A 60-day feeding trial was conducted to study the nutrient utilization and growth performance of Labeo rohita fed with T1 [DORB supplemented with phytase and xylanase (0.01% each)], T2 [ T1+ L-lysine(1.4%), L- methionine (0.4%), combination of EPA and DHA (0.5%)] , T3 [DORB supplemented with phytase (0.01%), combination of xylanase and cellulase (0.075%), L-lysine (1.4 %), L- methionine (0.4 %), combination of EPA and DHA (0.5%)] and T4 (commercially available carp feed) diets. Weight gain%, SGR, and FCR were similar (p>0.05) for the fishes fed with either T3 feed or commercial carp feed (T4), whereas PER was highest for the T3 group. A significantly higher apparent dry matter and carbohydrate digestibility were observed in the T3 group. Highest crude protein digestibility was observed in T4 group, though lipid digestibility was similar in both T3 and T4 groups. The activities of digestive enzymes were similar in T2, T3 and T4 groups, whereas the lowest activity of these enzymes were observed in T1 group. Based on the findings it is concluded that T3 feed (crude protein 18.18 %) is having comparable growth performance of Labeo rohita as compared to T4 diet (crude protein 32.01 %) due to higher In vivo digestibility.
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Adeola, O. & Cowieson, A.J. (2011). Opportunities and challenges in using exogenous enzymes to improve non-ruminant animal production. Journal of Animal Science, 89, 3189–3218. http://doi:10.2527/jas.2010-3715
Adeola, O., & Bedford, M. R. (2004). Exogenous dietary xylanase ameliorates viscosity-induced anti-nutritional effects in wheat-based diets for White Pekin ducks (Anas platyrinchos domesticus). British Journal of Nutrition, 92, 87-94. https://doi.org/10.1079/BJN20041180
Adeoye, A. A., Jaramillo-Torres, A., Fox, S. W., Merrifield, D. L., & Davies, S. J. (2016). Supplementation of formulated diets for tilapia (Oreochromis niloticus) with selected exogenous enzymes: Overall performance and effects on intestinal histology and microbiota. Animal Feed Science and Technology, 215, 133-143.
Ai, Q., Mai, K., Zhang, W., Xu, W., Tan, B., Zhang, C., & Li, H. (2007). Effects of exogenous enzymes (phytase, non-starch polysaccharide enzyme) in diets on growth, feed utilization, nitrogen and phosphorus excretion of Japanese seabass, Lateolabrax japonicus. Comparative Biochemistry and Physiology, 147 (B), 502-508. https://doi.org/10.1016/j.cbpa.2007.01.026
Alexis, M.N., & Papaparaskeva-Papoutsoglou, E. (1986). Aminotransferase activity in the liver and white muscle of Mugil capito fed diets containing different levels of protein and carbohydrate. Comparative Biochemistry and Physiology, 83B:245–249. https://doi.org/10.1016/0305-0491(86)90361-5
Alloui, O., Smulikowska, S., Chibowska, M., & Pastuszewska, B. (1994). The nutritive value of lupin seeds (L. luteus, L . angustifolius and L . albus) for broiler chickens as affected by variety and enzyme supplementation. Journal of Animal and Feed Sciences, 3, 215-227. https://doi.org/10.22358/jafs/69836/1994
AOAC (Association of Official Analytical Chemists)(1995). Official Methods of Analysis of the Association Official Analytical Chemists, 16th ed. AOAC, Inc., Arlington, Virginia, USA.
Bedford, M., & Cowieson, A. (2012). Exogenous enzymes and their effects on intestinal microbiology. Animal Feed Science and Technology, 173, 76–85. https://doi.org/10.1016/j.anifeedsci.2011.12.018
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Cao, L., Wang, W., Yang, C., Yang, Y., Diana, J., Yakupitiyage, A., Luo, Z., & Li, D. (2007). Application of microbial phytase in fish feed. Enzyme and Microbial Technology, 40, 497–507. https://doi.org/10.1016/j.enzmictec.2007.01.007
Cao, L., Yang, Y., Wang, W., Yakupitiyage, A., Yuan, D., & Diana, J. (2008). Effects of pre-treatment with microbial phytase on phosphorous utilization and growth performance of Nile tilapia (Oreochromis niloticus). Aquaculture Nutrition, 14, 99–109. https://doi.org/10.1111/j.1365-2095.2007.00508.x
Cao, L., Yang, Y., Wang, W., Yakupitiyage, A., Yuan, D., & Diana, J. (2008). Effects of pre-treatment with microbial phytase on phosphorous utilization and growth performance of Nile tilapia (Oreochromis niloticus). Aquaculture Nutrition, 14, 99–109. https://doi.org/10.1111/j.1365-2095.2007.00508.x
Cho, C.Y., Slinger, S.J., (1979). Apparent digestibility measurement in feedstuffs for rainbow trout. In: Halver, J., Tiews, K. (Eds.), Proc. World Symposium on Finfish nutrition and fish feed technology vol. 2. Heenemann, Berlin, 239- 247
Classen, H. L., (1996). Cereal grain starch and exogenous enzymes in poultry diets. Animal Feed Science and Technology, 62, 21-27. https://doi.org/10.1016/S0377-8401(96)01002-4
Drapeau, G., (1974). Protease from Staphylococcus aureus. In: Lorand, B.L. (Ed.), Methods in Enzymology. Academic Press, NY, USA, p. 469 pp
Enes, P., Panserat, S., Kaushik, S., & Oliva-Teles, A. (2006). Effect of normal and waxy maize starch on growth, food utilization and hepatic glucose metabolism in European sea bass (Dicentrarchus labrax) juveniles. Comparative Biochemistry and Physiology, 143A, 89–96. https://doi.org/10.1016/j.cbpa.2005.10.027
Esonu, B. O., Izukanne, R. O., & Inyang, O. A. (2005). Evaluation of cellulolytic enzyme supplementation on production indices and nutrient utilization of laying hens fed soybean hull based diets. International Journal of Poultry Science, 4(4), 213-216.
Espe, M., Lemme, A., Petri, A., & El-Mowafi, A. (2006). Can Atlantic salmon (Salmo salar) grow on diets devoid of fish meal? Aquaculture, 255, 255-262. https://doi.org/10.1016/j.aquaculture.2005.12.030
Farhangi M., & Carter C.G. (2007). Effect of enzyme supplementation to dehulled lupin-based diets on growth, feed efficiency, nutrient digestibility and carcass composition of rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, 38, 1274– 1282. https://doi.org/ 10.1111/j.1365- 2109.2007.01789.x
Fawole, F. J., Sahu, N. P., Jain, K. K., Gupta, S., Shamna, N., Phulia, V., & Prabu, D. L. (2016). Nutritional evaluation of protein isolate from rubber seed in the diet of Labeo rohita: Effects on growth performance, nutrient utilization, whole body composition andmetabolic enzymes activity. Animal Feed Science and Technology, 219, 189-199. https://doi.org/10.1016/j.anifeedsci.2016.06.014
Figueiredo‐ Silva, C., Lemme, A., Sangsue, D., & Kiriratnikom, S. (2015). Effect of DL‐ methionine supplementation on the success of almost total replacement of fish meal with soybean meal in diets for hybrid tilapia (Oreochromis niloticus× Oreochromis mossambicus). Aquaculture Nutrition, 21, 234-241. https://doi.org/10.1111/anu.12150
Furukawa, A., & Tsukahara, H. (1966). On the acid digestion method for the determination of chromic oxide as the index substance in the study of fish feed. Bulletin of the Japanese Society for the Science of Fish, 32, 502–506.
Folch, J., Lees, M., Sloane-Stanley, G. H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226(1), 497-509.
Fynn-Aikins, K., Hung, S. S., & Hughes, S. G. (1993). Effects of feeding a high level of D-glucose on liver function in juvenile white sturgeon (Acipenser transmontanus). Fish Physiology and Biochemistry, 12, 317-325. https://doi.org/10.1007/BF00004416
Gatlin, D. M., Barrows, F. T., Brown, P., Dabrowski, K., Gaylord, T. G., Hardy, R. W., Herman, E., Hu, G., Krogdahl, Å., Nelson, R., Overturf, K., Rust, M., Sealey, W., Skonberg, D., J Souza, E., Stone, D., Wilson, R., & Wurtele, E. (2007). Expanding the utilization of sustainable plant products in aquafeeds: a review. Aquaculture Research, 38,551–579. https://doi.org/10.1111/j.1365-2109.2007.01704.x
Ghazi, S., Rooke, J. A., & Galbraith, H. (2003). Improvement of the nutritive value of soybean meal by protease and a-galactosidase treatment in broiler cockerels and broiler chicks. British Poultry Science, 44, 410-418. https://doi.org/10.1080/00071660310001598283
Ghomi, M. R., Shahriari, R., Langroudi, H. F., Nikoo, M., & von Elert, E. (2012). Effects of exogenous dietary enzyme on growth, body composition, and fatty acid profiles of cultured great sturgeon Huso huso fingerlings. Aquaculture International, 20, 249-254. https://doi.org/10.1007/s10499-011-9453-9
Jiang, T. T., Feng, L., Liu, Y., Jiang, W. D., Jiang, J., Li, S. H., & Zhou, X. Q. (2014). Effects of exogenous xylanase supplementation in plant protein‐ enriched diets on growth performance, intestinal enzyme activities and microflora of juvenile Jian carp (Cyprinus carpio var. jian). Aquaculture Nutrition, 20, 632-645. https://doi/10.1111/anu.12125/
Kiarie, E., Romero, L.F., & Nyachoti, C.M. (2013). The role of added feed enzymes in promoting gut health in swine and poultry. Nutrition Research Reviews, 26, 71–88.
Kumar, S., Sahu, N. P., Pal, A. K., Choudhury, D., & Mukherjee, S. C. (2006). Studies on digestibility and digestive enzyme activities in Labeo rohita (Hamilton) juveniles: effect of microbial α-amylase supplementation in non-gelatinized or gelatinized corn-based diet at two protein levels. Fish Physiology and Biochemistry, 32, 209-220. https://doi.org/10.1007/s10695-006-9002-z
Kumar, S., Sahu, N. P., Pal, A. K., Sagar, V., Sinha, A. K., & Baruah, K. (2009). Modulation of key metabolic enzyme of Labeo rohita (Hamilton) juvenile: effect of dietary starch type, protein level and exogenous α-amylase in the diet. Fish Physiology and Biochemistry, 35, 301-315. https://doi.org/10.1007/s10695-008-9213-6
Kumar, S., Sahu, N.P., Pal, A.K., Choudhury, D., & Mukherjee, S.C., (2006a). Non-gelatinized corn supplemented with a-amylase at sub-optimum protein level enhances the growth of Labeo rohita (Hamilton) fingerlings. Aquaculture Research, 37, 284-292. https://doi.org/10.1111/j.1365-2109.2005.01434.x
Kumar, V., Sahu, N. P., Pal, A. K., Kumar, S., Sinha, A. K., Ranjan, J., & Baruah, K. (2010). Modulation of key enzymes of glycolysis, gluconeogenesis, amino acid catabolism, and TCA cycle of the tropical freshwater fish Labeo rohita fed gelatinized and non-gelatinized starch diet. Fish Physiology and Biochemistry, 36, 491-499. https://doi.org/10.1007/s10695-009-9319-5
Li, P., Mai, K., Trushenski, J., & Wu, G. (2009). New developments in fish amino acid nutrition: towards functional and environmentally oriented aquafeeds. Amino Acids, 37, 43-53. https://doi.org/10.1007/s00726-008-0171-1
Lin S., Mai K. & Tan B., (2007). Effects of exogenous enzyme supplementation in diets on growth and feed utilization in tilapia, Oreochromis niloticus × Oreochromis aureus. Aquaculture Research, 38, 1645–1653. https://doi.org/10.1111/j.1365- 2109.2007.01825.x
Magalhães, R., Lopes, T., Martins, N., Díaz-Rosales, P., Couto, A., Pousão-Ferreira, P., Oliva-Teles A., & Peres, H. (2016). Carbohydrases supplementation increased nutrient utilization in white seabream (Diplodus sargus) juveniles fed high soybean meal diets. Aquaculture, 463, 43-50. https://doi.org/10.1016/j.aquaculture.2016.05.019
Mai, K., Zhang, L., Ai, Q., Duan, Q., Zhang, C., Li, H., Wan, J.L., & Liufu, Z.G. (2006). Dietary lysine requirement of juvenile Japanese seabass, Lateolabrax japonicus. Aquaculture, 258, 535-542. https://doi.org/10.1016/j.aquaculture.2006.04.043
Misra, S., Sahu, N. P., Pal, A. K., Xavier, B., Kumar, S., & Mukherjee, S. C. (2006). Pre-and post-challenge immuno-haematological changes in Labeo rohita juveniles fed gelatinised or non-gelatinised carbohydrate with n-3 PUFA. Fish & Shellfish Immunology, 21, 346-356. https://doi.org/10.1016/j.fsi.2005.12.010
Mukhopadhayay, N., & Ray A.K. (1999). Improvement of quality of sal (Shorea robusta) seed meal protein with supplemental amino acids in feed for rohu, Labeo rohita (Hamilton), fingerlings. Acta Ichthyology Fiscal, 29, 25–39.
Mukhopadhyay, N. (2000). Improvement of quality of copra (dried kernel of Cocos nucifera) seed meal protein with supplemental amino acids in feeds for rohu, Labeo rohita (Hamilton) fingerlings. Acta Ichthyology Fiscal, 30, 21–34.
Ng, W. K., & Chen, M. L. (2002). Replacement of soybean meal with palm kernel meal in practical diets for hybrid Asian-African catfish, Clarias macrocephalus × C. gariepinus. Journal of Applied Aquaculture, 12, 67-76. https://doi.org/10.1300/J028v12n04_06
Ng, W. K., Keembiyehetty, C. N., & Wilson, R. P. (1998). Bioavailability of niacin from feed ingredients commonly used in feeds for channel catfish, Ictalurus punctatus. Aquaculture, 161, 393-404. https://doi.org/10.1016/S0044-8486(97)00287-1
Ogunkoya, A.E., Page, G.I., Adewolu, M.A., & Bureau, D.P. (2006). Dietary incorporation of soybean meal and exogenous enzyme cocktail can affect physical characteristics of faecal material egested by rainbow trout (Oncorhynchus mykiss). Aquaculture, 254, 466– 475. https://doi.org/10.1016/j.aquaculture.2005.10.032
Ramakrishna, R., Shipton, T. A., & Hasan, M. R. (2013). Feeding and feed management of Indian major carps in Andhra Pradesh, India. FAO Fisheries and Aquaculture Technical Paper No. 578, p. 90
Ranjan, A., Sahu, N. P., Deo, A. D., Kumar, H. S., Kumar, S., & Jain, K. K. (2017). Xylanase and Phytase Supplementation in the De-oiled Rice Bran (DORB) based Diet Improves the Growth Performance of Labeo rohita. International Journal of Current Microbiology and Applied Science, 6(6), 1493-1503. https://doi.org/10.20546/ijcmas.2017.606.176
Rick, W. & Stegbauer, H.P. (1974). Amylase measurement of reducing groups. In: Bergmeyer, H.V. (Ed.), Methods of Enzymatic Analysis, vol. 2, 2nd ed. Academic Press, New York, pp. 885–889
Sardar, P., Abid, M., Randhawa, H. S., & Prabhakar, S. K. (2009). Effect of dietary lysine and methionine supplementation on growth, nutrient utilization, carcass compositions and haemato‐ biochemical status in Indian Major Carp, Rohu (Labeo rohita H.) fed soy protein‐ based diet. Aquaculture Nutrition,15, 339-346. https://doi.org/10.1111/j.1365- 2095.2008.00598.x
Sargent, J. R., Bell, J. G., Bell, M. V., Henderson, R. J., & Tocher, D. R. (1995). Requirement criteria for essential fatty acids. Journal of Applied Ichthyology, 11, 183-198. https://doi.org/10.1111/j.1439- 0426.1995.tb00018.x
Sargent, J., Bell, G., McEvoy, L., Tocher, D., & Estevez, A. (1999). Recent developments in the essential fatty acid nutrition of fish. Aquaculture, 177, 191-199. https://doi.org/10.1016/S0044-8486(99)00083-6
Sargent, J.R., Bell, J.G., Bell, M.V., Henderson, R.J., & Tocher, D.R. (1993). The Metabolism of Phospholipids and Polyunsaturated Fatty Acids in Fish, in Aquaculture: Fundamental and Applied Research (eds B. Lahlou and P. Vitiello), American Geophysical Union, Washington, D. C. pp 103-124. https://doi.org/10.1029/CE043p0103
Shiau, S .Y., & Liang, H.S. (1994). Carbohydrate utilization and digestibility by tilapia Oreochromis niloticus × O. aurous are affected by chromic oxide inclusion in the diet. Journal of Nutrition, 125, 976-982
Soltan, M.A. (2009). Effect of dietary fishmeal replacement by poultry by- product meal with different grain source and enzyme supplementation on performance, faeces recovery, body composition and nutrient balance of Nile tilapia. Pakistan Journal of Nutrition, 8, 395–407.
Tacon, A. (1994). Feed ingredients for carnivorous fish species: alternatives to fishmeal and other fishery resources. FAO Fisheries Circular, 881, 35.
Usmani, N., Jafri, A.K. & Khan, M.A. (2003). Nutrient digestibility studies in Heteropneustes fossilis (Bloch), Clarias batrachus (Linnaeus) (Burchell) and C. gariepinus. Aquaculture Research, 34, 1247-1253. https://doi.org/10.1046/j.1365-2109.2003.00930.x
Van Weerd, J.H., Khalaf, K.A., Aartsen, F.J., & Tijssen, P.A.T. (1999). Balance trials with catfish Clarias gariepinus fed phytase treated soybean meal-based diets. Aquaculture Nutrition, 5, 135–142.https://doi.org/10.1046/j.1365-2095.1999.00100.x
Vandeningh, T., Olli, J., & Krogdahl, Å. (1996). Alcohol‐ soluble components in soybeans cause morphological changes in the distal intestine of Atlantic salmon, Salmo salar L. Journal of Fish Diseases, 19, 47-53. https://doi.org/10.1111/j.1365- 2761.1996.tb00119.x
Walton, M.J., & Cowey, C.B. (1982). Aspects of intermediary metabolism in salmonid fish. Comparative Biochemistry and Physiology, 73B, 59– 79. https://doi.org/10.1016/0305-0491(82)90201-2
Xavier, B., Sahu, N.P., Pal, A.K., Jain, K.K., Misra, S., Dalvi, R.S., & Baruah, K. (2012). Water soaking and exogenous enzyme treatment of plant-based diets: effect on growth performance, whole-body composition, and digestive enzyme activities of rohu, Labeo rohita (Hamilton), fingerlings. Fish Physiology and Biochemistry, 38, 341–353. https://doi.org/10.1007/s10695-011-9511-2
Yiğit, N.O., Koca, S.B., Didinen, B.I., & Diler, I. (2014). Effect of β-mannanase and α-Galactosidase supplementation to soybean meal based diets on growth, feed efficiency and nutrient digestibility of rainbow trout, Oncorhynchus mykiss (Walbaum). Asian-Australasian Journal of Animal Science, 27, 700–705. https://dx.doi.org/10.5713%2Fajas.2013.13616
Zamini, A., Kanani, H., Esmaeili, A., Ramezani, S., & Zoriezahra, S. (2014). Effects of two dietary exogenous multi-enzyme supplementation, Natuzyme® and beta-mannanase (Hemicell®), on growth and blood parameters of Caspian salmon (Salmo trutta caspius). Comparative Clinical Pathology, 23, 187-192. https://doi.org/10.1007/s00580-012-1593-4 Zhou, Y., Jiang, Z., Lv, D., & Wang, T. (2009). Improved energy-utilizing efficiency by enzyme preparation supplement in broiler diets with different metabolizable energy levels. Poultry Science, 88, 316- 322. https://doi.org/10.3382/ps.2008-00231
Zhou, Y., Yuan, X., Liang, X.-F., Fang, L., Li, J., Guo, X., Bai, X., & He, S. (2013). Enhancement of growth and intestinal flora in grass carp: the effect of exogenous cellulase. Aquaculture, 416, 1–7. https://doi.org/10.1016/j.aquaculture.2013.08.023
Zijlstra, R., Owusu-Asiedu, A., & Simmins, P. (2010). Future of NSP-degrading enzymes to improve nutrient utilization of co-products and gut health in pigs.Livestock Science, 134,255–257. https://doi.org/10.1016/j.livsci.2010.07.017