HATAYİ ZENGİN, Nilüfer VURAL, Veysel Kenan ÇELİK

Comparison of changes in fatty acid composition of starved and fed rainbow trout, (Oncorhynchus mykiss) larvae

Aç bırakılan ve beslenen Gökkuşağı alabalığı (Oncorhynchus mykiss) yavrularının yağ asidi kompozisyonundaki değişimler gaz kromatografisi ile belirlenmiştir. Örneklerdeki total lipidler kloroform/metanol (2:1, v/v) karışımında homojenize edilerek elde edilmiştir. Yağ asit metil esterleri total lipitden hazırlanmıştır. Örnek metanollü potasyum hidroksit (KOH) ile sabunlaştırılmıştır. Yağ asitleri metanollü-Boron Trifluoride (metanollü-BF3) ile esterleştirilmiş ve yağ asit metil esterleri Shimadzu GCMSQP5000 marka gaz kromatografisi ile analiz edilmiştir. Beslenen yavrulara kıyasla, aç bırakılan yavrularda, tek çiftbağlı doymamış yağ asitlerinden oleik asit (C18:1ω-9) kullanımı daha fazla tercih edilmiştir. Aynı periyod esnasında, hem aç bırakılan hem de beslenen yavrularda palmitoleik (C16:1ω-7) asit tercihli olarak korunmuştur. 29 gün aç bırakılan yavrularda linolenik asit (C18:3ω-6), eikosanoik asit (C20:0), dokosanoik asit (C22:0) ve dokosatrienoik asit (C22:3ω-3) tükenmiştir. Bu yavrular, eikosapentaenoik (C20:5ω-3; EPA) asidi az kullanmış ve dokosaheksaenoik (C22:6ω-3; DHA) asidi güçlü bir şekilde korumuşlardır.

Aç bırakılan ve beslenen Gökkuşağı alabalığı (Oncorhynchus mykiss) yavrularının yağ asidi kompozisyonundaki değişimlerin karşılaştırılması

Changes in the fatty acid (FA) composition of unfed and fed rainbow trout (Oncorhynchus mykiss) larvae were determined by gas chromatography. Total lipids were extracted from samples by homogenization in chloroform/methanol (2:1, v/v). Fatty acid methyl esters were prepared from total lipid. The sample was saponified with methanolic potassium hydroxide (KOH). The fatty acids esterified with boron trifluoride methanol (BF3 methanol) and fatty acid methyl esters were analyzed with a Shimadzu GCMSQP5000 gas chromatograph.In starved larvae, there was an apparent preference in utilization of oleic acid (C18:1ω-9) in monounsaturated fatty acids than in the fed larvae. In both starved and fed larvae, palmitoleic (C16:1ω-7) acid was preferentially kept during the same period. Larvae kept 29 days under starvation conditions exhausted linolenic acid (C18:3ω-6), eicosanoic acid (C20:0), docosanoic acid (C22:0) and docosatrienoic acid (C22:3ω-3). They utilized less eicosapentaenoic (C20:5ω-3; EPA) acid and conserved strongly docosahexaenoic (C22:6ω-3; DHA) acid.

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