Jian GE, Mingkui LI, Fang LIN, Jin ZHAO, Xianjun DAI

Comparative studies on toxicokinetics and residues of Dimethyl phthalate (DMP) in Tilapia (Oreochromis niloticus × O. aureus) at different water temperatures

Bu çalışmada RP-HPLC yöntemi kullanarak farklı sıcaklık altında (18ºC and 28ºC) tilapia vücudundaki Dimetilfitalat (DMF) kalıntıları ve farmakokinetiği ölçülmüştür. Tilapiyaya oral yolla 20 mg/kg DMP verilmesinden sonra, farklı zaman noktalarındaki plazma, kas, beyin, cilt, kalp’in DMP konsantrasyonu örneği alınıp analiz edilmiştir. Sonuçlara 3p97 farmakokinetik yazılım kullanılarak deneysel verileri analiz edilmiştir. DMP alımı sonrasında Tilapiyalarda yüksek sıcaklık altındaki vücut konsantrasyonu, düşük sıcaklıktan daha yüksek olduğunu göstermektedir. Emilim oranı hızlı emme yarı ömrü 0.020h.dagılım yarı ömrü 0.19 h, eliminasyon yarı ömrü 40.16 h, düşük sıcaklık altındakı emme yarı ömrü ve eliminasyon yarı ömrü ayrı ayrı 3.01 h ve 57.62 h.yüksek sıcaklık altında plazma konsantrasyonu-zaman eğrisi AUC alanı 30.13 μg h $ml^ {-1}$.maksimum zamani 0.074 h. maksimum konsantrasyonu 2.06 μg $ml^ {-1}$;düşük sıcaklık altında ayrı ayrı 74.77 μg h $ml^ {-1}$, 13.51 h ve 0.76 μg $ml^ {-1}$ aynı zamanda farklı su sıcaklıgı altında yedirdikten 360h sonra kas cilt beyin ve kalpdan DMP kalıntıları bulunuyor.ama farklı su sıcaklıgı altındakı Farmakokinetik ve kalıntılar Karşılaştırılma analizi su sıcaklıgının DMP’in tilapia vücudundaki emme,dağılım ve eliminasyona önemli etkisi olduğunu gösteriyor.

Anahtar Kelimeler:

Oreochromis aureus, Oreochromis niloticus, dimetil fitalat, kalıntı, su sıcaklığı, HPLC, Tilapia

Farklı su sıcaklıgı altında Tilapia （Oreochromis niloticus × O. aureus）vücudundaki DMP farmakokinetiği ve kalıntıları karşılaştırılma analizi

The study was conducted to evaluate the toxicokinetic disposition and residue of Dimethyl phthalate (DMP) in tilapia at different water temperatures (18ºC and 28ºC) by RP-HPLC method. After oral administration of DMP with a single does of 20mg/kg body weight, the tilapia’s plasma, muscle, brain, skin and myocardium were sampled separately at different time to determine the concentration of DMP. Then the data were analyzed using 3p97 Pharmacokinetics software written by the Chinese Society of Mathematical Pharmacology, which indicated that the absorption, distribution and elimination half-life at 28ºC were 0.020 h, 0.19 h and 40.16 h, while the absorption half-life and elimination half-time were 3.01 h and 57.62 h at 18ºC. That’s to say, the DMP absorption rate was faster in tilapia at 28ºC than that at 18ºC. And the serum concentration-time curve (AUC) was 30.13μg h $ml^ {-1}$ at 28ºC, while the T-peak was 0.074 hrs and the C-peak were 2.06μg·$ml^ {-1}$; whereas, the corresponding situations at 18ºC were 74.77 μg·h·$ml^ {-1}$, 13.51 hrs and 0.76μg·$ml^ {-1}$, respectively. No matter how, the DMP residue was detected after oral administration for 360 hrs at both water temperatures, but the research had revealed that water temperature had great impact on the DMP absorption, distribution and elimination in tilapia.

Keywords:

Oreochromis aureus, Oreochromis niloticus, dimethyl phthalate, residues, water temperature, HPLC, Tilapia,

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