Prevalence of Marteilia spp. in Thirteen Shellfish Species Collected from China Coast

Marteilia spp. is listed as a notifiable parasite by the Office International des Epizooties (OIE), and cause physiologicaldisorders and eventually death of the shellfish. Little is known about the prevalence of Marteilia spp. in shellfish of China.Therefore we investigated the prevalence of Marteilia spp. in 13 species of shellfish collected in the coastal areas of Chinabetween 2006 to 2012. A total of 11,581 individual shellfish comprising 13 different species was collected from seven bayareas in China. The shellfish was tested for the prevalence of Marteilia spp. following a PCR protocol recommended by OIE.Marteilia spp. was found in the shellfish samples （Crassostrea rivularis, Crassostrea gigas and Crassostrea angulata）,Meretrix meretrix, Chlamys nobilis, Ruditapes philippinarum, Perna viridis, Tegillarca granosa, and Sinonovacula constricta.The detetion rates of Marteilia spp. by PCR method ranged from 0% to 6.67%, with R. philippinarum (6.67%) showing thehighest detection rate. Marteilia spp. sequence of the individual positive shellfish had a high sequence homology (97.4% to99.6%) with the sequences of other Marteilia type “M”. Mixed positive of Marteilia spp. with other protozoan species(Haplosporidium nelsoni and Perkinsus spp.) was a common phenomenon (26.28%).

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