MURAT ARSLAN, Selma ALAYBAŞI, ERkan ALTUN, Sinem GÜLEN, Necdet SİRKECİOĞLU, ALİ ATASEVER, Halil İbrahim HALİLOĞLU, Mevlüt ARAS

Changes in Lipids, Fatty Acids, Lipid Peroxidation and Antioxidant Defence System During the Early Development of Wild Brown Trout (Salmo trutta)

Reaktif oksijen türlerinin (ROS) üretimi oksijenli solunumun bir sonucu olup, balıkların erken gelişim dönemlerindeki hızlı büyüme ve buna bağlı yüksek düzeyde oksijen tüketiminden dolayı kritik duruma gelmektedir. Mevcut çalışma, ilk kez doğal sularda yaşayan kahverengi alabalığın (Salmo trutta) erken gelişim dönemi boyunca lipit, yağ asitleri, lipit peroksidayonu ve antioksidan savunma sitemindeki değişimleri incelemektedir. Toplam lipitler yumurtadan (%9,3) serbest yüzmeye geçen yavrulara (%4,3) kadarki dönemde önemli derecede düşüş göstermiştir. Dokozahekzaenoik asit (DHA; 22:6n3) embriyonik gelişim boyunca tercihi olarak vücutta tutulan yağ asidi olmuştur. Enzimatik olmayan antioksidanlar (vitamin, E, C ve B1) yumurtada bol miktarda bulunurken embriyonik gelişim boyunca önemli derecede düşüş gösterirken, katalaz (CAT), süperoksit dismütaz (SOD) ve glutatyon S-transferaz (GST) gibi antioksidan enzimlerin aktivitesi artış göstermiştir. Lipit peroksidasyon ürünü olan malondialdehit (MDA) en düşük seviyede yumurta ve gözlenmiş embriyoda bulunurken, en yüksek seviyede keseli yavru safhasında bulunmuştur. Çalışmamızın sonucuna göre enzimatik olmayan antioksidanlar embriyonik gelişim boyunca reaktif oksijen türlerinin zararlı etkilerini giderirken, yumurtadan çıkıştan sonra bu görevi antioksidan enzimler devralmaktadır.

Yabani Kahverengi Alabalığın (Salmo trutta) Erken Gelişim Döneminde Lipit, Yağ Asidi, Lipit Peroksidasyonu ve Antioksidan Savunma Sistemindeki Değişimler

Generation of radical oxygen species (ROS) is a natural consequence of aerobic metabolism and it becomes more critical during the early development of fish due to the rapid tissue growth resulting in high oxygen consumption. The present study was conducted as the first to evaluate the changes in lipids, fatty acids, lipid peroxidation and antioxidant defense system during the early development of wild brown trout (Salmo trutta). Total lipids dramatically decreased from 9.3% (egg) to 4.3% (swim-up). Docosahexaenoic acid (DHA; 22:6n-3) was the predominant fatty acid in all cases and was preferentially conserved during the early development. Non-enzymatic antioxidant scavengers (vitamin E, C and B1) were abundant in egg and decreased dramatically after hatching while the activities of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) increased at the same circumstance. The lowest malondialdehyde (MDA), lipid peroxidation product, was in unfertilized eggs and eyed-stage embryo while the highest level was observed in yolk-sac larvae. Our overall results suggest that high level of non-enzymatic free radical scavengers detoxify ROS during the embryonic development and elevated antioxidant enzymes take this duty over after hatch, protecting embryo and fry from oxidative stress.

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