Novel “turn off-on” sensors for detection of DNA-acrylamide interaction using ZnS quantum dots as a phosphorescent probe

A novel “turn off-on” sensor for detection of interaction between DNA and acrylamide (ACR) was developed.In this method, L-cysteine capped Mn-doped ZnS quantum dots (QDs) were used as room temperature phosphorescentprobes. In the “turn-off” mode, ACR was absorbed onto the surface of QDs via electrostatic interaction, which caused aquenching effect of room-temperature phosphorescence signal by photoinduced electron-transfer mechanism. ACR wasremoved from the QDs’ surface with the addition of DNA. Thus, the phosphorescence emission of QDs was recoveredand the system was turned to the “turn-on” mode. The quenching mechanism of QDs by ACR was collisional (dynamic)and the quenching constant, binding constant, and binding site number were calculated as 3.2 × 10 4 M −1, 2.04 × 10 4M −1, and 1.2, respectively. An absorption spectrometric method was also used to evaluate ACR-DNA interaction andthe binding constant (K) was found as 2.4 × 10 5 M −1. The developed biosensor is simple, is free of interferences comingfrom autofluorescence and scattering light, and does not need any derivatization step or sample pretreatment.

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1. Mottram, D. S.; Wedzicha, B. L.; Dodson, A. T. Nature 2002, 419, 448-449.
2. Stadler, R. H.; Blank, I.; Varga, N.; Robert, F.; Hau, J.; Guy, P. A.; Robert, M. C.; Riediker, S. Nature 2002, 419, 449-450.
3. Keramat, J.; LeBail, A.; Prost, C.; Soltanizadeh, N. Food Bioprocess Technol. 2011, 4, 340-363.
4. Dybing, E.; Farmer, P. B.; Andersen, M.; Fennell, T. R.; Lalljle, S. P. D.; Muller, D. J. G.; Olin, S.; Peterson, B. J.; Schlatter, J.; Scholz, G. et al. Food Chem. Toxicol. 2005, 43, 365-410.
5. Alam, M. F.; Varshney, S.; Khan, M. A.; Laskar, A. A.; Younus, H. Int. J. Biol. Macromol. 2018, 113, 300-308.
6. Huang, J. H.; Wang, X. M.; Fei, D.; Ding, L. S. Appl. Spectrosc. 2010, 64, 1126-1130.
7. Ozluer, C.; Kara, H. E. S. J. Photoch. Photobio. B 2014, 138, 36-42.
8. Gao, X. L.; Patel, D. J. Q. Rev. Biophys. 1989, 22, 93-138.
9. Kara, H. E. S. Bioelectrochemistry 2014, 99, 17-23.
10. Yola, M. L.; Ozaltin, N. J. Electroanal. Chem. 2011, 653, 56-60.
11. Kuijt, J.; Ariese, F.; Brinkman, U. A. T.; Gooijer, C. Anal. Chim. Acta 2003, 488, 135-171.
12. Frasco, M. F.; Chaniotakis, N. Sensor 2009, 9, 7266-7286.
13. Resch-Genger, U.; Grabolle, M.; Cavaliere-Jaricot, S.; Nitschke, R.; Nann, T. Nat. Methods 2008, 5, 763-775.
14. Roya, Z.; Mansour, B.; Afshin, M.; Gamal, H. H. Clin. Biochem. 2011, 44, S223.
15. Gholami, S.; Kompany-Zareh, M. Anal. Bioanal. Chem. 2013, 405, 6271-6280.
16. Sinduja, B.; John, S. A. Spectrochim. Acta A 2018, 193, 486-491.
17. Muhammad, S.; Xu, G. H.; Wei, F. D.; Ma, Y. J.; Ma, Y. S.; Song, Y. Y.; Shi, M. L.; Xu, X. M.; Cen, Y.; Hu, Q. Nanomaterials 2017, 7, E358.
18. .Bajwa, N.; Mehra, N. K.; Jain, K.; Jain, N. K. Artif. Cell. Nanomed. B 2016, 44, 758-768.
19. Liu, X. T.; Na, W. D.; Qu, Z. Y.; Su, X. G. RSC Adv. 2016, 6, 85795-85801.
20. Meng, A.; Xu, Q. H.; Zhao, K.; Li, Z. J.; Liang, J.; Li, Q. D. Sensor. Actuat. B 2018, 255, 657-665.
21. Chen, L.; Zhang, X.; Zhang, C.; Zhou, G.; Zhang, W.; Xiang, D.; He, Z.; Wang, H. Anal. Chem. 2011, 83, 7316-7322.
22. Huang, S.; Lu, S.; Huang, C.; Sheng, J.; Zhang, L.; Su, W.; Xiao, Q. Sensor. Actuat. B 2016, 224, 22-30.
23. Li, D.; Xu, Y. M.; Zhang, L.; Tong, H. X. Int. J. Electrochem. Sc. 2014, 9, 7217-7227.
24. He, Y.; Wang, H. F.; Yan, X. P. Anal. Chem. 2008, 80, 3832-3837.
25. Şatana Kara, H. E.; Demirhan, B.; Er Demirhan, B. Turk. J. Chem. 2016, 40, 762-771.
26. Chung, J. H.; Ah, C. S.; Jang, D. J. J. Phys. Chem. B 2001, 105, 4128-4132.
27. Brouwer, A. M. Pure Appl. Chem. 2011, 83, 2213-2228.
28. Vesela, H.; Sucman, E. Czech J. Food Sci. 2013, 31, 401-406.
29. Zhao, T.; Hou, X. D.; Xie, Y. N.; Wu, L.; Wu, P. Analyst 2013, 138, 6589-6594.
30. Lakowicz, J. R. Principles of Fluorescence Spectroscopy; Plenum Press: New York, NY, USA, 1999.
31. Qiu, Y.; Qu, X.; Dong, J.; Ai, S.; Han, R. J. Hazard. Mater. 2011, 190, 480-485.
32. Kennard, O. Pure Appl. Chem. 1993, 65, 1213-1222.
33. Zhang, Y. L.; Zhang, X.; Fei, X. C.; Wang, S. L.; Gao, H. W. J. Hazard. Mater. 2010, 182, 877-885.
34. Zhang, G. W.; Wang, A. P.; Jiang, T.; Guo, J. B. J. Mol. Struct. 2008, 891, 93-97.
35. Ertas, N.; Kara, H. E. S. Biosens. Bioelectron. 2015, 70, 345-350.