeats, population genetics, Bolu, Turkey

Allele distribution data for 16 short tandem repeat loci in Bolu

Keywords:

Key words: DNA analysis, short tandem repeats, population genetics, Bolu, Turkey,

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Iwasa M, Wiegand P, Rand S, Schürenkamp M, Atasoy S, Brinkmann B. Genetic variation at five STR loci in subpopulations living in Turkey. Int J Legal Med. 1997; 110: 170- 172.
Filoglu G, Abaci-Kalfoglu E, Atasoy S. Allele frequencies for 10 STR loci in Istanbul (Turkey) population J Forensic Sci 2002; 4: 909-901.
Çerkezi AB, Altunçul DH, Yükseloğlu H, Abaci-KalfoğluE, Atasoy S. Allele frequencies for three STR population of Kosovo. J Forensic Sci 2002; 47: 908.
Çakır AH, Celebioglu A, Altunbaş S. STR data for the AmpFℓSTR Plus from Marmara region of Turkey J Forensic Sci. 2002; 127: 240-242.
Çakır AH, Çelebioglu A, Altunbaş, Yardımcı E. Allele frequencies for 15 STR loci in Van-Ağrı districts of the Eastern Anatolia region of Turkey. Forensic Sci Int 2003; 135: 60-63.
Shimada I,Rand S,Brinkmann B, Hohoff C. Kurdish population data for 11 STR loci (ACTBP2, CSF1PO, FGA,THO1,TPOX, vWA, D3S1358, D5S818, D7S820, D13S317 and D21S11) Int J Legal Med, 2002; 116: 310-303.
Salem RY, Mawieh MH, Ali ZE, Adnan SJ. African Jordanian population genetic database on fifteen short tandem repeat genetic loci Croat Med J 2005; 4: 587-592.
Skitsa I, Salas A, Lareu MV , Carracedo A. STR-CODIS typing in Greece. Forensic Sci Int 2003; 137:104–106.
Shepard EM, Herrera RJ. Iranian STR variation at the fringes of biogeographical demarcation Forensic Sci Int. 2006; 158: 140- 148.
Kornienko IV, Vodolazhsky DI, Ivanov PL. Genetic variation of the nine Profiler Plus loci in Russians Int J Legal Med 2002; 116: 309–311.
Budowle B,Chakraborty R. Population variation at the CODIS core short tandem repeat loci in Europeans, Legal Medicine 2001; 3:29-33.