In vitro effects of pesticide exposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes

The organophosphate target enzyme paraoxonase-1 (PON1) has been extensively studied in toxicology. Pesticides are organophosphate compounds that are commonly used in agriculture. They are also used as nerve gases, such as sarin and soman. Therefore, the effects of these compounds on enzymatic activity are well known. In the present study, we investigated the in vitro effects of fenoxaprop-p-ethyl, lambda cyhalothrin, imidacloprid, and dichlorvos pesticides on sheep liver microsomal PON1. The enzyme was purified approximately 141-fold with a specific activity of 1822.22 EU/mg proteins. The pesticides inhibited sheep liver PON1 in vitro, and the IC50 values for these compounds were 0.0103, 0.069, 0.157, and 0.2 m M, respectively.

In vitro effects of pesticide exposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes

The organophosphate target enzyme paraoxonase-1 (PON1) has been extensively studied in toxicology. Pesticides are organophosphate compounds that are commonly used in agriculture. They are also used as nerve gases, such as sarin and soman. Therefore, the effects of these compounds on enzymatic activity are well known. In the present study, we investigated the in vitro effects of fenoxaprop-p-ethyl, lambda cyhalothrin, imidacloprid, and dichlorvos pesticides on sheep liver microsomal PON1. The enzyme was purified approximately 141-fold with a specific activity of 1822.22 EU/mg proteins. The pesticides inhibited sheep liver PON1 in vitro, and the IC50 values for these compounds were 0.0103, 0.069, 0.157, and 0.2 m M, respectively.

___

  • Gauthier, E.; Fortier, I.; Courchesne, F.; Pepin, P.; Mortimer, J.; Gauvreau, D. Environmental Research Section A 2001, 86, 37–45.
  • Betarbet, R.; Sherer, T. B.; MacKenzie, G.; Garcia-Osuna, M.; Panov, A. V.; Greenamyre, J.T. Neuroscience 2000, 3, 1301–1306.
  • Maumbe, B. M.; Swinton, S. M. Social Science & Medicine 2003, 9, 1559–1571.
  • Mecdad, A. A.; Ahmed, M. H.; ElHalwagy, M. E. A.; Afify, M. M. M. Egyptian Journal of Forensic Sciences 2011, 1, 93–98.
  • Gauvreau, D. Interface 1987, 8, 16–21.
  • Durringhton, P. N.; Mackness, B.; Mackness, M. J. Arterioscler. Thromb. Vasc. Biol. 2001, 21, 473–80.
  • La Du, B. N.; Aviram, M.; Billecke, S. Chem.-Biol. Interact. 1999, 120, 379–88.
  • Gosta, L. G.; Cole, T. B.; Furlong, C. E. Acta Biomed. 2005, 12, 50–57.
  • Pornprom, T.; Mahatamnuchoke, P.; Usui, K. Pest Manag. Sci. 2006, 62, 1109–1115.
  • Henry, M. C.; Assi, S. B.; Rogier, C.; Dossou-Yovo, J.; Chandre, F.; Guillet, P.; Carnevale, P. Am. J. Trop. Med. Hyg. 2005, 73, 859–864.
  • Bai, D.; Lummis, S.C.R.; Leicht, W.; Breer, H.; Sattelle, D.B. Pestic. Sci. 1991, 33, 197–204.
  • Patel, S.; Bajpayee, M.; Pandey, A. K.; Parmar, D.; Dhawan, A. Toxicol. in vitro 2007, 21, 1409–1418.
  • Sinan, S.; Kockar, F.; Gencer, N.; Yıldırım, H.; Arslan, Biol. Pharm. Bull. 2006, 29, 1559–1563.
  • Ekinci, D.; Beydemir, S. Biol. Trace Elem. Res. 2010, 135, 112–120.
  • Arslan, M.; Gencer, N.; Arslan, O.; Guler, O. O. J. Enzym. Inhib. Med. Ch. 2012, 27, 722–729.
  • Mackness, B.; Davies, G. K.; Turkie, W.; Lee, E.; Roberts, D. H.; Hill, E.; Roberts, C.; Durrington, P. N.; Mackness, M. I. Arterioscler. Thromb. Vasc. Biol. 2001, 21, 1451–1457.
  • Mackness, B.; Durrington, P.; McElduff, P.; Yarnell, J.; Azam, N.; Watt, M.; Mackness, M. Circulation 2003, 107, 2775–2779.
  • Aviram, M.; Rosenblat, M.; Bisgaier, C. L.; Newton, R. S.; Primo-Parmo, S. L.; La Du, B. N. J. Clin. Invest. 1998, 101, 1581–1590.
  • Deakin, S.; James, R. Clin. Sci. 2004, 107, 435–447.
  • Chait, A.; Han, C. Y.; Oram, J. F.; Heinecke, J. W. J. Lipid Res. 2005, 46, 389–403.
  • Kasprzak, M.; Iskra, M.; Majewski, W.; Wielkoszynski, T. Clin. Biochem. 2009, 42, 50–56.
  • Marsillach, J.; Camps, J.; Ferr´ e, N.; Beltran, R.; Rull, A.; Mackness, B.; Mackness, M.; Joven, J. BMC Gastroenterology 2009, 9, 3.
  • Ekinci, D.; Beydemir, S.; Alım, Z. Pharmacol. Rep. 2007, 59, 580–587.
  • Akkemik, E.; Budak, H.; Ciftci, M. J. Enzym. Inhib. Med. Ch. 2010, 25, 871–875.
  • Alıcı, H. A.; Ekinci, D.; Beydemir, S. Clin. Biochem. 2008, 41, 1384–1390.
  • Ekinci, D.; Beydemir, S. Eur. J. Pharmacol. 2009, 617, 84–89.
  • Gonzalvo, M. C.; Gil, F.; Hernhdez, A. F.; Villanueva, E.; Pla, A. Chem.-Biol. Interact. 1997, 105, 169–179.
  • Singh, S.; Kumar, V.; Thakur, S.; Banerjee, B. D.; Rautela, R. S.; Grover, S. S.; Rawat, D. S.; Pasha, S. T.; Jain, S. K.; Ichhpujani, R. L.; et al. Toxicol. Appl. Pharm. 2011, 252, 130–137.
  • Gauthier, E.; Fortier, I.; Courchesne, F.; Pepin, P.; Mortimer, J.; Gauvreau, D. Environ. Res. 2001, 86, 37-45.
  • Betarbet, R.; Sherer T. B.; MacKenzie, G.; Garcia-Osuna, M.; Panov, A. V.; Greenamyre, J. T. Nat. Neurosci. 2000, 3, 1301–1306.
  • S ¸ent¨ urk, M.; G¨ ul¸ cin, I.; Beydemir, S.; K¨ ufrevio˘ glu, O. I.; Supuran, C. T. Chem. Biol. Drug Des. 2001, 77, 494–499. Yılmaz, H.; Ciftci, M.; Beydemir, S.; Bakan, E. Prep. Biochem. Biotech. 2002, 32, 287–301.
  • G¨ ul¸ cin, I.; Beydemir, S.; C ¸ oban, T. A.; Ekinci, D. Fresen. Environ. Bull. 2008, 17, 1283–1287.
  • Alım, Z.; Beydemir, S. Arch. Physiol. Biochem. 2012, 118, 244–252.
  • Rodrigo, L.; Gil, F.; Hernandez, A. F.; Maria, A.; Vazquez, J.; Pla, A. Biochem. J. 1997, 321, 595–601.
  • Ekinci, D.; Sent¨ urk, M.; Beydemir, S.; K¨ ufrevioglu, O. I.; Supuran, C. T. Chem. Biol. Drug Des. 2010, 76, 552–558. Erol, K.; Gencer, N.; Arslan, M.; Arslan, O. Nanomedicine and Biotechnology 2013, 41, 125–130.
  • Renault, F.; Chabri` ere, E.; Andrieu, J. P.; Dublet, B.; Masson, P.; Rochu, D. J Chromatogr. B 2006, 836, 15–21. Bradford, M. M. Anal. Biochem. 1976, 72, 248–51.
  • Laemmli, D. K. Nature 1970, 227, 680–3.
  • Hisar, O.; Beydemir, S.; Gulcin, I.; Kufrevioglu, O. I.; Supuran, C. T. J. Enzyme Inhib. Med. Ch. 2005, 20, 35–39.
Turkish Journal of Chemistry-Cover
  • ISSN: 1300-0527
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK