Effects of increasing dietary protein on growth parameters, feed utilization were studied in Parachanna obscura larvae with initial body weight 0.12±0.01 g. The experiment was conducted during 28 days in re-circulating system composed of 15 tanks containing 20 L of water each. 50 larvae were stocked per tank. Five semi purified isoenergetic experimental diets were formulated with different protein level (35, 45, 50, 55 and 60 g/100 g of diet). Each diet was tested in triplicate. Growth parameters and feed utilization were significantly influenced by dietary protein (P<0.05). Specific Growth Rate varied from 8.86 to 11.53%/d. Best Specific Growth Rate, Feed Efficiency and Productive Protein Value were obtained with diet containing 55% of protein. The highest body protein content was found with larvae fed by diet with 55% of protein (P<0.05). Feed Efficiency and Productive Protein Value of fish fed on 45, 50 and 60% dietary protein were not significantly different (P>0.05). Second degree polynomial regression and broken line models were used to analyze the relationships between dietary crude protein and SGR. Based on the results of this study, protein requirements of P. obscura larvae ranged from 45 and 55.5% of the diet.
___
Akiyama, T., Oohara, I. and Yamamoto, T. 1997. Comparison of essential amino acid requirements with A/E ratio among fish species. Fish. Sci., 63: 963970
Akpinar, Z., Sevgili, H., Ozgen, T., Demir, A. and Emre, Y. 2011. Dietary protein requirement of juvenile shi drum, Umbrina cirrosa (L). Aquacult. Nutr., doi:10.1111/j.1365-2109.2011.02845.x
AliyuPaiko, M., Hashim, R. and ShuChien, A.C. 2010. Influence of dietary lipid/protein ratio on survival, growth, body indices and digestive lipase activity in Snakehead (Channa striatus, Bloch 1793) fry reared in recirculating water system. Aquacult. Nutr., 16: 466474. doi: 10.1111/j.13652095.2009.00683.x
Arzel, J., Metailler, R., Kereguer, C., Le Delliou, H. and Guillaume, J. 1995. The protein requirement of brown trout (Salmo trutta) fry. Aquaculture, 130: 67-78.
Bligh, E.G. and Dyer, W.J. 1959. A rapid method for lipid extraction and purification. Can. J. Biochem. Physiol., 37: 911917.
Bonou, C.A. and Teugels, G.G. 1985. Révision systématique du genre Parachanna Teugels et Daget, 1984 (Pisces, Channidae). Rev. Hydrobio. Trop., 18(4): 267-280.
Brett, J.R. and Grove, T.D.D. 1979. Physiological energetic. In: W.S. Hoar, D.J. Randall, J.R. Brett (Eds.), Fish Physiology. Bioenergetics and Growth, vol. VIII. Academic Press, New York: 279352.
De Silva, S.S., Gunasekera, R.M. and Atapattu, D. 1989. The dietary protein requirements of young tilapia and an evaluation of the least cost dietary protein levels. Aquaculture, 80: 271-284.
Diana, J.S., Chang, W.Y.B., Ottey, D.R. and Chuapoehuk, W. 1985. Production systems for commonly cultured freshwater fishes of Southeast Asia. International Program Report, Great Lake and Marine Water Center, University of Michigan, Ann. Arbor., 7: 75- 79.
Diyaware, M.Y., Modu, B.M. and Yakubu, U.P. 2009. Effect of different dietary protein levels on growth performance and feed utilization of hybrid catfish (Heterobranchus bidorsalis x Clarias anguillaris) fry in north-east Nigeria. Afric. J. Biotech., 8(16): 3954- 3957.
El-Sayed, A.F.M. and Teshima, S.I. 1992. Protein and energy requirements of Nile tilapia, Oreochromis niloticus, fry. Aquaculture, 103: 55-63.
Fagbenro, O.A., Balagon, A.M. and Anyanwu, C.N. 1992. Optimum dietary protein level of Heterobranchus bidorsalis fed compounded diet. Nig. J. Appl. Fish. Hydrobiol., 1: 41-45.
Farhat, K.M.A and Khan, M.A. 2011. Growth, feed conversion and nutrient retention efficiency of African catfish, Clarias gariepinus (Burchell) fingerling fed diets with varying levels of protein. J. Appl. Aquac., 23(4): 304-316. doi: 10.1080/10454438.2011.626370.
Fiogbé, E.D. and Kestemont, P. 1995. An assessment of the protein and amino acid requirements in goldfish (Carassius auratus) larvae. J. Appl. Ichthyol., 11: 282 289.
Fiogbé, E.D. 1996. Contribution à létude des besoins nutritionnels chez les larves et juvéniles de la perche fluviatile (Perca fluviatilis L.). Thèse de doctorat, FUNDP, Namur, 288 pp.
Gras, R. 1961 Liste des poissons du Bas-Dahomey faisant partie de la collection du laboratoire dhydrobiologie du service des eaux, forêts et chasses du Dahomey. Bull .I.F.A.N. 23 (2): 572 -586.
Günther, A. 1861. Catalogue of the Acanthopterygian fishes. The Trustees, London, 586 pp.
Hossain, M.A., Sultana, Z., Kibria, A.S.M. and Azimuddin, K.M. 2012. Optimum dietary protein requirement of a Thai Strain of Climbing Perch, Anabas testudineus (Bloch, 1792) fry. Turk. J. Fish. Aqua. Sci., 12: 217- 224. doi: 10.4194/1303-2712-v12_1_04.
Houlihan, D.F. 1991. Protein turnover in Ectotherms and its relationships to energetic. In: R. Gilles (Ed.), Advances in Comparative and Environmental Physiology, 7: 1- 43.
Jamabo, N.A. and Alfred-Ockiya, J.F. 2008. Effects of dietary protein levels on the growth performance of Heterobranchus bidorsalis (Geoffrey- Saint-Hillarie) fingerlings from Niger delta. Afr. J. Biotechnol., 7(14): 2483-2485.
Kaushik, S.J. and Médale, F. 1994. Energy requirements, utilization and dietary supply to salmonids. Aquaculture, 124: 81-97.
Kaushik, S.J., Doudet, T., Médale, F., Aguirre, P. and Blanc, D. 1995. Protein and energy needs for maintenance and growth of Nile tilapia (Oreochromis niloticus). J. Appl. Ichthyol., 11: 290 296.
Kerdchuen, N. 1992. Alimentation artificielle dun silure africain Heterobranchus longifilis (Teleostei: Clariidae): Incidence du mode dalimentation et première estimation des besoins nutritionnels. PhD thesis, Montpellier II University, 182 pp.
Kim, K.W., Wang, X.J. and Bai, S.C. 2002. Optimum dietary protein level for maximum growth of juvenile olive flounder, Paralichthys olivaceus (Temminck and Schlegel). Aquacult. Res., 33: 673-679.
Kim, K., Wang, X. and Bai, S.C. 2003. Reevaluation of the dietary protein requirement of Japanese flounder (Parlichthys olivaceus). J. World. Aquacult. Soc., 34(2): 133-139. doi: 10.1111/j.1749-7345.2003. tb00049.x
Kim, S.S. and Lee, K.J. 2009. Dietary protein requirement of juvenile tiger puffer, (Takifugu rubripes). Aquaculture, 287: 219-222.
Kpoguè, D. and Fiogbé, E. 2012. Optimum stocking density for Parachanna obscura larvae fed at its optimum ration. Int. J. Biol. Chem. Sci., 6 (3): 12931302. doi: http://dx.doi.org/10.4314/ijbcs.v6i3.32
Lovell, R.T. 1989. Nutrition and Feeding of Fish. Van Nostrand Reinhold, New York, USA, 260 pp.
Mohanty, S.S. and Samantary, K. 1996. Effects of varying levels of dietary protein on the growth performance and feed conversion efficiency of snakehead, Channa striata fry. Aquacult. Nutr., 2: 89-94.
Monentcham, S.E., Pouomogne, V. and Kestemont, P. 2010. Influence of dietary protein levels on growth performance and body composition of African fingerlings, Heterotis niloticus (Cuvier, 1829). Aquacult. Nutr., 16: 144-162. doi: 10.1111/j.1365- 2095.2008.00646.x.
NRC (National Research Council). 1993. Nutrient Requirements of Fish. National Academy Press, Washington, DC, 114 pp.
O Bryen, P.J. and Lee, C.S. 2007. Discussion summary: socioeconomic aspects of species and systems selection for sustainable aquaculture. In: P. Leung, C.S. Lee and P.J. O Bryen, (Eds.), Species and system selection for sustainable Aquaculture. Blackwell Publishing, Oxford: 477487.
Pillay, T.V.R. 1990. Aquaculture: Principles and Practices. Fishing News Books, Oxford, 575 pp.
Qin, J., Fast, A.W., De Anda, D. and Weidenbach, R.P. 1997. Growth and survival of larval snakehead (Channa striatus) fed different diets. Aquaculture, 148: 105-113.
Robbins, K.R., Norton, H.W. and Baker, D.H. 1979. Estimation of nutrient requirements from growth data. J. Nutr., 109: 1710-1714.
Samantary, K. and Mohanty, S.S. 1997. Interactions of dietary levels of protein and energy on fingerling snakehead, Channa striata. Aquaculture, 156: 241249.
Saville, D.J. 1990. Multiple comparison procedures: the practical solution. American Statistic, 44(2): 174180.
Schram, E., Van der Heul, J.W., Kamstra, A. and Verdergem, M.C.J. 2006. Stocking density dependent growth of dover (Solea solea). Aquaculture, 252: 239-247.
Shiau, S.Y. 2002. Tilapia, Oreochromis spp. Nutrient Requirements and Feeding of Finfish for Aquaculture. In: C.D.Webster and C.E. Lim (Eds.), Cabi Publishing, New York: 273-292.
Siddiqui, T.Q. and Khan, M.A. 2009. Effects of dietary protein levels on growth, feed utilization, protein retention efficiency and body composition of young Heteropneustes fossilis (Bloch). Fish. Physiol. Biochem., 35: 479488.
Tacon, A.G.J. 1987. The nutrition and feeding of farmed fish and shrimp. GCP/RAL/075/ITA, Field Document 2/E. Food and Agriculture Organization of the United Nations, Brazil.
Wee, K.L. and Tacon, A.G.J. 1982. A preliminary study on the dietary protein requirement of juvenile snakehead (Channa micropeltes). Bull. Jap. Soc. Sci. Fish., 48: 1463-1468. doi: 10.2331/suisan.48.1463
Wilson, R.P. and Halver, J.E. 1986. Protein and amino acid requirements of fishes. Annu. Rev. Nutr., 6: 225-244.
Yang, S.D., Lin, T.S., Liou, C.H. and Peng, H.K. 2003. Influence of dietary protein level on growth performance, carcass composition and liver lipid classes of juvenile Spinibarbus hollandi (Oshima). Aquacult. Res., 34: 661-666.
Zehra, S. and Khan, M.A. 2011. Dietary protein requirement for fingerling Channa punctatus (Bloch), based on growth, feed conversion, protein retention and biochemical composition. Aquacult. Int., 20(2): 383-395. DOI 10.1007/s10499-011-9470-8.