Rasoul SHAHROOZ, Naser AGH, Nasim JAFARI, Ali KALANTARI, Reza JALILI, Ali KARIMI

Effects of Fish Oil Replacement with Vegetable Oils in Rainbow Trout (Oncorhynchus mykiss) Fingerlings Diet on Growth Performance and Foregut Histology

The present study was performed to elucidate the effects of total replacement of fish oil with vegetable oils on growthperformance and foregut histological indices of the rainbow trout. Fishes with average initial weight of 15.10±0.20 g weretransferred into 15 (300 L) tanks (50 fishes/tank) and reared for 60 consecutive days using different experimental diets. Kilkafish oil (FO) was fully replaced with different vegetable oil sources including canola oil (CO), safflower oil (SO), linseed oil(LO) and a mixture (CSLO) of CO (40%), SO (30%) and LO (30%) as dietary lipid sources. The samples were fixed at 10%neutral-buffered formalin and paraffin sections were prepared and stained with hematoxylin and eosin and periodic acidSchiff methods. Growth performances in CO and CSLO groups were better than other groups. Moreover, in these groups, thenumber of goblet cells was decreased; however thickness and height of intestinal folds and thickness of tunica mucosa andtunica sub-mucosa were increased in comparison to FO group. Overall, performance and histological findings did not showany negative effect due to canola and mixed vegetable oils feeding compared to FO group.

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