Development of a biosensor platform based on ITO sheets modified with 3-glycidoxypropyltrimethoxysilane for early detection of TRAP1
Development of a biosensor platform based on ITO sheets modified with 3-glycidoxypropyltrimethoxysilane for early detection of TRAP1
The aim of this research was to design an electrochemical immunosensor for determination of tumournecrosis factor receptor-associated protein-1(TRAP1) antigen, a heat shock protein linked to tumour necrosis factor.The indium-tin oxide covered polyethylene terephthalate (ITO-PET) electrode surface was cleaned and was prepared forthe introduction of hydroxyl groups on its surface by using NH4 OH/H2 O2 /H2 O. As a silanization agent for covalentattachment of anti-TRAP1 on the surface of the ITO working electrode, 3-glycidoxypropyltrimethoxysilane (3-GOPS)was used. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterizethe immobilization steps. A variety of parameters, 3-GOPS and anti-TRAP1 concentrations, and anti-TRAP1 andTRAP1 incubation durations were optimized. After determining the optimum conditions, characterization studies suchas repeatability, reproducibility, regeneration, square wave voltammetry, and single frequency impedance were performed.The electrochemical immunosensor has presented an extremely wide determination range for TRAP1 from 0.1 pg/mL to 100 pg/mL.
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- 1. Lin MH, Shen KY, Liu BS, Chen IH, Sher YP. Immunological evaluation of a novel HLA-A2 restricted phosphopeptide of tumor associated Antigen, TRAP1, on cancer therapy. Vaccine 2019; X (1): 100017.
doi: 10.1016/j.jvacx.2019.100017
- 2. Li S, Sun X, Xu J, Gu Y, Liu S. Association study of TRAP1 gene polymorphisms with susceptibility and
glucocorticoid efficacy of systemic lupus erythematosus. Gene 2018; 671: 117-126. doi: 10.1016/j.gene.2018.05.109
- 3. Lin MH, Shen KY, Liu BS, Chen IH, Sher YP. Immunological evaluation of a novel HLA-A2 restricted phosphopeptide of tumor associated Antigen, TRAP1, on cancer therapy. Vaccine 2019; X (1): 100017.
doi: 10.1016/j.jvacx.2019.100017
- 4. Rondanin R, Lettini G, Oliva P, Baruchello R, Costantini C et al. New TRAP1 and Hsp90 chaperone inhibitors
with cationic components: preliminary studies on mitochondrial targeting. Bioorganic & Medicinal Chemistry
Letters 2018; 28 (13): 2289-2293. doi: 10.1016/j.bmcl.2018.05.031
- 5. Wu H, Liao J, Li Q, Yang M, Zhao M. Epigenetics as biomarkers in autoimmune diseases. Clinical Immunology
2018; 196: 34-39. doi: 10.1016/j.clim.2018.03.011
- 6. Aydın EB, Sezgintürk MK. Indium tin oxide (ITO): a promising material in biosensing technology. TrAC Trends
in Analytical Chemistry 2017; 97: 309-315. doi: 10.1016/j.trac.2017.09.021
- 7. Aydın EB, Sezgintürk MK. A comparison between LP (GMA) and CLP (GMA) polymer composites as an
immobilization matrix for biosensing applications: A model immunosensor for IL 1α. Analytica Chimica Acta
2019. doi: 10.1016/j.aca.2019.05.049
- 8. Aydın M, Aydın EB, Sezgintürk MK. Electrochemical immunosensor for CDH22 biomarker based on benzaldehyde substituted poly (phosphazene) modified disposable ITO electrode: a new fabrication strategy for biosensors. Biosensors and Bioelectronics 2019; 126: 230-239. doi:
10.1016/j.bios.2018.10.051
- 9. Karaboğa MNS, Sezgintürk MK. Cerebrospinal fluid levels of alpha-synuclein measured using a poly-glutamic acidmodified gold nanoparticle-doped disposable neuro-biosensor system. Analyst 2019; 144 (2): 611-621. doi: 10.1039
/ C8AN01279B
- 10. Lee JH, Nguyen H B-C, Ko E, Kim JH, Seong GH. Fabrication of flexible, transparent silver nanowire electrodes for amperometric detection of hydrogen peroxide. Sensors Actuators B: Chemical 2016; 224: 789-797. doi:
10.1016/j.snb.2015.11.006
- 11. Li HH, Wang HH, Li WT, Fang XX, Guo XC et al. A novel electrochemical sensor for epinephrine based on
three dimensional molecularly imprinted polymer arrays. Sensors Actuators B Chem 2016; 222: 1127-1133. doi:
10.1016/j.snb.2015.08.018
- 12. Zhou N, Chen H, Li J, Chen L. Highly sensitive and selective voltammetric detection of mercury (II) using an
ITO electrode modified with 5-methyl-2-thiouracil, graphene oxide and gold nanoparticles. Microchimica Acta
2013; 180: 493-499. doi: 10.1007/s00604-013-0956-0
- 13. dos Santos MB, Azevedo S, Agusil J, Prieto-Simon B, Sporer C et al. Label-free ITO-based immunosensor for
the detection of very low concentrations of pathogenic bacteria. Bioelectrochemistry 2015; 101: 146-152. doi:
10.1016/j.bioelechem.2014.09.002
- 14. Bagbi Y, Sharma A, Bohidar H, Solanki PR. Immunosensor based on nanocomposite of nanostructured zirconium
oxide and gelatin-A. International Journal of Biological Macromolecules 2016; 82: 480-487.
doi: 10.1016/j.ijbiomac.2015.10.074
- 15. Singh A, Choudhary M, Singh M, Verma H, Singh SP et al. DNA Functionalized direct electro-deposited gold
nanoaggregates for efficient detection of Salmonella typhi. Bioelectrochemistry 2015; 105: 7-15.
doi: 10.1016/j.bioelechem.2015.03.005
- 16. Yagati AK, Pyun JC, Min J, Cho S. Label-free and direct detection of C-reactive protein using reduced graphene
oxide-nanoparticle hybrid impedimetric sensor. Bioelectrochemistry 2016; 107: 37-44.
doi: 10.1016/j.bioelechem.2015.10.002
- 17. Özcan B, Sezgintürk MK. A novel label free immunosensor based on single-use ITO-PET electrodes for detection
MAGE1 protein. Journal of Electroanalytical Chemistry 2017; 792: 31-38. doi: 10.1016/j.jelechem.2017.03.036
- 18. Demirbakan B, Sezgintürk MK. A sensitive and disposable indium tin oxide based electrochemical immunosensor
for label-free detection of MAGE-1. Talanta 2017; 169: 163-169.doi: 10.1016/j.talanta.2017.03.076