Effect of synthetic luteinizing hormone - releasing hormone (LHRH-A2) plus pimozide and chlorpromazine on ovarian development and Levels of gonad steroid hormones in female kutum rutilus frisii kutum

To assess changes of gonad development and sex steroids hormones of R. frisii kutum, broodfish were captured during spawning season 2010 (March-April) and induced intramuscularly with following hormone treatments: single injections of 1 μg kg-1 b.w. LHRH-A2, combination of LHRH-A2 with two dopamine antagonists as follows: 1 μg kg-1 b.w. LHRH-A2 + 5 mg kg-1 b.w. PIM, 1 μg kg-1 b.w. LHRH-A2 + 2.5 mg kg-1 b.w. chlorpromazine, 1 μg kg-1 b.w. LHRH-A2 + 5 mg kg-1 b.w. PIM + 2.5 mg kg-1 b.w. chlorpromazine, single injections of 5 mg kg-1 b.w. PIM and 2.5 mg kg-1 b.w. chlorpromazine, carp pitatury extract (CPE), saline solution (NaCl 0.7%) and control group (without injection) were used, respectively. The number of ovulated fish was different in each treatment. Ovaries were more progressed in ovulated rather than non-ovulated fish. Also, sex hormones such as testosterone (T) and estradiol (E2) were changed in different hormonal treatments. In ovulated fish, histological analysis revealed six stages in ovaries, while ovaries in non-ovulated ones were developed only in fourth stage.

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