Protective Action of an Anti-oxidant (vitamin-C) against bisphenol-toxicity in cirrhinus mrigala (ham.)

Denemede 55±10 g ağırlığında 45 adet Cirrhinus mrigala balığı her biri 15 adet olmak üzere 3 gruba ayrılmıştır. 15, 30 ve 60 gün boyunca 1. gruba yalnızca balık yemi verilmiş ve kontrol grubu olarak belirlenmiş, ikinci grup bisfenol-A’ya (BPA) (2 mg/L) ve 3. grup ise C-vitaminine (50 mg/L) ve BPA’ya (2 mg/L) maruz bırakılmıştır. Balıkların karaciğerinde ve böbreklerinde histopatolojik değişimler gözlemlenmiştir. Onbeş gün süreyle Bisfenol-A’ya maruz bırakılan balıklarda hepatik ve renal hücrelere bağlı dejeneratif değişiklikler meydana gelmiştir. Fakat bu değişiklikler daha sonraki kısımda, yani deneylerin 30 ve 60. günlerinde daha belirgin ve şiddetli hale gelmiştir. Kontrol grubuna kıyasla 30 ve 60 gün boyunca BPA’ya maruz bırakılan hayvanlarda, ürünifer tübüllerde atrofik değişiklikler ve hipertrofi tanısı konan hepatik nükleuslar, hepatositlerde ve interrenal hücrelerde nekroz izlenmiştir. BPA’ya maruz kalanlara kıyasla 15, 30 ve 60 gün sonra, C vitaminiyle birlikte BPA’ya maruz kalan hayvanların hepatik hücrelerinde, interrenal hücrelerinde ve ürünifer tübülllerinde iyileşme görülmüştür. C vitamininin yani antioksidanın, Cirrhinus mrigala’da bisfenol-A toksisitesine karşı panzehir rolü veya koruyucu tedbir dozu özelliği olduğu yapılan gözlemlerle işaret edilmiştir.

Anahtar Kelimeler:

hipertrofi, toksikoloji, histopatoloji, diyetler, Cirrhinus mrigala, antioksidanlar, askorbik asit, atropi, yem zenginleştiriciler, balık beslenmesi, doku ölümü, toksisite

Cirrhinus mrigala’da (ham.) bisfenol-A toksisitesine karşın bir antioksidanın (C vitamini) koruyucu etkisi

45 fishes Cirrhinus mrigala weighing 55±10 g were divided into 3 groups of fifteen each. Group $1^ {st}$ received fish diet only and served as control, while group $2^ {nd}$ exposed with bisphenol-A (BPA) (2 mg/L) and group $3^ {rd}$ exposed with vitamin-C (50 mg/L) and BPA (2 mg/L) for 15, 30 and 60 days and histopathological studies were observed in their liver and kidney. Bisphenol-A showed degenerative changes in hepatic and renal cells after 15 days exposures. However, these changes were more prominent and severe in later part i.e. 30 and 60 of the experiments. The animals exposed with BPA for 30 and 60 days showed atrophied changes in uriniferous tubules and hypertrophied hepatic nuclei, necrosis in hepatocytes and interrenal cells as compared to control group. The animals exposed with vitamin-C along with BPA showed recovery in their hepatic cells, interrenal cells, and uriniferous tubules after 15, 30 and 60 days as compared to BPA groups. These observations denotes that vitamin-C i.e. antioxidant has some antidote role or protective action against bisphenol-A toxicity in Cirrhinus mrigala.

Keywords:

hypertrophy, toxicology, histopathology, diets, Cirrhinus mrigala, antioxidants, ascorbic acid, atrophy, feed supplements, fish feeding, necrosis, toxicity,

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