Muhammed Bahattin TANYOLAC, Volkan EROGLU, Seda NEMLI, Serdar Gokhan SENOL, Umit SUBASI

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HIGH LEVELS OF GENETIC VARIATION AS DETECTED BY AFLP IN Sideritis tmolea FROM WESTERN TURKEY

HIGH LEVELS OF GENETIC VARIATION AS DETECTED BY AFLP IN Sideritis tmolea FROM WESTERN TURKEY

The objective of this study was to evaluate the level of genetic diversity among 29 S. tmolea genotypes collected from Bozdag, Turkey, using the Amplified Fragment Length Polymorphism (AFLP) technique. A total of 392 AFLP markers were detected using 14 primer combinations. The number of polymorphic bands per AFLP primer combination ranged from 16 to 42, with an average of 28. The Polymorphism Information Content (PIC) values varied from 0.25 (M-CTC/E-AGC) to 0.72 (M-CAC/E-AAG) among 14 selective primers. The genetic dissimilarity that was detected using the NTSYS-PC software ranged from 0.09 to 0.82. According to the dissimilarity results, a high level of genetic diversity existed among the studied genotypes. A model-based structural analysis revealed the presence of 2 populations. The defined population structure was helpful when studying the S. tmolea genotypes for diversity and classification
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  • Akcos, Y., N. Ezer, I. Calıs, R. Demirdamar, B.C. Tel. 1999. Polyphenolic compounds of Sideritis lycia and their antiinflammatory activity. Pharm Biol. 36: 1-5.
  • Astarini, A.I., A.J. Plummer, A.R. Lancaster and G. Yan. 2004. Fingerprinting of cauliflower cultivars using RAPD markers. Aust J Agri Res. 55: 112-124.
  • Baser, K.H.C., G. Honda and W. Miki. 1986. Herb Drugs and Herbalists in Turkey- Studia Culturae Islamicae 27, Institute for the Study of Languages and Cultures of Asia and Africa, Tokyo.
  • Biton, I., S. Shevtsov, O. Ostersetzer, Y. Mani, S. Lavee, B. Avıdan and G. Ben-ari, 2012. Genetic relationships and hybrid vigour in olive (Olea europaea L.) by Microsatellites. Plant Breeding. 131: 767-774.
  • Bondi, M.L., M. Bruno, F. Piozzi, K.H.C. Baser and M.S.J. Simmonds. 2000. Diversity and antifeedant activity of diterpenes from Turkish species of Sideritis. Biochem Sys Ecol. 28: 299-303.
  • Brummer, E.C., J.H. Bouton and G. Korchert. 1995. Analysis of annual Medicago species using RAPD markers. Genome. 38: 362-367.
  • Cho, G-T., J. Lee, J-K. Moon, M-S. Yoon, H-J. Baek, J-H. Kang, T-S. Kim and N-C. Paek. 2008. Genetic diversity and population structure of Korean soybean landrace [Glycine max (L.) Merr.]. J Crop Sci Biotech. 11(2): 83-90.
  • Cinar, A., S. Elmasulu, A.G. Ince, M. Karaca, A.N. Onus and K. Turgut. 2009. A suitable DNA marker technique (minisatellites) for Sideritis (Lamiaceae) genotyping studies. Acta Horticulturae. 826(1): 439-446.
  • Colomba, M.S. and A. Gregorini. 2011. Genetic diversity L. subsp. durum (Desf.) Husn. (Poales, Poaceae). Biodiversity Journal. 2(2): 73-84.
  • Cui, H., K.T. Moe, J.W. Chung, Y.I. Cho, G.A. Lee and Y.J. Park. 2010. Genetic diversity and population structure of rice accessions from South Asia using SSR markers. Korean Journal of Breeding Science. 42(1): 11-22.
  • Davis, P.H. 1978. Flora of Turkey and the East Aegean Islands. Vol. 6, Edinburgh.
  • Doyle, J.J. and J.L .Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull. 19: 11–15.
  • Duman, H. 2013. Sideritis. ed. Güner, A., S. Aslan, T. Ekim, M. Vural and M.T. Babaç, Nezahat Gokyigit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, Istanbul.
  • Evanno, G., S. Regnaut and Goudet, J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Molecular Ecology. (14)8: 2611-2620.
  • Farah Fazwa, M.A., H. Siti Salwana, H. Maideen and O. Mohamad. 2013. An Assessment of Genetic Relationship Among Superior Accessions of Labisia Pumila Analized by Amplified Fragment Length Polymorphism (AFLP) Markers. Open Science Repository Agriculture. Online(open-access). doi:10.7392/Agriculture.70081945.
  • Grati-Kamoun, N., F. Lamy Mahmoud, A. Rebai, A. Gargouri, O. Panaud and A. Saar. 2006. Genetic diversity of Tunisian olive tree (Olea europaea L.) cultivars assessed by AFLP markers. Genet Resour Crop Ev. 53: 265–275.
  • Gonza´lez-Pe´rez, M., P.A Asosa and F.J. Batista. 2008. Genetic variation and conservation of the endangered endemic Anagyris latifolia Brouss. ex Willd. (Leguminosae) from the Canary Islands. Plant Syst Evol. doi 10.1007/s00606-009- 0146-z.
  • Guner, A., N. Ozhatay, T. Ekim and K.H.C. Baser. 2000. Flora of Turkey and the East Aegean Islands. Edinburgh: Edinburgh University Press. 11(2): 35-37.
  • Heywood, V.H. 1996. Flowering Plant Of The World - BT Bastrford LTD, London, pp:239.
  • Hickey, M. and C. King. 1997. Common Families of Lowering Plants. Chambridge Univ. Press., England, pp: 119-127.
  • Jaccard, P. 1908. Nouvelles recherches sur la distribution florale. Bul. Soc. Vaudoise Sci. Nat. 44: 223–270.
  • Khalighi, M., A. Arzani and M.A. Poursiahbidi. 2008. Assessment of genetic diversity in Triticum spp. and Aegilops spp. using AFLP markers. Afri J Biotechn. 7(5): 546-552.
  • Kirimer, N., N. Tabanea and B. Demirci. et al. 2001. The essential oil of a new Sideritis species: Sideritis ozturkii. Chem Nat Compd. 37(3): 234-237.
  • Lapitan, V.C., D.S. Brar, T. Abe and E.D. Redona. 2007. Assessment of genetic diversity of Philippine rice carrying good quality traits using SSR markers. Breeding Sci. 57: 263-270.
  • Maciel, F.L., S. Echeverrigaray, L.T.S. Gerald and F.G. Grazziotin. 2003. Genetic relationships and diversity among Brazilian cultivars and landraces of common beans (Phaseolus vulgaris L.) revealed by AFLP markers. Genetic Resources and Crop Evolution. 50: 887–893.
  • Maras, M., J. Sustar-Vozlic, B. Javornik and V. Meglic. 2008. The efficiency of AFLP and SSR markers in genetic diversity estimation and gene pool classification of common bean (Phaseolus vulgaris L.). Acta Agric Slov. 91: 87–96.
  • Martins-Lopes, P.S., G.E. Santos and H. Guedes-Pinto. 2008. DNA markers for Portuguese olive oil fingerprinting. J Agric Food Chem. 56: 11786-11791.
  • Mudibu, J., K.K.C. Nkongolo, M. Mehes-Smith and A. Kanlonji-Mbuyi 2011. Genetic analysis of a soybean genetic pool using ISSR markers: Effect of gamma radiation on genetic variability. Plant Breeding Genet. 5: 235-245.
  • Murtaza, N. 2006. Cotton genetic diversity study by AFLP markers. Electronic Journal of Biotechnology ISSN: 0717- 3458, 9(4), Issue of July 15.
  • Muse, S.V. and K.J. Liu. 2005. Powermarker: An integrated analysis environment for genetic marker analysis. Bioinformatics. 21: 2128- 2129.
  • Nemli, S. 2013. Detection of DNA Markers Controlling Some Economically Important Agronomic Characters by Association Mapping in Common Bean (Phaseolus vulgaris L.) - Dissertation, University of Ege, Izmir, Turkey .
  • Nybom, H. and I.V. Bartish. 2000. Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspect Plant Ecol Evol Syst. 3: 93–114.
  • Ozturk, Y., S. Aydın, N. Ozturk and K.H.C. Baser. 1996. Effects of extracts from certain Sideritis species on swimming performance in mice. Phytother Res. 10: 70-73.
  • Ozhatay, N., M. Koyuncu, S. Atay and A. Byfield. 1998. The trade in wild medicinal plants in Turkey. In „Medicinal plant trade in Europe: conservation and supply‟, eds Traffıc Europe pp. 5-18 - Proceeding of the First International Symposium on the Conservation of Medicinal Plants in Trade in Europe. TRAFFIC Europe, Brussels.
  • Panahi, B., R. Afzal, M.G. Neghab, M. Mahmoodnia and B. Paymard. 2013. Relationship among AFLP, RAPD marker diversity and Agromorphological traits in safflower (Carthamus tinctorius L.) - Progress in Biological Sci. pp 90-99.
  • Pecina-Quintero, V., J.L. Anaya-López, A. ZamarripaColmenero, N. Montes-García, C. Nuñez-Colín, J.L. SolisBonilla and et al. 2012. Genetic diversity of sweet sorghum germplasm in Mexico using AFLP and SSR markers. Pesqui Agropecu Bras. 47: 1095–1102.
  • Pritchard, J.K., M. Stephens and P. Donnelly. 2000. Inference of population structure using multilocus genotype data. Genetics. 155(2): 945-959.
  • Prohens, J., G.J. Anderson, F.J. Herraiz, G. Bernardello, A. Santos-Guerra, D. Crawford and , F. Nuez. 2007. Genetic diversity and conservation of two endangered eggplant relatives (Solanum verpertilio Aiton, and Solanum lidii Sunding) endemic to the Canary Islands. Genet Resour Crop Evol. 54: 451–464
  • Qian, X., C-X. Wang and M. Tian. 2013. Genetic diversity and population differentiation of Calanthe tsoongiana, a rare and endemic orchid in China. Int J Mol Sci. 14: 20399-20413.
  • Rohlf, F.J. 1998. NTSYSpc Numerical Taxonomy and Multivariate Analysis System Version 2.0. User Guide. Applied Biostatistics Inc., Setauket, New York, pp.37.
  • Ryding, O. 1994. The importance of pericarp structure in the classification of Labiatae. Lamiales Newsletter 3: 1-3.
  • Saiki, R.K., D.H. Gelfand, S. Stoffel, S.J. Scharf, R. Higuchi, G.T. Horn, K.B. Mullis and H.A. Erlich. 1988. Primerdirected enzymatic amplification of DNA with thermostable DNA polymerase. Science. 239: 487-91.
  • Shimomura, K. and K. Hirashima, 2006. Development and characterization of simple sequence repeats (SSR) as marker to identify strawberry cultivars (Fragiria x ananassa Duch.). Journal of the Japanese Society for Horticultural Sci. 75(5): 399-402.
  • Smith, J.S.C., E.C.L. Chin, H. Shu, O.S. Smith, S.J. Wall, M.L. Senior, S.E. Mitchel, S. Resorich and J. Tiegle. 1997. An evaluation of the utility of SSR loci as molecular marker in maize Zea mays): comparisons with data from RFLP and pedigree. Theor Appl Genet. 95: 163-173.
  • Strikic, F., D.B. Mavsar, S. Perica, Z. Cmelik, Z. Satovic and B. Javornik. 2010. Genetic variation within the olive (Olea europaea L.) cultivar Oblica detected using amplified fragment length polymorphism (AFLP) markers. African Journal of Biotechnology. 9(20): 2880-2883.
  • Topcu, G., A.C. Goren, T. Kilic, Y.K. Yildiz and G. Tumen. 2002. Diterpenes from Sideritis trojana. Nat Prod Lett. 16: 33–3.
  • Vuylsteke, M., R. Mank, R. Antonise, E. Bastiaans, M.L. Senior, C.W. Stuber, A.E. Melchinger and et al. 1999. Two highdensity AFLP®linkage maps of Zea mays L.: analysis of distribution of AFLP markers. Theor Appl Genet. 99: 921- 935.
  • Witkowicz, J., E. Urbanczyk-Wochniak and Z. Przybecki. 2003. AFLP marker polymorphism in cucumber (Cucumis sativus L.) near isogenic lines differing in sex expression. Cell Mol Biol Lett. 8: 857-857.
  • Yuan, W.J., J.H. Yuan, M.F. Dong and F.D. Shang. 2011. Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers. Electronic Journal of Biotechnology. ISSN: 0717-3458 http://www.ejbiotechnology.info. doi: 10.2225/vol14-issue1- fulltext-9.
  • Zhang, L., Y-J. Jeon, S-Y. Kang and G-J. Lee. 2012. Genetic Diversity of Natural and Artificial Populations of Model Grass Brachypodium Species Evaluated by AFLP Markers. Hort Environ Biotechnol. 53(2): 143-150.
Turkish Journal Of Field Crops-Cover
  • ISSN: 1301-1111
  • Yayın Aralığı: Yılda 2 Sayı
  • Başlangıç: 1996
  • Yayıncı: Tarla Bitkileri Bilimi Derneği
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