Immobilized metal ion affinity nanospheres for α-amylase immobilization
Immobilized metal ion affinity nanospheres for α-amylase immobilization
Immobilized metal chelate affinity chromatography (IMAC) support was practiced for α -amylase immo- 2+ 2+bilization. Poly(hydroxyethylmethacrylate-methacryloylamidotryptophan)-Ni [p(HEMA-MAT)-Ni ] nanospheres, average diameter 100 nm, were produced by surfactant free emulsion polymerization. Characterizations of p(HEMA- MAT)-Ni 2+ nanospheres were carried out by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). In addition, average particle size, size distribution, and surface charge were specified. The amount of N-methacryloylamidotryptophan (MAT) incorporated to polymer was determined as 1.95 mmol/g polymers by using nitrogen stoichiometry. The specific surface areas of poly(hydroxyethylmethacrylate) [p(HEMA)] and p(HEMA-MAT) 2 2nanospheres were calculated as 1856 m /g and 1914 m /g, respectively. Protein adsorption increased with increasing initial protein concentration and maximum α -amylase adsorption on p(HEMA-MAT)-Ni 2+ nanospheres was observed at pH 4.0. Both free and immobilized α -amylase showed pH optimum at pH 7.0. It was determined that the immobilized α -amylase had better thermostability than the free one. Immobilization of the enzyme did not significantly change the kinetic parameters. The storage stability of α -amylase increased upon immobilization. It was also observed that 2+p(HEMA-MAT)-Ni nanospheres can be repeatedly used for α -amylase immobilization.
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- Schenck, F. W.; Hebeda, R. E. Starch Hydrolysis Products: Worldwide Technology, Production and Applications, VCH, New York, 1992.
- Windish, W. W.; Mhatre, N. S. Advances in Applied Microbiology, In: Wayne, W. U. Ed., 1965.
- Fogarty, W. M.; Kelly, C. T. In Topics in Enzyme and Fermentation Biotechnology; Wiseman, A., Ed., 1979.
- Vihinen, M.; Mantsala, P. Crit Rev. Biochem. Mol. Biol. 1989, 24, 329418.
- Lonsane, B. K.; Ramesh, M. V. Adv. Appl. Microbiol. 1990, 35, 156.
- Nikolov, Z. L.; Reily, P. J. In Biocatalysts for Industry; Dordick, J. S., Ed. Plenum Press, New York, 1991.
- Liu, Y.; Jia, S.; Ran, J.; Wu, S. Catal. Commun. 2010, 11, 364367.
- Yamamoto, T. In Handbook of Amylases and Related Enzymes: Their Source, Isolation Methods, Properties and Applications; Amylase Research Society of Japan, Ed., Pergamon Press, Oxford, 1988.
- Bahar, T.; Celebi, S. S. Enzyme Microb. Technol. 2000, 26, 2833.
- Roig, M. G.; Kennedy, J. F.; Garaita, M. G. J. Biomater. Sci. Polym. Ed. 1994, 6, 661673.
- Ida, J.; Matsuyama, T.; Yamamoto, H. Biochem. Eng. J. 2000, 5, 179184.
- Yang, Y.; Chase, H. A. Biotechnol. Appl. Biochem. 1998, 28, 145154.
- Chen, J. P.; Sun, Y. M.; Chu, D. H. Biotechnol. Prog. 1998, 14, 473478.
- Bryjak, J. Bioprocess Eng. 1995, 13, 177181.
- Mateo, C.; Palomo, J. M.; Fernandez-Lorente, G.; Guisan, J. M.; Fernandez-Lafuente, R. Enzyme Microb. Technol. 2007, 40, 14511463.
- Akgol, S.; Ozturk, N.; Denizli, A. J. Appl. Polym. Sci. 2010, 115, 16081615.
- Cong, L.; Kaul, R.; Dissing, U.; Mattiasson, B. J. Biotechnol. 1995, 42, 7584.
- Tischer, W.; Kashe, V. Biotechnology 1999, 17, 326335.
- Ye, P.; Xu, Z. K.; Che, A. F.; Wu, J.; Seta, F. Biomaterials 2005, 26, 63946403.
- Rebros, V.; Rosenberg, M.; Mlichova, Z.; Kristofıkova, L. Food Chem. 2007, 102, 784787.
- Sankalia, V.; Mashru, V.; Sankalia, J. M.; Sutariya, V. B. Eur. J. Pharm. Biopharm. 2007, 65, 215232.
- Peng, K.; Hidajat, K.; Udin, M. S. J. Colloid Interface Sci. 2004, 271, 277283.
- Choi, S. W.; Kwon, H. Y.; Kim, W. S.; Kim, J. H. Colloids Surf. A 2002, 201, 283289.
- Ozturk, N.; Akgol, S.; Arisoy, M.; Denizli, A. Sep. Purif. Technol. 2007, 58, 8390.
- Kim, J.; Grate, J. W.; Wang, P. Chem. Eng. Sci. 2006, 61, 10171026.
- Tuzmen, N.; Kalburcu T.; Denizli A. Process Biochem. 2012, 47, 2633.
- Porath, J.; Carlson, J.; Olsson, I.; Belfrage, G. Nature 1975, 258, 598599.
- Gaberc-Porekar, V.; Menart, V. J. Biochem. Biophys. Methods 2001, 49, 335360.
- Arnold, F. H. Bio. Technology 1991, 9, 150155.
- Gutierrez, R. E.; Martin del Vale, M.; Galan, M. A. Sep. Purif. Rev. 2007, 36, 71111.
- Kubota, N.; Nakagawa, Y.; Eguchi, Y. J. Appl. Polym. Sci. 1996, 62, 11531160.
- Lawal, O. S. Food Chem. 2006, 95, 101107.
- Ueda, E. K. M.; Gout, P. W.; Morganti, L. J. Chromatogr. A 2003, 988, 123. Mosbach, K. Sci. Am. 1971, 224, 2633.
- He, D.; Cai, Y.; Wei, W.; Nie, L.; Yao, S. Biochem. Engineer. J. 2000, 6, 711.
- Tanyolac, D.; Yuruksoy, B. I.; Ozdural, A. R. Biochem. Eng. J. 1998, 2, 179186.
- ONeill, S. P.; Dunnill, P.; Lilly, M. D. Biotechnol. Bioeng. 1971, 13, 337352.
- Lopez, G. P.; Ratner, B. D.; Rapoza, R. J.; Horbett, T. A. Macromol. Symp. 1993, 26, 32473253.
- Arica, M. Y.; Senel, S.; Alaeddinoglu, N. G.; Patir, S.; Denizli, A. J. Appl. Polym. Sci. 2000, 75, 16851692.
- Aksoy, S.; Tumturk, H.; Hasirci, N. J. Biotechnol. 1998, 60, 3746.
- Turunc, O.; Kahraman, M. V.; Akdemir, Z. S.; Kayaman-Apohan, N.; Gungor, A. Food Chem. 2009, 112, 992997.
- Kara, A.; Osman, B.; Yavuz, H.; Be ̧sirli N., Denizli A. React. Funct. Polym. 2005, 62, 6168.
- Chang, M. Y.; Juang, R. S. Process Biochem. 2004, 39, 10871090.
- Reddy, K. R. C.; Kayastha, A. M. J. Mol. Catal. B: Enzym. 1994, 38, 104112.