MEVLÜT AKTAŞ, Oğuz CİĞER, ERCÜMENT GENÇ, M. Ayçe GENÇ, Nuran ÇAVDAR

Effects of mannan oligosaccharide and serotonin on molting, growth, body composition and hepatopancreas histology of white Leg Shrimp Litopenaeus vannamei (Boone 1931)

Mannan oligosakkarit, serotonin ve her ikisinin birlikte kullanımının Pasifik karidesi, Litopenaeus vannameinin kabuk değişimi, büyüme, yaşama oranı, et kompozisyonu ve hepatopankteas histolojisi üzerine etkileri 75 günlük bir çalışma sürecinde araştırılmıştır. 3 g kg-1 MOS, 20 mg kg-1 düzeyinde serotonin ve her ikisinin birlikte aynı düzeyde kullanımının etkileri kontrol grubu ile karşılaştırılarak test edilmiştir. 1.35±0.04 g başlangıç ağırlığına sahip karidesler 10,71(kontrol), 11,05 (serotonin) ve 12,26 gram (MOS) ağırlığa ulaşmışlardır. MOS destekli yemlerle beslenen grupta yaşama oranı en yüksek olmasına ragmen (%86) gruplar arası istatistiki fark bulunamamıştır (P>0,05). 3 g kg-1 düzeyinde MOS katkılı yemlerle beslenen karideslerin yaşama oranı, kabuk değiştirme sıklığı, büyüme oranı ve FCRı kontrol grubuna göre önemli ölçüde etkilemiştir. 20 mg/kg düzeyinde yemlere eklenen serotonin aynı parametreleri pozitif yönde etkilemesine rağmen kabuk değiştirme sıklığı üzerine olumsuz etkide bulunmuştur (P

Mannan oligosakkarit ve seretonin in beyaz bacaklı karides litopenaeus vannamei (boone) nin kabuk değişimi, büyüme, vücut kompozisyonu ve hepatopankreas histolojisi üzerine etkileri

The effects of dietary mannan oligosaccharide (MOS), serotonin (5-HT) and the combination of both on molting, growth, survival, body composition, and hepatopancreas histology of the Pacific white shrimp Litopenaeus vannamei were investigated for 75 days. Dietary inclusion level of MOS at 3 g kg -1, serotonin at 20 mg kg-1, and both were tested in triplicate groups against a control diet lacking MOS or serotonin. The shrimps having an average initial weight of 1.35±0.04 g attained to final weights an average of 10.71 control, 11.05 serotonin and 12.26 MOS respectively. Although survival rate was high (86%) in the shrimp fed MOS-supplemented diet, no statistical difference was found among the groups (P>0.05). The results indicate that inclusion of 3 g kg-1 MOS into diet enhance shrimp survival, moulting rate, growth and FCR compared to the control. Serotonin inclusion at 20 mg/kg also improved the same parameters, but suppressed moulting rate in comparison to the other groups (P<0.05). Hepatopancreatic tissues were not affected by dietary treatments. Body composition (protein, lipid, ash and dry matter) of the shrimps were also similar in all the dietary groups. In conclusion, the current results have demonstrated that MOS at 3.0 g kg-1 inclusion level could be used as growth promoter in L. vannamei diets.

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Aktas, M. and Kumlu, M. 2005. Gonadal maturation and spawning in Penaeus semisulcatus de Hann, 1844 by hormone injection. Turk Journal of Zoolgy, 29(3): 193-199.
Al-Mohanna, S.Y. and Nott, J.A. 1987. R-cells and the digestive cycle in Penaeus semisulcatus (Crustacea: Decapoda). Marine Biology, 95: 129-137.
AOAC, 1995. Official methods of analysis of AOAC International. 2 vols. 16th Edition. Arlington, VA, USA, Association of Analytical Communities.
Arechiga, H., Flores, J. and Garcia, U. 1985. Biosynthesis and release of the crustacean neurodepressing hormone. In: B. Lofts, W.N. Holmes (Eds.), Current Trends in Comparative Endocrinology: proceedings of the Ninth International Symposium on Comparative Endocrinology 7-11 December 1981 Hong Kong University, Hong Kong: 787-791.
Bligh, E.G. and Dyer, W.J. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol, 37: 911-917.
Cabello, F.C. 2006. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ. Microbial, 8(7): 1137-1144. doi:10.1111/j.1462-2920.2006.01054.x
Chotikachinda, R., Lapjatupon, W., Chaisilapasung, S., Sangsue, D. and Tantikitti, C. 2008. Effect of inactive yeast cell wall on growth performance, survival rate and immune parameters in Pacific White Shrimp (Litopenaeus vannamei). Songklanakarin J. Sci. Technol., 30(6): 687-692.
Dall, W. Hill, B.J. Rothlisberg, P.C. and Sharples, D.J. 1990. The Biology of the Penaeidae. In: J.H.S. Blaxter, A.J. Soutward (Eds), Advances in Marine Biology, Vol 27. Academic Press, London, San Diego, New York, Boston, Sydney, Tokyo, Toronto. 489 pp.
Dimitroglou, A., Davies, S. and Sweetman, J. 2008. The effect of dietary mannan oligosaccharides on the intestinal histology of rainbow trout (Oncorhynchus mykiss). Comp. Biochem. Physiol., 150(3): 62-63. doi:10.1016/j.cbpa.2008.04.068
Dimitroglou, A., Merrifield, D.L., Moate, R., Davies, S.J., Spring, P., Sweetman, J. and Bradley, G. 2009. Dietary mannan oligosaccharide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout, Oncorhynchus mykiss (Walbaum). J. Anim. Sci. 87(10): 3226-3234. doi: 10.2527/jas.2008-1428
Gatlin, D.M. 2002. Nutrition and Fish health. In: J.E. Halver, R.W. Hardy (Eds.), Fish nutrition, Academic Press, San Dieogo, CA USA: 671-702.
Gatlin, D.M., Li, P., Wang, X., Burr, G.S., Castille, F., and Lawrence, A.L. 2006. Potential application of prebiotics in aquaculture. In: Avances en Nutrición Acuicola. (Eds. L. Elizabeth Cruz Suárez, Denis Ricque Marie, Mireya Tapia Salazar, Martha G. Nieto López, David A.Villarreal, Ana C. Puello Cruz y Armando Garcia Ortega). VIII. Simposium Internacional de Nutricion Acuicola. 15-17 Noviembre. Universidad Autónoma de Nuevo León, Monterry, Nuevo León Mexico. ISBN 970-694-333-5.
Gatlin, D.M. and Burr, G. 2009. Effects of the prebiotics GroBiotic®-A and Inulin on the intestinal microbiota of red drum, Sciaenops ocellatus, J World Aquac Soc 40: 440-449. doi: 10.1111/j.1749-7345.2009.00271.x
Genc, M.A., Aktaş, M., Genc, E. and Yılmaz, E. 2007. Effects of dietary mannan oligosaccharide on growth, body composition and hepatopancreas histology of Penaeus semisulcatus (de Haan 1844). Aquaculture Nutrition, 13: 156-161. doi: 10.1111/j.1365- 2095.2007.00469.x
Gibson, G.R. and Roberfroid, M.B. 1995. Dietary modulation of the human colonic microbiota introducing the concept of prebiotics. J. Nutr., 125: 1401-1412.
Hillman, K. 2001. Bacteriological aspects of the use of antibiotics and their alternatives in the feed of nonruminant animals. In: P.C. Garnsworthy, J. Wiseman, (Eds.), Recent Advances in Animal Nutrition, Nottingham University Press, UK 107-134.
Keller, R., Jaros, P.P. and Kegel, G. 1985. Crustacean hyperglycemic neuropeptides. Am. Zool., 25: 207-221
Kulkarni, G., Nagabhushanam, K.R., Amaldoss, G., Jaiswal, R.G. and Fingerman, M. 1992. In vivo stimulation of ovarian development in the red swamp crayfish, Procambarus clarkii (Girard), by 5 - hydroxytryptamine. Invertebr. Reprod. Dev., 21: 231- 240.
Kumlu, M., Türkmen, S., Kumlu, M. and Eroldoğan, O.T. 2011. Off-season maturation and spawning of the Pacific white shrimp Litopenaeus vannamei in sub- tropical conditions. Turkish Journal of Fisheries and Aquatic Sciences, 11: 15-23. doi:10.4194/trjfas.2011.0103
Li, P. and Gatlin, D.M. 2004. Dietary brewers yeast and the prebiotic GrobioticTM AE influence growth performance, immune response and resistance of hybrid striped bass (Morone chrysops×M. saxatilis) to Streptococcus iniae infection. Aquaculture, 231: 445- 456. doi:10.1016/j.aquaculture.2003.08.021
Li, P., Burr, G.S., Gatlin, D.M., Hume, M.E., Patnaik, S., Castille, F.L. and Lawrence, A.L. 2007. Dietary supplementation of short-chain fructooligosaccharides influences gastrointestinal microbiota composition and immunity characteristics of pacific white shrimp, Litopenaeus vannamei, cultured in a recirculating system. J. Nutr., 137: 2763-2768. doi: 0022-3166/07
Mahious, A.S., Gatesoupe, F.J., Hervi, M., Metailler, R. and Ollevier, F. 2006. Effect of dietary inulin and oligosaccharides as prebiotics for weaning turbot, Psetta maxima (Linnaeus, 1758). Aquacult. Int., 14: 219-229. doi 10.1007/s10499-005-9003-4
Mattson, M.P. and Spaziani, E. 1985. Characterization of molt inhibiting hormone (MIH) action on crustacean Y-organ segments and dispersed cells in culture, and a bioassay for MIH activity. J. Exp. Zool., 236: 93-102.
Nontawith, A. 2008. Effects of mannan-oligosaccharide as feed supplement on growth, survival and disease resistance of Nile Tilapia (Oreochromis niloticus Linnaeus) fry. 5th World Fisher. Congress (WFC 2008), Pacifico Yokohama 20-24 Oct. Yokohama (Japan)
Parsons, T.R., Maita, Y. and Lalli, C.M. 1984. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, Oxford, 173 pp.
Rao, K.R. and Fingerman, M. 1975. Color changes induced by certain indole alkaloids in the fiddler crab, UCA. Comp. Biochem. Phys., 51: 59-62.
Refstie, S., Landsverk, T., Bakke-McKellep, A.M., Ringø, E., Sundby, A., Shearer, K.D. and Krogdahl, A. 2006. Digestive capacity, intestinal morphology, and micro flora of 1-year and 2-year old Atlantic cod (Gadus morhua) fed standard or bioprocessed soybean meal. Aquaculture, 261: 269-284. doi:10.1016/j.aquaculture.2006.07.011
Roberts, R.J. and Smail, D.A. 2001. Laboratory methods. In: R.J. Roberts (Ed.), Fish pathology, 3rd Edition. W.B. Saunders, London: 380-412.
Sainath, S.B. and Reddy, P.S. 2011. Effects of selected biogenic amines on reproduction in the fresh water edible crab, Ozitelphusa senex senex. Aquaculture 313: 144-148. doi:10.1016/j.aquaculture.2011.01.010
Sang, H.M. and Fotedar, R. 2010. Effects of mannan oligosaccharides dietary supplementation on performances of the tropical spiny lobsters juvenile (Panulirus ornatus, Fabricius 1798). Fish Shellfish Immunol., 28: 483-489. doi: 10.1016/j.fsi.2009.12.011
Sang, H.M., Ky, L.T. and Fotedar, R. 2009. Dietary supplementation of mannan oligosaccharide improves the immune responses and survival of marron, Cherax tenuimanus (Smith, 1912) when challenged with different stressors. Fish Shellfish Immunol., 2: 341- 348. doi:10.1016/j.fsi.2009.06.003
Salze, G., McLean, E., Schwarz, M.H. and Craig, S.R. 2008. Dietary mannan oligosaccharide enhances salinity tolerance and gut development of larval cobia. Aquaculture, 274:148-152. doi:10.1016/j.aquaculture.2007.11.008
Sarojoni, R., Nagabhushanam, R. and Fingerman, M. 1994. A possible neurotransmitter-neuroendocrine mechanism in naphthalene-induced atresia of the ovary of the red swamp crayfish, Procambarus clarkii. Comp. Biochem. Physiol., 108: 33-38.
Staykov, Y., Spring, P., Denev, E.S. and Sweetman, E.J. 2007. Effect of a mannan oligosaccharide on the growth performance and immune status of rainbow trout (Oncorhynchus mykiss). Aquacult. Int., 15: 153- 161. doi 10.1007/s10499-007-9096-z
Takashima, F. and Hibiya, T. 1995. An atlas of fish histology normal and pathological features, 2nd Edition. Kodansha Ltd., Tokyo, 195 pp.
Torrecillas, S., Makol, A., Caballero, M.J., Montero, D., Robaina, L., Real, F., Sweetman, J., Tort, L. and Izquierdo, M.S. 2007. Immune stimulation and improved infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides. Fish Shellfish Immun., 23: 969-981. doi:10.1016/j.fsi.2007.03.007
Vogt, G., Storch, V., Quinitio, E.T. and Pascual, F.P. 1985. Midgut gland as monitor organ for the nutritional value of diets in Penaeus monodon (Decapoda). Aquaculture, 48: 1-12.
Vaca, A. and Alfaro, J. 2000. Ovarian maturation and spawning in the white shrimp, Penaeus vannamei, by serotonin injection. Aquaculture, 182: 373-385. doi:10.1016/S0044-8486(99)00267-7
Villalon, J.R. 1991 Practical manual for semi-intensive commercial production of marine shrimp. TAMU-SG- 91501. TAMU Sea Grant college program, college station, Texas.
Yilmaz, E., Genc, M.A. and Genc, E. 2007. Effects of dietary mannan oligosaccharides on growth, body composition, and intestine and liver histology of rainbow trout, Oncorhynchus mykiss, Isr. J. Aquacult- Bamid, 59: 182-188.