Turgay ŞİŞMAN, MUHAMMED ATAMANALP, Hatice DANE, Şeymanur ADİL

The Toxicity of 10W-40 Motor Oil on Zebrafish Early Life Stages

Bu çalışmada 10W-40 motor yağının gelişimsel toksisitesi zebra balığı (Danio rerio) embriyolarında araştırılmıştır. Fertilizasyondan 1.5 saat sonra döllenmiş balık yumurtaları motor yağının sulandırılmış haline maruz bırakılmış (1.25, 2.5, 5.0,10.0, 20.0 and 40.0 v/v) ve maruziyet 72 saat sürdürülmüştür. Motor yağının zebra balığı embriyolarında sebep olduğu gelişimsel toksisitenin morfolojik belirtileri stereomikroskop altında takip edilmiştir. Motor yağının yüksek konsantrasyonları akut ölümlere yol açmış, düşük konsantrasyonlarda ise embriyo ve larvalarda tamamlanmayan gastrulasyon, kuyruk kıvrılması, zayıf renklenme, baş anormalliği, perikardiyal ödem, omurga sütun defekti ve skolyoz gibi subletal etkiler gözlenmiştir. Üstelik motor yağı konsantrasyonunun artışıyla beraber malformasyonda da bir artışın olduğu belirlenmiştir. Aynı şekilde koryondan çıkışlar gecikmiş ve larva boyu da kısa kalmıştır. Sonuç olarak yapılan çalışmada 10W-40 motor yağının sulu fazının zebra balığı embriyolarının normal gelişimini olumsuz etkilediği bulunmuştur.

10W-40 Motor Yağının Zebra Balığı Embriyolarının Erken Gelişim Evreleri Üzerine Toksisitesi

In this study, toxicity of water associated fraction of 10W-40 motor oil was investigated during the early life stages of zebrafish (Danio rerio). In 1.5-hour post fertilization (hpf), the fertilized zebrafish eggs were exposed to the oil fraction (1.25, 2.5, 5.0,10.0, 20.0 and 40.0 v/v) and were followed through 24, 48, and 72 hpf. The morphological endpoints of development toxicity of the oil in zebrafish embryos were observed under a stereomicroscope. Higher concentrations caused acute lethal effects, and lower concentrations caused sublethal effects, such as incomplete gastrulation, tail curvature, weak pigmentation, head malformation, pericardial edema, vertebra column defect and scoliosis. Moreover, with the increase of the oil concentration, the malformation rate increased. The hatching rate and body length were also decreased. Finally, it was found that the water associated fraction of 10W-40 motor oil affected the normal embryonic development of zebrafish.

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