Ogunc MERAL, Mert PEKCAN, Elçin ÖZGÜR, Görkem KISMALI, Deniz DEMİRKIRAN, Göknur GÜLER ÖZTÜRK, Nesrin SEYHAN

Baz istasyonundan yayılan 3G cep telefonu radyasyonunun hepatoselüler karsinoma hücre hattında 14-3-3 Protein Ailesine etkisi

Amaç: Mobil telekomünikasyon servislerindeki yaygın artış, halka açık alanlar ve konutlardaki radyofrekans radyasyon (RFR) miktarının başlıca sorumlusudur. Üçüncü nesil (3G) sistem teknolojileri ikinci nesil (2G) teknolojilere göre taşıyıcı frekansları bakımından oldukça farklıdırlar. 14-3-3 protein ailesi metabolik yolların düzenlenmesinde önemli rollere sahip hücresel proteinleri içerir. Bununla beraber 14-3-3 protein ailesi hücre büyümesi, farklılaşması ve apoptotik hücre ölümü gibi birçok hücresel süreçte görev alırlar. Çalışmanın amacı 3G cep telefonu baz istasyonundan yayılan radyofrekans radyasyona maruz kalan hepatoselüler karsinoma hücrelerinde (HepG2) 14-3-3 protein ekspresyonunun incelenmesidir. Gereç ve Yöntemler: HepG2 hücreleri 1, 2, 3, ve 4 saat olmak üzere dört farklı periyotta radyofrekans radyasyona maruz bırakılmış ve protein ekspresyonundaki farklılıkların incelenmesi için western blot analizi gerçekleştirilmiştir.Bulgular: Kontrol, sham ve maruziyet gruplarında 14-3-3 protein ekspresyonunda değişime rastlanmamıştır. Sonuçlar: Bu çalışmada üçüncü nesil cep telefonu baz istasyonundan yayılan radyasyonun 14-3-3 protein ekspresyonuna etkisi ile ilgili veriler elde edilmiştir. 14-3-3 izoenzim analizleri ve uzun süreli maruziyeti içeren çalışmaların yapılması ile üçüncü nesil cep telefonundan yayılan radyasyonun HepG2 hücre hattına etkisinin daha iyi anlaşılmasına olanak sağlanacaktır.

Anahtar Kelimeler:

14-3-3 protein ailesi, Hepatoselüler karsinom, Radyofrekans

The Impact of 3G Mobile Phone Base Station Radiation on 14-3-3 Family Proteins in Hepatocellular Carcinoma Cell Line

Aim: The widespread increase of mobile telecommunication services is mainly responsible for the amount of radiofrequency radiation (RFR) in public and residential areas. Third generation (3G) is different from second generation (2G) system technologies with respect to carrier frequency. 14-3-3 proteins are a family of highly conserved cellular proteins that plays a crucial role in the regulation of metabolic pathways. Besides 14-3-3 proteins regulate many cellular processes such as cell growth, differentiation and apoptotic cell death. The aim was to study the possible effects of third generation mobile phone base station like Radiofrequency Radiation exposure on the 14-3-3 protein expression in the hepatocellular carcinoma cells (HepG2). Materials and Methods: HepG2 cells were exposed RFR in four different periods as 1 hour, 2, 3 and 4 hours and then western blot analysis was performed to analyse protein expression level.Results: Expressions of 14-3-3 protein were found unaltered in the control, sham and exposure groups. Conclusion: This report presents data about the effects of 3G system technologies mobile phone base station radiation on 14-3-3 protein expression. 14-3-3 isoenzyme analysis and long term exposure experiments should be done to understand the exact effects of 3G mobile phone radiation on HepG2 cell line.

Keywords:

14-3-3 protein family, Hepatocellular carcinoma, Radiofrequency,

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