Effects of Tricaine as an Anaesthetics on Goldfish, Carassius Auratus (Linnaeus 1758) at Different Salinities and Concentrations

Bu çalışmada, tricaine metansülfonat (MS-222)'ın beş farklı tuzluluk ve konsantrasyonda bir anestezik olarak japon balıkları üzerine etkileri saptanmıştır. Japon balıkları (230±25 mg ve 24,07±5,59 mm) 150, 200, 300, 400 ve 500 mg l-1 MS222 konsantrasyonlarına ve 0, 8, 12, 14 ve 16 ppt tuzluluk değerlerine maruz bırakılmıştır. Yemeklik balıklarda anestezik kullanımı üzerine bir çok literatür olmasına rağmen, süs balıkları yetiştiriciliği üzerine fazla bir bilgi bulunmadığı görülmektedir. Her bir tuzluluk için beş tricaine metansülfonate konsantrasyonu japon balıklarını anestezi yapmakta kullanıldı ve anestezi ve iyileşme süreleri kayıt edildi. Anestezi için gerekli zaman, konsantrasyon yoğunluğuna ve tuzluluk değerelerine göre değişmiştir. Her bir konsantrasyon maruziyetinde, balıklar derin anesteziye girdiler (anestezi süresi aralığı 0,13-8,90 dak). Balık ölümlerini ve stresi azalmak için ideal MS-222 konsantrasyonun 12 ppt tuzlulukta 200 mg l-1 olduğu tavsiye edilmiştir.

Farklı Tuzluluk ve Konsantrasyonlarda Tricaine'nin Bir Anestezik Olarak Japon Balıkları, Carassius auratus (Linnaeus 1758) Üzerine Etkileri

The present experiment was designed to determine the effects of tricaine methanesulfonate (MS-222) as an anaesthetic on goldfish Carassius auratus at five different salinities and MS-222 concentrations. Goldfish (230±25 mg and 24.07±5.59 mm) were exposed to 150, 200, 300, 400, and 500 mg l-1 MS-222 concentrations at 0, 8, 12, 14, and 16 ppt of salinities. Even though, a lot of literature exist about the anesthetic usage on foodfish species, not much information seems to be available on ornamental fish aquaculture. Five tricaine methanesulfonate concentrations for each salinity were used to anesthetize goldfish and recorded their induction and recovery times. The necessary time to make anesthesia on fish relied on concentration intensity and salinity. When exposed to any of the concentrations, fish achieved a deep state of anesthesia (range of induction time 0.13 and 8.90 min). It is recommended that ideal concentration of MS-222 was 200 mg l-1 at 12 ppt to reduce fish mortality and stress.

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Bystriansky, J.S., LeBlanc, P.J. and Ballantyne, J.S. 2006. Anaestetization of arctic charr Salvelinus alpinus (L.) with tricaine methanesulfonate or 2- phenoxyethanol for immediate blood sampling. Journal of Fish Biology, 69:613-621
Carter, K.M., Woodley, C.M. and Brown, R.S. 2011. A review of tricaine methanesulfonate for anesthesia of fish. Reviews in Fish Biology and Fisheries, 21:51-59. Doi: 10.1007/s11160-010-9188-0
Congleton, J.L. 2006. Stability of some commonly measured blood chemistry variables in juvenile salmonids exposed to a lethal dose of the anaesthetic MS-222. Aquaculture Research, 37:1146-1149. Doi: 10.1111/j1365-2109.2006.01528.x
Coyle, S.D., Durborow, R.M. and Tidwell, J.H. 2004. Anesthetics in aquaculture. Southern Regional Aquaculture Center, SRAC Publicaton No: 3900.
Gomulka, P., Wlasow, T., Velisek, J., Svobodova, Z. and Chmielinska, E. 2008. Effects of eugenol and MS-222 anaesthesia on Siberian sturgeon Acipenser baerii Brandt. Acta Veterinaria Brno, 77:447-453. Doi: 10.2754/avb200877030447
Hseu, J., Yeh, S., Chu, Y. and Ting, Y. 1998. Comparison of efficacy of five anesthetics in Goldlined sea bream, Sparus sarba. Acta Zoologica Taiwanica, 9:(1) 35-41.
Holloway, A.C., Keene, J., Noakes, D.G. and Moccia, R.D. 2004. Effects of clove oil and MS-222 on blood hormones profiles in rainbow trout Onchorhynchus mykiss, Walbaum. Aquaculture Research, 35:1025- 1030. Dio: 101111/j.1365-2109.2004.01108.x
Keene, J.L., Noakes, D.L.G., Moccia, R.D. and Soto, C.G. 1998. The efficacy of clove oil as an anaesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, 29:89-101.
King, W., Hooper, V.B., Hillsgrove, S., Benton, C. and Berlinsky, D.I. 2005. The use of clove oil, metomidate, tricaine methanesulphonate and 2- phenoxyethanol for inducing anaesthesia and their effect on the cortisol stress response in black sea bas (Centropristis striata L.). Aquaculture Research, 36:1442-1449. Doi: 10.1111/j1365-2109.2005.01365x
Küçük, S. 2010. Efficacy of tricaine on Poecilia latipinna at different temperatures and Concentrations. African Journal of Biotechnology, 9(5):755-759.
Lemm, C.A. 1993. Evaluation of five anaesthetics on striped bass. Resource publication. U. S. Fisheries and Wildlife Service. p 196.
Lepic, P., Stara, A., Turek, J., Kozak, P. and Velisek, J. 2014. The effects of four anaesthetics on haematological and blood biochemical profiles in vimba bream, Vimba vimba. Veterinarni Medicina, 59:2 81-87.
Massee, K.C., Rust, M.B., Hardy, R.W. and Stickney, R.R. 1995. The effectiveness of tricaine, quinaldine sulfate and metomidate as anesthetics for larval fish. Aquaculture, 134:351-359.
Malmstrom, T., Salte, R., Gjoen, H.M. and Linseth, A. 1993. A practical evalution of metomidate and MS- 222 as anaesthetics for Atlantic halibut (Hippoglossus hippoglossus L.). Aquaculture, 113:331-338.
Mattson, N.S. and Riple, T.H. 1989. Metomidate, a better anesthetic for cod (Gadus morhua) in comparison with benzocaine, MS_222, chlorobutanol and phenoxyethanol. Aquaculture, 83:89-94.
Mazik, P.M. and Simco, B.A. 2014. The effects of size, water hardness, salt levels and MS-222 on the survival and stress response in striped bass (Morone saxatilis). http://fishphysiology.org/wp-content/uploads/2014/
Mazik, P.M., Simco, B.A. and Parker, N.C. 1991. Influence of water hardness and salts on survival and physiological characteristics of striped bass during and after transport. Transactions of the American Fisheries Society, 120 (1):121-126.
Mercy, T.V.A., Malika, V. and Sajan, S. 2013. Use of tricaine methanesulfonate (MS-222) to induce anaesthesia in Puntius denisonii (Day, 1865) (Teleostei: Cypriniformes:Cyprinidae), a threatened barb of the Western Ghats, India. Journal of Threatened Taxa, 5:(9) 4414-4419. Doi: http://dv.doi.org/10.11609/joTTo3294.4414-9
Munday, P.L. and Wilson, S.K. 1997. Comparative efficacy of clove oil and other chemicals in anaesthetization of Pomacentrus amboinensis, a coral reeffish. Journal of Fish Biology, 51:931-938.
Mylonas, C.C., Cardinaletti, G., Sigelaki, I. and PolzonettiMagni, A. 2005. Comperative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gillhead sea bream (Sparus auratus) at different temperature. Aquaculture, 246:467-481. Doi: 10.1016/j.aquaculture.2005.02.046.
Pawar, H.B., Sanaye, S.V., Sreepada, R.A., Harish, V., Suryavanshi, U., Ansari, T. and Ansari, Z.A. 2011. Comparison of efficacy of four anaesthetic agents in the Yellow Seahorse, Hippocampus kuda (Bleeker, 1852). Aquaculture, 311:155-161. Doi: 10.1016/j.aquaculture.2010.12007
Popovic, N.T., Strunjak-Perovic, I., Coz-Rakovac, R., Barisic, J., Jadan, M., Persin Berakovic, A. and Sauerborn Klobucar, R. 2012. Review: Trivaine methane-sulfonate (MS-222) application in fish anaesthesia. Journal of Applied Ichthyology, 28:553- 564.
Pramod, P.K., Ramachandran, A., Sajeevan, T.P., Thampy, S. and Pai, S.S. 2010. Comparative efficacy of MS- 222 and benzocaine as anaesthetics under simulated transport conditions of a tropical ornamental fish Punctius filamentosus (Valenciennes). Aquaculture Research, 41:309-314. Doi: 10.1111/j.1365- 2109.2009.02333.x
Sneddon, L. 2012. Clinical anesthesia and analgesia in fish. Journal of Exotic Pet Medicine, 21(1):32-43.
Small, B. 2003. Anaesthetic efficacy of metomidate and comparison of plasma cortisol responses to tricaine methanesulfonate, quinaldine and clove oil anaesthetized channel catfish Ictalurus punctatus. Aquaculture, 218:177-185.
Sylvester, J.R. 1975. Factors influencing the efficacy of MS-222 to striped mullet (Mugil cephalus). Aquaculture, 6:163-169.
Tomasso, J.R., Davis, K.B. and Parker, N.C. 1980. Plasma corticosteroid and electrolyte dynamics of hybrid striped bass (white bass x striped bass) during netting and hauling. Proceedings of the World Mariculture Society, 11: 303-310.
Tort, L., Puigcerver, M., Crespo, S. and Padros, F. 2002. Cortisol and haematological response in sea bream and trout subjected to the anaesthetics clove oil and 2- phenoxyethanol. Aquaculture Research, 33:907-910.
Tsantilas, H., Galatos, A.D., Athanassopoulou, F., Prassinos, N.N. and Kousoulaki, K. 2006. Efficacy of 2-phenoxyethanol as an anaesthetic for two size classes of white sea bream, Diplodus Sargus L., and sharp snout sea bream, Diplodus puntazzo C. Aquaculture, 253:64-70.
Wagner, G.N., Singer, T.D. and McKinley, R.S. 2003. The ability of clove oil and MS-222 to minimize handling stress in rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture Research, 34:1139-1146.
Walsh, C.T. and Pease, B.C. 2002. The use of clove oil as an anaesthetic for the longfinned eel, Anguilla reinhardtii (Steindachner). Aquaculture Research, 33:627-635.
Waterstrat, P.R. 1999. Induction and recovery from anaesthesia in channel catfish Ictalurus punctatus fingerlings exposed to clove oil. Journal of World Aquaculture Society, 30:250-255.
Weber, R.A., Peleteiro, J.B., Gracia-Martin, L.O. and Aldegunde, M. 2009. The efficacy of 2- phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agent in the Senegalese Sole (Solea senegalensis Kaup, 1858). Aquaculture, 288:147-150. Doi: 10.1016/jaquaculture.2008.11024
Weyl, O., Kaiser, H. and Hecht, T. 1996. On the efficacy and mode of action of 2-phenoxyethanol as an anaesthetic for goldfish, Carassius auratus (L.), at different temperatures and concentrations. Aquaculture Research, 27:757-764.