Ortopedik implantlar üzerinde kolonize olan biyofilm üreten patojenler üzerinde bakırın bakterisidal ve antibiyofilm aktivitesi

Amaç: Antibiyotik direnci kolaylıkla geliştiğinden beri, yeni, alternatif antimikrobiyal ve antibiyofilm ajan keşfi dikkat çekmektedir. Metallerin antimikrobiyal aktivitelere sahip olduğu kabul edilmiştir. Bakır ile emdirilmiş ortopedik implantlar gibi abiyotik yüzeyler, biyofilm üreten patojenlerin kolonizasyonunu önleyebilir ve implant üzerinde oluşturulan biyofilmleri ayırabilir. Bu çalışmada, bakırın planktonik bakteriler ve kirschner teli ortopedik implantı üzerine yapışan biyofilme gömülü bakterilere karşı etkisi çalışıldı. Gereç ve Yöntemler: Kirschner teli ortopedik implant üzerinde kolonize olan metisilin dirençli Staphylococcus aureus (MRSA), metisilin duyarlı Staphylococcus aureus (MSSA), metisilin dirençli Staphylococcus epidermidis (MRSE), metisilin duyarlı Staphylococcus epidermidis (MSSE), Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa), Proteus mirabilis (P. mirabilis) gibi ana biyofilm oluşturan patojenler üzerinde bakırın MIK, MBK ve MBEK değerleri belirlendi.Bulgular: Bakırın, patojenlere karşı MIK, MBK ve MBEK değerleri 0.063 - 0.75 mg/mL arasında değişmektedir. Bu çalışma, 0.75 mg/mL bakırın çalışmada analiz edilen tüm izolatları inhibe ettiğini göstermiştir. En dirençli patojen MRSA idi. Bakırın biyofilme gömülü bakteriler ve planktonik bakteriler üzerindeki aktivitesi aynıydı. Sonuç: Ortopedik teller, protezler gibi yabancı medikal cisimler, yabancı cisimler üzerinde kolonizasyon ve olgun biyofilm oluşturulmasını önlemek için bakır ile emdirilebilir.

Anahtar Kelimeler:

Biyofilm, biyofilme gömülü patojenler, bakır, implantlar, antibiyofilm, antimikrobiyal

Bactericidal and antibiofilm activities of copper against biofilm producer pathogens colonized on orthopedic implants

Background: New and alternative antimicrobial and antibiofilm agent discovery has gained attention since antibiotic resistance was easily developed. It has been accepted that metals have antimicrobial activity. Abiotic surfaces such as orthopedic implants that are impregnated with copper can prevent colonization of biofilm producer pathogens, and can detach biofilms produced on implants. In this study, the effects of copper against planktonic bacteria and biofilm embedded bacteria adhered on kirschner wire orthopedic implant were studied. Material and Methods: MICs, MBCs and MBEC of copper against main biofilm producer pathogens such as methicillin resistance Staphylococcus aureus (MRSA), methicillin sensitive Staphylococcus aureus (MSSA), methicillin resistance Staphylococcus epidermidis (MRSE), methicillin sensitive Staphylococcus epidermidis (MSSE), Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa), Proteus mirabilis (P. mirabilis) colonized on kirschner wire orthopedic implant were determined.Results: MICs, MBCs, and MBECs of copper against pathogens were ranged from 0.063 to 0.75 mg/mL. This study revealed that 0.75 mg/mL of copper inhibit all isolates analyzed in this study. The most tolerant pathogen was MRSA. The activities of copper against biofilm embedded bacteria and planktonic bacteria were found to be the same.Conclusion: Indwelling medical devices such as orthopedic wires, prosthetics can be impregnated by copper to overcome colonization and production of matured biofilm on indwelling devices, consequently, implant associated infections.

Keywords:

Biofilm, biofilm embedded pathogens, copper, implants, antibiofilm, antimicrobial,

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